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Biomedical subjects

I Paskaleva

Publications and source records attributed to I Paskaleva.

11 recordsLinked to original sources
PubMed1999

Persistence and in vivo effects of Yersinia enterocolitica 0:3 endotoxin in rats.

In vivo effects of Yersinia enterocolitica 0:3 lipopolysaccharide (prepared from bacteria grown at 25 degrees C and 37 degrees C) were investigated after intraperitoneal (i.p.) and intraarticular (i.a.) injection in rats during 30 days of examination. The persistence of endotoxin in the peritoneal and the synovial cavities was demonstrated by the immunofluorescence technique. Peritoneal and synovial exudative cell infiltration, as well as changes in some parameters (glycolytic and acid phosphatase activity, and killing ability of peritoneal cells; lactate dehydrogenase concentration in synovial fluid) were studied. The results indicated that endotoxin could persist longer in the synovial than in the peritoneal cavity.

Animals↗
PubMed1999

The fate of Yersinia pseudotuberculosis lipopolysaccharide (LPS) in intraperitoneally and intraarticularly infected rats.

Persistence and in vivo effects of Yersinia pseudotuberculosis serotype III LPS (prepared from bacteria grown at 25 degrees and 37 degrees C) in rats were investigated after intraperitoneal and intraarticular injection during the 30 day period of examination. Localization and persistence of LPS in the peritoneal and synovial cavities were demonstrated by using the immunofluorescence technique. Peritoneal and synovial exudative cell infiltration as well as changes in some parameters (glycolytic and acid phosphatase activities, killing ability of peritoneal cells, lactate-dehydrogenase concentration in synovial fluid) were studied. The results indicated that LPS expressed at 37 degrees C induced stronger peritoneal cell response (increased "killing" ability and elevation of glycolytic and acid phosphatase activities) in comparison to that synthesized at 25 degrees C. It was also found, that LPS persisted longer in the synovial rather than in the peritoneal cavity of rats and induced long-lasting synovial inflammation.

Animals↗
PubMed1997

Cell wall-deficient forms (L-forms) of Listeria monocytogenes in experimentally infected rats.

Experimental infections were induced with different bacterial forms of Listeria monocytogenes: parental (S-forms), protoplastic (L-forms) and combined inoculum of both forms by i.p. injection of rats. The parental bacterial forms (S-forms) were isolated up to 7 days after challenge from the peritoneal cavity and the liver, while the L-forms were isolated up to 60 days from the peritoneal cavity. Continuous adhesion of L-forms on the peritoneal macrophage surface was found by scanning-electron microscopy. Erythrocyte and leucocyte count as well as some clinical chemistry parameters were measured during infections. They showed different dynamics in the three experimental groups. Histomorphological changes in the liver (microabscesses and mononuclear cellular granulomas) of infected animals were observed. They were less intensive and appeared later in rats infected with L-forms. The experiments demonstrated that infections caused by parental bacterial forms and by combined inoculum took an acute course, while the infection caused by L-forms could be distinguished as a prolonged and persistent one.

Animals↗
PubMed1997

Influence of blood transfusion on bactericidal activity of human leukocytes and sera against Yersinia enterocolitica and Salmonella typhimurium.

Patients undergoing joint surgery and blood transfusion were studied. Serum and leukocyte bactericidal tests in vitro against Salmonella typhimurium and Yersinia enterocolitica were carried out preoperatively as well as on the 1st, 3rd and 7th days after the operation. The serum complement (C3 and C4) concentrations were determined at the same intervals. It was found that after blood transfusion the bactericidic activity of sera and the serum C3 complement concentrations were increased. In contrast the killing ability of leukocytes was suppressed.

Blood Bactericidal Activity↗
PubMed1986

[Acid lysosomal hydrolase activity in the serum and synovial fluid of rheumatoid arthritis patients].

Five acid lysosome hydrolases were studied in 20 patients with rheumatoid arthritis (RA), active stage in serum (S) and synovial fluid (SF) in parallel, obtained from the knee joint. The enzyme activity was fluorimetrically determined, making use of substrates specific for each enzyme, derivatives of 4-methyl-umbelliferone. The serum lysosome activity of the patients with RA, compared with that of a referent group of healthy subjects, showed a significant increase in three of the hydrolases studied. With the juxtaposition of enzyme activity between SF and serum of the patients with RA, the activity of all acid hydrolases in the synovial fluid studied proved to be several times higher and with high statistical significance (p less than 0.01-0.001). Our data support the valuable informative value of the study on acid lysosome hydrolases in the synovial fluid. A sensitive indicator of the activity of the joint process in RA is the coefficient of lysosome enzymatic activity SF/S, substantially surpassing 1.0.

Adolescent↗
PubMed1985

[Bactericidal effect of the preparation trozin].

Tested was the bactericidal action of the combined cleansing and disinfecting preparation trozin under laboratory and production conditions. It was found that trozin meets the requirements in terms of rapid disinfection, killing the asporogenous test organisms. The presence of at least 2 per cent milk protein lowers the bactericidal action of the detergent. The cleansing and disinfection of milking units supplied with a milk-can, resp., with a central milk pipeline leads to 97.00, resp., 98.00 per cent reduction, on an average, of microflora. The bacterial count of the udder is likewise reduced by about 96.00 per cent.

Animals↗
PubMed1981

[Dynamics of lysosomal enzymes in patients with allergic dermatoses before and after high altitude climate therapy].

Lysosome enzymes were measured in patients with allergic dermatosis before and after high altitude climate therapy. The activity of five acid lysosomal hydrolases in the serum of patients with allergic dermatoses before and after 30 days were investigated. In all cases a reduction of the activity of alpha-glucosidase was observed. A similar reduction of the activity of beta-glucuronidase and beta-galactosidase was observed.

Adult↗