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Biomedical subjects

I P Shabalova

Publications and source records attributed to I P Shabalova.

At least 19 recordsLinked to original sources
PubMed2006

Early integration of high copy HPV16 detectable in women with normal and low grade cervical cytology and histology.

BACKGROUND: Integration of human papillomavirus (HPV) DNA has been considered a late event in cervical carcinogenesis. However, integrated forms of HPV were recently detected in cancer precursor lesions using a new real time polymerase chain reaction (PCR) to detect the deletions at the 3362-3443 region of HPV16 E2 OBJECTIVE: To study the frequency of HPV16 DNA integration in cervical lesions and compare the sensitivity of an additional upstream region of the E2 ORF (2962-3138) in detecting HPV integration. METHODS: Using the TaqMan based PCR, HPV16 positive DNA samples were analysed in 164 cervical scrapings from women participating in a multicentre screening trial. Biopsy confirmation was available in 62 cases. RESULTS: Primers targeting the 3362-3443 region detected the majority of E2 deletions. In only 23% of the samples was the E2 upstream region equal or better target than the 3362-3443 region. Mixed (episomal/integrated) pattern was the most prevalent physical state of HPV16, also present in PAP smears with normal morphology. Pure integrated form was most prevalent in HSIL and cancer lesions, but also detectable in low grade abnormalities (NSIL, ASC-US, LSIL). Women with only integrated HPV16 were almost 10 years older than those with episomal HPV16. Viral load of integrated HPV16 was related to cytological abnormality (p = 0.003) but not to histology. CONCLUSIONS: Integrated HPV16 is present in low grade cervical lesions, mostly mixed with the episomal form. Women with the pure integrated form of HPV16 are older than those with the other forms.

Adolescent↗
PubMed2005

Clearance of high-risk human papillomavirus (HPV) DNA and PAP smear abnormalities in a cohort of women subjected to HPV screening in the New Independent States of the former Soviet Union (the NIS cohort study).

BACKGROUND: We analysed the temporal relationships of the clearance of human papillomavirus (HPV) DNA and cytological abnormalities in women participating in a screening study in three NIS countries. METHODS: The 274 patients included in this analysis were prospectively followed-up for 21.6 months (range: 0.5-42.9). All 274 women had abnormal PAP test (ASC-US or higher) and high-risk HPV-positive test (HCII) at baseline. Two groups were compared: 132 women who cleared both tests (Group 1), and 142 women who cleared either HPV or abnormal PAP test (Group 2). The first clearance during the follow-up, and the last visit clearance were modeled using life-table techniques, and the predictive factors were analysed using univariate (Kaplan-Meier) and multivariate (Cox) survival analysis. RESULTS: There was no difference in the mean clearance time for the abnormal PAP test (14.4 months; 0.7-40.5 and 12.6 months; 0.5-35.0) and high-risk HPV DNA (12.67 months; 0.6-33.5 and 10.8 months; 0.7-33.4) in Group 1 and Group 2 (Mann-Whitney: P = 0.107 and P = 0.082, respectively). Clearance times for HPV DNA and abnormal PAP test did not deviate from each other in either groups (Wilcoxon: P = 0.063 and P = 0.088). The monthly clearance rates for the abnormal PAP test are 1.32 and 1.38%, and those for the HPV DNA 1.62 and 1.61%, in Groups 1 and 2, respectively. Of the factors predicting the last visit clearance, the issues related to smoking are of particular interest. CONCLUSIONS: The clearance of high-risk HPV type and abnormal PAP test shows a close temporal relationship, the former preceding the latter, however, by an interval of 1.0-2.0 months.

Analysis of Variance↗
PubMed2004

[Ploidic diagnosis of precancer processes and of uterine cervix cancer according to cytological parameters].

It is suggested, on the basis of ploidic, to define more precisely the cytological analysis according to values of the mean ploidy of cell nuclei of epitheliocytes in neoplasms of the cervix of the uterus. Ploidiometry was found to ensure a more accurate differential diagnosis of 3 process stages, i.e. benign (low degree of intraepithelial neoplasia), marginal (high degree of intraepithelial neoplasia) and malignant stages of carcinogenesis (carcinoma) in the cervix of the uterus.

Carcinoma in Situ↗
PubMed1999

[Formalized cytological report in oncology].

A formalised report (FR) offers a set of standard phrases to be used to state (1) whether findings of a cytologic study contain new information; (2) the degree of certainty in understanding the significance of morphological forms of available international classifications of a pathology said morphological evidence is compatible with. The use of that FR provides a means of evaluating reported cytologic conclusions, and comparing them with relevant postmortem diagnosis. This makes it possible to ascertain with greater or less certainty, with which nosological forms a doctor deals. It is also a means of inspection over the way materials are sampled and processed at a particular cytological laboratory as well as evaluation of its general standards. The main advantage of introduction of FR is that it is unambiguous and can be computer processed.

Cell Biology↗
PubMed1999

[Feasibilities of cytological diagnosis in the static telepathology mode].

