The concentration of macromolecules by electrophoresis-sedimentation.
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Biomedical subjects
Publications and source records attributed to I Posner.
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A simple and specific method for assaying lipoprotein lipase activity is described. Postheparin plasma, heart homogenates, or extracts of acetone powder of adipose tissue were incubated with a triolein-coated Celite substrate, and enzyme activity was determined from the rate of free fatty acid (FFA) release in the incubation system. FFA release was linear for 30 min, and was proportional to protein concentration in the incubation system. FFA release was decreased by addition of deoxycholate or Triton X-100. Increasing the concentration of heparin in the incubation system caused a gradual decrease in FFA release by postheparin plasma and increases in activity of heart homogenates and adipose tissue lipoprotein lipase. The Celite substrate was found to be satisfactory for assaying pancreatic lipase activity as well.
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The overall goal of Stress Management Training is the re-orientation of people's typical stress reaction habits into new, more rational and assertive patterns of problem-solving. Participants gain new insights into what stress is and how it affects them. They learn skills which reduce stress to nondamaging levels. They learn to use their minds to productively solve problems and to manage their personal and professional lives to a better end.
Traditional methods of bone mineral analysis were compared to a new technique using computerized tomography (or CT scanning). The correlation between the CT values for long bones in the dog and actual calcium content was 0.9942. The correlation between two photon absorptiometric techniques and CT values were also 0.9981 and 0.9962. The precision of repeated scans was +/-2%. It is suggested that the CT scanner will allow bone mineral analysis to be performed with accuracy and precision.
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