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Biomedical subjects

I O Olubuyide

Publications and source records attributed to I O Olubuyide.

At least 19 recordsLinked to original sources

The natural history of primary liver cell carcinoma: a study of 89 untreated adult Nigerians.

A Nigerian series of 890 patients with primary liver cell carcinoma, seen during the recent three years, has been examined with a view to establishing the natural history of the tumour in untreated cases. There were 60 males and the mean age of all the patients was 50 years. Hepatitis B surface antigen was positive in 70 pc of tested patients and there were higher pathologic levels of aflatoxins in these patients when compared to normal controls. Liver cirrhosis was associated with 81 pc of patients. Alcohol and smoking were unlikely to be aetiologically important in these patients. The macroscopic type of tumour was mainly diffusely nodular and the commonest microscopic pattern was the characteristic trabecular pattern. Metastases were present in 52 pc of the patients and were mainly to the lungs. Due to late presentation and underlying cirrhosis, most patients were critically ill with high incidence of ascites, jaundice and hepatic precoma. The mean survival time of all patients was six months after onset of the initial symptoms to death and only three weeks after admission to death. The major causes of death were advanced cancer in 78 pc, hepatic failure in 48 pc and rupture of tumour, 39 pc. These observations clearly show that the prognosis of liver cancer is dismal in this environment, as elsewhere. Medical education on earlier presentation in hospital and early operative removal of the tumour should be emphasised. It is suggested that an attempt through immunisation should be employed to reduce the incidence of liver cancer in the population.

Adolescent

Abnormal lipid and lipoprotein patterns in liver cirrhosis with and without hepatocellular carcinoma.

Plasma lipid and lipoprotein profile was determined in 12 cirrhotics, 15 patients with cirrhosis and hepatocellular carcinoma (HCC) and 20 healthy volunteers. When compared with controls plasma total cholesterol (TC), high density lipoprotein cholesterol (HDLC), high density lipoprotein phospholipids (HDLPL), HDLPL/PL levels were low, phospholipid (PL) was normal and HDLC/TC and PL/TC were high in cirrhosis. In cirrhotics with HCC, TC, PL, PL/TC levels were elevated while HDLC, HDPL, HDLC/TC and HDLPL/PL were normal. A comparison within the patient groups showed that in cirrhosis alone, the levels of TC, HDLC, PL, HDLPL and HDLPL/PL were lower and PL/TC level was higher than in cirrhotics with HCC. Plasma albumin levels showed a negative correlation with PL/TC and a positive correlation with HDL-cholesterol in cirrhosis. In cirrhosis with HCC plasma, phospholipid levels showed a significant negative correlation with total bilirubin and alanine aminotransferase. The variations in the level of plasma lipids and lipoproteins may assist in describing the nature of these two forms of liver disease.

Adult

Investigation of the assay of AFB1-albumin adducts using proteolysis products in ELISA.

Epidemiological evidence of the involvement of aflatoxins in the aetiology of human liver cancer has led to an increasing interest in the development of appropriate techniques for monitoring human exposure. The assay for aflatoxin adducts in albumin has a better potential for assessing long-term exposure than analyses of urine samples, and several protocols for ELISA of these adducts, following proteolysis of albumin, have been examined. However, there is usually an incomplete release of a major adduct, aflatoxin-lysine, even after prolonged hydrolysis, and the adduct is very unstable under some conditions of proteolysis for unknown reasons. Therefore, before such techniques can be recommended for general application, the significance of such factors in the quantitive estimation of aflatoxin adducts needs to be evaluated. This study has detected the presence of a considerable fraction of aflatoxin-modified material, produced by proteolysis of in vivo aflatoxin-modified rat albumin or in vitro modified bovine albumin, and which is not recognized in ELISA by an anti-aflatoxin polyclonal antibody having a wide spectrum of aflatoxin metabolite detection. This fraction increases in parallel with the proteolysis protocols.

Aflatoxin B1

The isolation and culture of DHBV-infected embryo and duckling hepatocytes and the effect of aflatoxin B1 or irradiation on these cells.

The preparation of primary cultures of control and DHBV-infected duck hepatocytes from embryos and young ducklings is described. Cultures of both embryo and duckling hepatocytes secreted duck serum proteins. Cultures of hepatocytes established from ducklings maintained initial morphology for up to 3 weeks in culture and also exhibited high levels of metabolism of aflatoxin B1. Embryonic cell cultures rapidly lost ability to metabolise AFB1 and became overgrown by spindle-shaped cells. Both embryo and duckling cell cultures secreted infective DHBV, and had intracellular replicative forms of the virus. No integration of the virus into the duck genome was observed, and attempts to induce viral integration in the duckling hepatocytes using irradiation and aflatoxin B1 toxicity were unsuccessful. The results of the study lend further support to the suggestion that the rarity of liver cancer in DHBV-infected experimental ducks is related to an innate resistance of the hepatocytes to develop DHBV-DNA integration. Another possibility may be related to the lower oncogenic potential of the DHBV strain used for the study. However DHBV infected duckling hepatocytes would appear to offer a suitable material for studying viral replication and mechanisms of aflatoxin B1 toxicity during prolonged cell culture.

