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Biomedical subjects

I Peshkov

Publications and source records attributed to I Peshkov.

7 recordsLinked to original sources
PubMed1979

[Diagnostic titers in neorickettsiosis in sheep, cattle and swine].

Investigations were carried out after a micromethod for RCB formulation and standardization proposed by the authors used as the basic method of neorickettsiosis diagnositcs. In the course of 4 years 3614 serums of healthy animals and 289 serums of sheep, cattle and swine suffering from neorickettsiosis were investigated. It was established that the titre of complement binding antibodies in neorickettsiosis is stable. Variations are rarely observed and they are of low titre grade. Specific neorickettsiosis titres in sheep, cattle and swine are considerably high (1:128 to 1:256 and 1:512). The diagnostic value of low titres is in correlation with the phase of the infection process development and can be assessed only by following the dynamics of complement binding antibodies.

Animals↗
PubMed1979

[Antigenic structure of the L forms of Br. Abortus 544].

The gel precipitation test after Ouchterlony and Mancini was employed to study comparatively the antigenic structures of S and L forms of Brucella abortus 544. Reduction of the antigenic fractions was established with the L-transformed Brucella organisms as compared to the initial cultures. Reduced were mainly fractions of the antigenic structures that took part in cell wall building. Cytoplasmic antigens remained unchanged. Those that participated in the building of cell walls precipitated in compact lines. On the other hand, the cytoplasmic antigens produced diffuse-and-compact precipitation zones. The intact antigenic structures retained their specificity.

Animals↗
PubMed1978

[Diagnostic value of agglutination reaction with buffered Brucella antigen stained with rose bengal].

A specific buffered antigen has been obtained, employing a method developed by the authors, stained with Bengal rose and intended for performing a fast agglutination reaction to confirm brucellosis. The antigen produces a clear and demonstrative agglutination reaction with positive sera. Practically, the test is readily carried out, and can be made a routine both in every serologic laboratory and for investigations under field conditions. The reaction produced with this antigen can determine dependably the epizootic status on a farm or in the herd. The diagnostic value of the antigen is essential, especially with swine. Besides, it shows a wide a diagnostic scope for brucellosis with all species of animals (97--98 per cent).

Agglutination Tests↗
PubMed1978

[Experiments for the serological establishment of the species of mycobacteria inducing an immune restructuring of the macroorganism].

Serological investigations are carried out on hyperimmune rabbit and guinea pig sera with two antigens each of Mycobacterium tuberculosis H37Rv, Mycobacterium bovinus, Mycobacterium avium and Mycobacterium Kanzassi. One of the antigens is obtained through electrohydraulic destruction of delipidated mycobacteria, while the other constitutes cell walls, purified with ribonuclease, of non-delipidated mycobacterial cells, destroyed by the same method. The antigens, prepared by the electrohydraulic method, possess a markedly expressed complement-fixing capacity, conversely to the antigen from cell walls, which is slightly active in complement-fixation tests. Again a trend is observed towards a possible serological indentification of the species of mycobacteria that have caused the immune transformation of the major organism; yet the questions still remain to be settled. The immunogenes used possess a high activity and induce high titre antisera, established also in other investigations, which make reasonable their study as vaccines.

Absorption↗
PubMed1978

[Aspects related to the antigenic structure and serological specificity of brucellae phylogenetically related to S- and R-forms and dissociated into R-variants].

The antigenic structures and serological properties of Brucellae S- and R-forms, phylogenetically differentiated, and dissociated R-variants are studied by means of gel-precipitation, immunoelectrophoresis and fixation of the complement. The Brucella suis 1330S, Brucella suis 1330R, Brucella ovis 02 and Brucella abortus 99 are involved in the experiments. Both specific and general antigenic structures are established in all strains studied, but only homologous antibodies from the antigen-antibody complex in the complement-fixation test, induced by the phylogenetically differentiated S- and R-forms (Brucella ovis) or brucellae, diverged into R-variants. Cross complement-fixation tests between the antisera, prepared against S-forms and antigens from natural R-forms (Brucella ovis) or dissociative R-forms (R-variants) have not been observed. Also cross reactions between antisera, induced by R-forms (natural R-forms--Brucella ovis, or dissociative R-variants) and antigens, obtained from S-forms of brucellae are found.

Antigen-Antibody Complex↗
PubMed1977

[Differentiation of non-specific positive brucellosis reactions using an antigen stained with rose bengal].

A Brucella buffered antigen, stained with Bengal rose by a method of the authors, was obtained. It showed a high distinguishing capacity with regard to the nonspecific agglutinations after Huddleson and Wright at a negative complement-fixation test for brucellosis with sera from cattle, pigs, sheep, and horses. Such differentiation, however, proved to be incomplete and for sera of different animals varied within the range of 34 to 0.0 per cent.

Agglutination Tests↗