Results of cytological diagnosis in the static telepathology mode are analyzed. Three expert cytologists made the diagnosis on the basis of images of cytological preparations obtained from 94 patients with tumors of soft tissues and bones. A total of 113 diagnoses were correct: benign or malignant type of the tumor and its histological type were identified. In general, a total of 152 (88.9%) diagnoses were correct as regards their significance for treatment strategy. Errors classified as groups 3 and 4 were the most significant (erroneous assessment of tumor type--benign or malignant--in 6.4% cases). Analysis of discrepancies between cytological and pathoanatomical diagnoses and data of routine optic microscopy of similar cytological preparations of the same material (bone and soft tissue tumors) showed the same value: 6.8% of cytological and pathoanatomical diagnoses were at disagreement (groups 3 and 4 of errors). Therefore, telepathology can be resorted to for consultations and discussion of obscure cases. It is particularly important for a morphologist to discuss complex cases by means of telepathology at a specialized institution, distant from him or her.

Cytodiagnosis↗
PubMed1998

[Use of leukocytic serum in treatment of shin trophic ulcers with protracted healing].

The results of treatment of 85 patients with trophic ulcers of various origin by leukocytic serum were compared with the results of treatment of 46 patients treated by routine method. The best clinical and aesthetic effect of this treatment was revealed in a group of patients with trophic ulcers due to vein varicosity. From 58 patients epithelization was detected in 49 (84,5%). The relapses of trophic ulcers in a period from 6 months to 1,5 years were revealed in 5 patients. The use of leukocytic serum ensures high percent of closure of the ulcers chiefly of venous etiology.

Adult↗
PubMed1997

Phyllodes tumour: cytologic and histologic presentation of 22 cases, and immunohistochemical demonstration of p53.

A retrospective study of 22 cases of phyllodes tumour (PT) was undertaken to evaluate the potential value of fine needle aspiration (FNA) cytology in the diagnosis of benign and borderline PT. Histological material was available from 12 patients with typical benign PT (group 1), six patients with less typical changes (group 2) and four cases of borderline PT (group 3). Cytological presentation of PT in these cases was similar to that described by other cytologists, although abundant cellular material was obtained in only eight FNAs, naked nuclei were present in nine cases only, and atypical or suspicious cytological features were found in seven cases. Comparative analysis of p53 was made in nine patients with PT, five cases with other benign breast lesions and five with malignant lesions. p53 reaction was positive in five of nine patients with PT (all cases from groups 2 and 3), compared with two of five cases of carcinoma. p53 was negative in all patients with PT from group 1 and the five other benign cases. We suggest that cytopathologists should be careful when a myxoid stromal component is present in cytological smears.

Adult↗
PubMed1991

[Cytologic diagnosis of malignant tumors of the cervix].

Cytologic patterns of squamous-cell carcinoma, adenocarcinoma, malignant adenoma, and carcinoid of the cervix uteri are presented. Problems in the differential diagnosis of these conditions are discussed. Special attention is paid to the cytologic criteria for detection of intraepithelial and microinvasive squamous-cell and glandular carcinomas. Cytologic investigation may essentially contribute to timely diagnosis of the condition.

Adenocarcinoma↗
PubMed1990

[The examination of vaginal discharge].

Discusses basic principles of hormonal cytodiagnosis. Emphasizes the role of the hypothalamohypophyseal system in regulation of sexual gland function and ovarian effect on vaginal epithelium maturation in various phases of the menstrual cycle. Shows potentialities of hormonal profile assessment on the basis of various indices and the total cytologic picture. Characterizes the mechanisms that provide epithelial resistance to pathogenic factors. Pays special attention to cellular composition of smears in inflammatory diseases induced by pathogenic fungi, protozoa, bacteria, viruses.

Female↗
PubMed1990

[Cytologic diagnosis of background and precancerous conditions based on specimens from the uterine cervix].

The authors emphasize the value of cytologic diagnosis in the detection and treatment of various conditions that may be regarded as direct or indirect precursors of carcinoma of the cervix uteri. Conditions for obtaining and treatment of the material for high-quality cytologic analysis are described. Potentialities and clinical value of the cytologic diagnosis are discussed, as are specific features of the cytograms in squamous-cell metaplasia, endocervicitis, leukoplakia, virus-induced changes in the epithelium, and dysplasia of various severity. The authors point to the important role of cytologic diagnosis in examinations of women with apparent gynecologic diseases and without manifest clinical or visual changes in the cervix uteri.

Cervix Uteri↗
PubMed1990

[The use of immunochemical reactions in determining a cytological diagnosis].

Cytologic preparations of 56 samples of pleural fluid, of 31 samples of ascitic fluid, and 13 puncture biopsy specimens were examined in immunocytochemical tests. Carcinoembryonic antigen and trophoblastic beta 2-globulin were detected in the cytograms. Antibodies to carcinoembryonic antigen and trophoblastic beta 2-globulin employed in the tests did not react with the cells from benign exudation, because tumor cells, particularly glandular carcinoma ones, contain these markers in high concentrations.

Ascitic Fluid↗
PubMed1990

[Antigenic markers in the cytological diagnosis of human malignant neoplasms].

The review deals with antigenic markers used for immunocytochemical diagnosis of malignant tumours describes theoretical and practical basis for identification of malignant cells in lymph nodes, bone marrow, peritoneal and pleural effusions and sputum with application of tumour markers. The identification of micrometastases in lymph nodes of patients with gastric cancer and the identification of malignant cells in smears from effusions illustrate the authors' own results. The description of immunocytochemical methods is given.

Antibodies, Monoclonal↗