Aflatoxin B1

Changes in plasma high density lipoprotein cholesterol and phospholipid in acute viral hepatitis and cholestatic jaundice.

Forty-four male and female subjects aged 22-57 years were studied. Thirteen patients had acute viral hepatitis, and eleven patients had cholestatic jaundice due to carcinoma of the head of the pancreas. Twenty healthy volunteers who served as controls were also included. In hepatitis patients, the mean plasma levels of total cholesterol (TC) and the high density lipoprotein (HDL)-phospholipid/phospholipid (HDLPL/PL) ratio were reduced, and HDL-cholesterol (HDLC), HDL-phospholipid (HDLPL) and the phospholipid/total cholesterol (PL/TC) ratio were normal, while total phospholipid (PL) levels and the HDLC/TC ratio were significantly increased compared to the control values. In patients with cholestatic jaundice the mean plasma total cholesterol, phospholipid and HDLC levels were elevated, and HDLPL/PL, HDLPL, HDLC/TC and PL/TC remained normal compared to the control values. A comparison within the patient groups showed that plasma TC, PL and HDLC levels were significantly increased in cholestatic jaundice when compared with the corresponding levels in hepatitis patients. The mean plasma levels of HDLPL, HDLC/TC and PL/TC did not show any significant variation within the patient groups. Alkaline phosphatase (ALP) correlated positively with TC, and total protein correlated negatively with TC and HDLPL, while albumin correlated negatively with TC, HDLC and HDLPL in cholestatic jaundice. Alanine amino-transferase (ALAT) also correlated positively with PL in cholestatic jaundice, while albumin correlated positively with TC in hepatitis. The results suggest that lipoproteins might be metabolized differently in these two forms of cholestasis.

Acute Disease

Disease pattern in the elderly.

Analysis of the disease pattern in elderly patients was carried out over a seven-year period (1980-1986) on the medical wards of the University College Hospital, Ibadan, Nigeria. A total of 2,676 patients were reviewed. Cardiac, neoplastic and infective diseases accounted for the most common diseases. Early diagnosis and treatment as well as improvement in preventive medicine are suggested ways to a vigorous healthy old age with concomitant reduction of morbidity and mortality in this age group.

Aged

Diseases of the middle-aged population: a study of 4,184 patients.

Within a seven-year period (1980-1986), 4,184 Nigerians aged 31-60 years were admitted into the medical wards of the University College Hospital, Ibadan, Nigeria. The clinical notes of these patients were analysed and the principal diagnoses for each case was coded in accordance with the International Classification of Diseases. Cardiovascular, infective, neoplastic and neuro-muscular diseases constituted the commonest disorders being 70pc of all diseases at admission in this age group. The relevance of these findings in the light of the pattern of diseases elsewhere is discussed.

Age Factors

The chest radiograph in primary liver cell carcinoma.

In a prospective study, 38 consecutive cases of primary liver cell carcinoma were seen over one year (1988) at the liver unit of the University College Hospital, Nigeria. An analysis of the chest X-ray findings was made. Normal chest X-rays were found in 23.7% of cases. Abnormal findings included elevated diaphragm (63.2%), pulmonary metastases (18.4%), and pleural effusion (18.4%), perhaps the highest ever so reported. It may be concluded that, in a middle-aged man with a hard irregular hepatomegaly, the findings of the triad of elevated diaphragm, pulmonary metastasis and pleural effusion on chest X-ray should make one very suspicious of liver cancer. The differential diagnoses of these radiological findings are briefly discussed.

Adolescent

A case-controlled study of the current role of cigarette smoking and alcohol consumption in primary liver cell carcinoma in Nigerians.

This paper describes the findings of a case-controlled study of 100 patients with primary liver cell carcinoma, sex- and age-matched with another 100 patients seen at the orthopaedic clinic of the University College Hospital, Ibadan. Contrary to widespread belief, the relative risks of cigarette smoking and alcohol consumption were not found to be statistically significant. The implication of this on the possible aetiology of primary liver cancer is discussed.

Adult

Amoebic cases at autopsy and factors that might have contributed to death at Ibadan.

Twenty-one cases of amoebiasis seen at autopsy at the University College Hospital, Ibadan, over a 10-year period are reviewed. They constituted 0.59 percent of 3,556 autopsies performed during this period. Delay in seeking medical advice and in diagnosis and commencement of appropriate therapy were some of the factors contributing to the mortality in 16 (76pc) cases. Furthermore, Chronic renal failure, Cirrhosis, Lymphoma, Pregnancy and Sickle cell disease were also contributory factors to mortality in 7 (33pc) cases. With the progressive deterioration of the economics of Nigeria, urban migration and the increasing size of urban slums with crowded unhygienic conditions, the spread of this infection may accelerate and so result in greater mortality in the future.

