Biomedical subjects

I P Clark

Publications and source records attributed to I P Clark.

6 recordsLinked to original sources

PubMed2005

Identification and reactivity of the triplet excited state of 5-hydroxytryptophan.

Both the neurotransmitter serotonin and the unnatural amino acid 5-hydroxytryptophan (5HT), contain the 5-hydroxyindole chromophore. The photochemistry of 5HT is being investigated in relation to the multiphoton excitation of this chromophore to produce a characteristic photoproduct with green fluorescence ('hyperluminescence'). Laser flash photolysis (308 nm) of 5HT in aqueous solution at neutral pH produces both the neutral 5-indoloxyl radical (lambda(max) 400-420 nm) and another transient absorption with lambda(max) 480 nm and lifetime of 2 micros in deaerated solutions. Based on quenching by oxygen and beta-carotene, the species at 480 nm is identified as the triplet excited state of 5HT. In acidic solution a new oxygen-insensitive intermediate with lambda(max) 460 is assigned to the radical cation of 5HT. Time-resolved measurements of luminescence at 1270 nm have shown that the triplet state of 5HT is able to react with oxygen to form singlet excited oxygen (1O2*) with a quantum yield of approximately 0.1. However, 5HT has also been found to be an effective quencher of singlet oxygen with a second order rate constant of 1.3 x 10(8) dm3 mol(-1) s(-1). The results are discussed in the light of recent observations on the multiphoton-excited photochemistry of serotonin.

5-Hydroxytryptophan↗

PubMed2005

Subsurface probing in diffusely scattering media using spatially offset Raman spectroscopy.

We describe a simple methodology for the effective retrieval of Raman spectra of subsurface layers in diffusely scattering media. The technique is based on the collection of Raman scattered light from surface regions that are laterally offset away from the excitation laser spot on the sample. The Raman spectra obtained in this way exhibit a variation in relative spectral intensities of the surface and subsurface layers of the sample being investigated. The data set is processed using a multivariate data analysis to yield pure Raman spectra of the individual sample layers, providing a method for the effective elimination of surface Raman scatter. The methodology is applicable to the retrieval of pure Raman spectra from depths well in excess of those accessible with conventional confocal microscopy. In this first feasibility study we have differentiated between surface and subsurface Raman signals within a diffusely scattering sample composed of two layers: trans-stilbene powder beneath a 1 mm thick over-layer of PMMA (poly(methyl methacrylate)) powder. The improvement in contrast of the subsurface trans-stilbene layer without numerical processing was 19 times. The potential applications include biomedical subsurface probing of specific tissues through different overlying tissues such as assessment of bone quality through skin, providing an effective noninvasive means of screening for bone degeneration, other skeletal disease diagnosis, and dermatology studies, as well as materials and catalyst research.

Feasibility Studies↗

PubMed2005

Numerical simulations of subsurface probing in diffusely scattering media using spatially offset Raman spectroscopy.

We present the first elementary model predicting how Raman intensities vary for a range of experimental variables for spatially offset Raman spectroscopy (SORS), a recently proposed technique for the effective retrieval of Raman spectra of subsurface layers in diffusely scattering media. The model was able to reproduce the key observations made from the first SORS experiments, namely the dependence of Raman signal intensities on the spatial offset between the illumination and collection points and the relative contributions to the overall spectrum from the top layer and sub-layer. The application of the SORS concept to a three-layer system is also discussed. The model also clearly indicates that an annular geometry, rather than a point-collection geometry, which was used in the earlier experiments, would yield much improved data.

Algorithms↗

PubMed1987

Measurement of DNA damage in mammalian cells using flow cytometry.

A technique for the detection of DNA damage induced by radiation insult has been developed. Cells were lysed with a buffer containing 2 M sodium chloride to release the DNA in a supercoiled form, the nucleoid. These were stained with the DNA intercalating dye, ethidium bromide, and exposed to laser light within a flow cytometer. Scattered and fluorescent light was analyzed from the laser/nucleoid interaction following irradiation of viable cells with gamma rays. The addition of ethidium bromide to prepared nucleoids caused a reduction in scattered light due to condensation of the nucleoid. Irradiation of cells prior to nucleoid production and ethidium bromide treatment restricted this condensation and produced a dose-dependent increase in laser scatter. Nucleoids derived from human lymphocytes showed enhanced light scatter from 5 Gy, compared to Chinese hamster ovary (CHO) fibroblasts where doses above 10 Gy were required. Up to 30 Gy CHO nucleoids showed a dose-dependent reduction in the ethidium bromide fluorescence. This technique allows detection of altered light scattering and fluorescent behavior of nucleoids after cellular irradiation; these may be related to structural changes within the nucleus induced by the radiation. The use of flow cytometry compared to other methods allows a rapid analysis of nuclear damage within individual cells.

Animals↗

PubMed1982

Forces in the metacarpophalangeal joint due to elevated fluid pressure--analysis, measurements and relevance to ulnar drift.

The mechanics of the metacarpophalangeal joint are analyzed, with a view to understanding why the finger moves in the direction of ulnar drift when the intra-articular pressure in the joint is increased. The first step is the in situ measurement of stiffness of the various tissues surrounding the joint, and the data show that (a) the two collateral ligaments are the major component and (b) the ulnar ligament is the stiffer. This latter result, combined with finding the centre of pressure at the base of the phalanx, reveals how increased intra-articular pressure produces ulnar deviation. Flexion, the other component of ulnar drift, is produced because of the volar side attachment of the ligaments. The mechanics are evaluated quantitatively by the measurement of forces at the finger tip for various pressures; the data agree reasonably well with predictions using the measured joint properties in a simplified mechanical model of the joint.

Biomechanical Phenomena↗

PubMed1978

Intra-articular pressure as a factor in initiating ulnar drift.

To determine the possible relevance of intra-articular pressure in the production of ulnar drift, metacarpophalangeal joints of fresh cadaver specimens were injected with water to increase the intra-articular pressure. Of the thirty-two joints tested, ulnar deviation was produced in twenty-three, flexion in twenty-four, and simultaneous ulnar deviation and flexion (ulnar drift) in nineteen. In two or three instances, the displacement was in extension or radial deviation. These results suggest that elevated intra-articular pressure may be a factor in the initiation of ulnar drift in patients with rheumatoid arthritis.

Aged↗