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Biomedical subjects

I Ohmori

Publications and source records attributed to I Ohmori.

At least 19 recordsLinked to original sources
PubMed1989

[A histological observation of the primary teeth with enamel hypoplasia resulting from perinatal disturbance].

Two primary teeth with enamel hypoplasia from two cerebralpalsied children were subjected to histological examination. One primary tooth was B and the other was C. The location of enamel hypoplasia in these teeth was considered to coincide with the neonatal line reaching the enamel surface. The purpose of this study was to examine the location of the enamel hypoplasia in these teeth in relation to the neonatal line, and to detail the histological structure of the teeth. They were examined morphologically as well as histologically, by using a stereoscopic microscope, SEM, a light microscope and a microradiogram. The results obtained were as follows: 1. The size of the tooth crown of these teeth was smaller than the normal standard, but the form of tooth crown was normal. 2. Based on streomicroscopic observation, enamel hypoplasia was found on the labial and lingual surfaces in B, and on labial surface only in C. In B, the enamel hypoplasia on labial surface was extended widely to incisal 2/3 of the crown, while that on the lingual surface was minimum. In C, there were two spots of enamel hypoplasia located on the mesial and distal incisal edges respectively. 3. Based on SEM observation, the hypoplastic enamel was formed right after their birth. 5. Based on microradiographic observation, the neonatal lines were radiolucent, and the adjacent areas of the neonatal line did not show any poor calcification. 6. From these histological observations, it was found that the perinatal disturbance directly affected the tooth enamel formation and these enamel hypoplasias were positively caused by the cerebral palsy of these children.

Cerebral Palsy↗
PubMed1986

Purification and immunofluorescence localization of the mutant gene product of a Tetrahymena cdaA1 mutant affecting cell division.

A division arrest mutant, cdaA, of Tetrahymena thermophila is known to have a ts-defect in the formation of the fission zone which determines the position of the fission plane. A protein (Mr = 85,000; pI = 4.7, designated as p85) has recently been identified in our laboratory as a possible gene product of the cdaA locus by two-dimensional gel electrophoresis and genetic experiments (Ohba et al., submitted). In the present research, we have isolated p85, prepared its antibody, and demonstrated that in wild-type cells or in cdaA cells at permissive temperature, immunofluorescence for p85 appears on the equatorial basal bodies at the predicted fission zone just before formation of the zone. In such a case, the fission zone appears to be formed just anterior to the fluorescence-associated basal bodies, and then constriction of the division furrow occurs at the zone. However, in cdaA cells at the restrictive temperature, the equatorial p85 deposit and subsequent fission zone formation and furrowing do not occur at all. Thus, we conclude that p85 plays a key role in the formation of the fission zone and in the positioning of the equatorial fission line.

Animals↗
PubMed1976

Effect of the methyl methacrylate-tributylborane sealant in preventing occlusal caries.

A newly developed pit and fissure sealant, named Enamite, was studied technologically and clinically. Major ingredients of the sealant are a solution of 3% 2-hydroxy-3-beta-naphthoxypropyl methacrylate in methyl methacrylate and poly (methyl methacrylate) powder, using tributylborane as an initiator. In comparison with other commercially available sealants, Enamite showed much greater adhesiveness to enamel, which was confirmed by scanning electron micrographic observations. The sealant was applied to 161 first molars in 99 children, aged 6-8 years, to determine the caries preventive as well as the caries retardation effect. Among the treated teeth, 98 were intact and the remaining had incipient caries. The right side teeth of each subject was assigned to the test side and the other side served as a control. Each child was evaluated every 6 months by clinical exploration and bite-wing X-ray survey. At the final examination, the electric conductivity test was also used for the caries detection. After 2 years the sealant was effective in preventing 57.1% of the occlusal caries on treated compared with control teeth. When evaluation was made by including the caries retardation effect--teeth initially carious but remaining sealed after 2 years were counted as effective--, the sealant was effective in preventing or retarding 96.3% of the occlusal caries.

Adhesiveness↗