Adolescent

The causes of death in an elderly African population.

Information on the causes of death in elderly populations is sparse or not available for most tropical African countries. Analysis of the causes of death of patients aged 65 years and above on the medical and surgical wards of the University College Hospital, Ibadan, Nigeria was therefore carried out over a 10-year period (1977-1986). A total of 223 cases were reviewed. Neoplastic, cardiac and infective diseases accounted for the majority of deaths. Improvement in health education, early diagnosis and treatment as well as preservation of cultural dietary habits are suggested ways of reducing a rise in degenerative diseases and the number of deaths.

Aged

The value of C-reactive protein in the diagnosis of intestinal perforation in typhoid fever.

Serum C-reactive protein concentrations were measured in 28 Nigerian patients with typhoid fever on presentation at hospital and at intervals thereafter. Five of the 28 (18%) were subsequently diagnosed as suffering from ileal perforation. These patients had concentrations of C-reactive protein during the first 24 h of admission which were significantly higher (mean = 229 mg/L) than those without perforation (mean = 91 mg/L). C-reactive protein may have a role in the prediction of early diagnosis of perforation in patients with typhoid fever.

C-Reactive Protein

Gastrointestinal malignancies in a young tropical African population.

The records of 695 patients with confirmed gastroenterological malignancies over a 10 year period (January 1976-December 1985) were analysed. Of these, 264 patients (37.98%) were below the age of 40 years. Primary liver cell carcinoma was the commonest malignancy while gallbladder and oropharyngeal cancers were the least common. Most of the patients (66.3%) were in their fourth decade. The prognosis was poor as most of the patients presented late.

Adolescent

Haemoglobin genotypes and primary liver cell carcinoma in Africans.

The distribution of haemoglobin genotype in 200 patients with primary liver cell carcinoma (PLCC) did not show any statistical difference from those of 200 age and sex-matched controls. It is therefore concluded that haemoglobin genotype is unlikely to play a significant role in the development of PLCC in Africans.

Africa

Malignant giant gastric ulcer in a Nigerian.

A case of malignant giant gastric ulcer in a 48-year-old Nigerian is reported. This is a rare lesion in the African. The clinico-radiological features that would heighten the clinical suspicion of this condition are highlighted.

Adenocarcinoma, Mucinous

Measurement of sialyl-transferase activity in isolated colonic mucosal cells of the rat.

An assay for sialyl-transferase activity in isolated rat colonic mucosal cells has been developed. Total and specific activity with asialobovine sub-mandibular gland glycoprotein and endogenous substrates was approximately two-fold higher in the proximal, relative to the distal, colon. These activities were similar when asialo-alpha-acid glycoprotein was used as substrate. Endogenous activity was approximately 10-fold lower than with exogenous substrates in both proximal and distal colonic segments. Analysis of total and specific sialyl-transferase activity up to 7 weeks after jejunoileal bypass (JIB) and sham operation showed a marked increase at the first week, decreasing toward normal by week 7. Similar differences between proximal and distal colon, and with the type of substrate, were found with both groups of operated animals. Histochemical analysis showed small elevation after 1 week for sialo and sulphomucins after the JIB operation only.

Animals

Effect of non-steroidal anti-inflammatory agents on the upper gastro-intestinal tract: gastroscopic findings in Nigerians.

The effect of 4 non-steroidal anti-inflammatory agents, viz Aspirin, Piroxicam, Ibuprofen and Indomethacin on the gastric mucosa of the African was studied on 34 volunteers who had no previous symptoms of acid peptic diseases. Aspirin caused the worst lesions while piroxicam caused the least lesions. The indiscriminate prescription of these drugs, particularly drugs containing acetosalicylic acid should be avoided.

Adult

An autopsy survey of peptic ulcer disease at Ibadan.

The frequency of peptic ulcer disease remains highly controversial worldwide and from this environment there is little information either on the mortality and the factors contributory to death from the disease or on the associated conditions in patients with asymptomatic ulcers. The purpose of this study was to focus on these issues in an autopsy population at the University College Hospital, Ibadan, Nigeria. The overall incidence of peptic ulcer disease was 5 percent and the disease was a direct cause of death in 1.5 percent of the autopsy population. Bleeding was the most frequent cause of death from peptic ulcer disease and was responsible for 72.2 percent of the deaths due directly to ulcer disease. This was followed by ulcer perforation found in 25.9 percent of the deaths. The factors contributing to mortality include non-compliance with treatment and delay in presentation at the hospital. Correlations have been demonstrated in all age groups between asymptomatic peptic lesions and severe pathological conditions such as typhoid septicaemia. The relevance of this later finding is briefly discussed.

Adolescent