Biomedical subjects
Publications and source records attributed to I Pousty.
A fish, Sparus datnia (Ham. Buch.), Sparidae, protandric, is one of the species, which has a diffusing intrahepatic pancreas. The gland is compound of two parts : the exocrine part (follow the branches of the portal vein), and the endocrine part (langerhans islets, more or less abundent in the external side of the endocrine part). Three kinds of granular cells are recognized in the endocrine islets.
Intervertebral discs of six species were stained with Alcian blue and PAS methods to identify glycosaminoglycans. The polyanions are most concentrated in 'ring-like' pericellular areas and in material lying between individual lamellae and bundles of collagen. A slight increase occurs early in life and towards the disc centre. The concentration of glycosaminoglycans cannot be correlated with species which are known to suffer from clinical disc disease, though there appears to be a higher concentration in the discs of the cat and dog than in other species studied.
Disc material from horse, ox, sheep, pig, dog and cat was stained by the Alcian-blue-critical electrolyte concentration technique and with the standard and two-step periodic acid Schiff methods. The effects of pretreatment with hyaluronidase and with chondroitinase was also evaluated. There appears to be a small increase in total cellular glycosaminoglycan content with age in all species: cellular material of high molecular weight however only increases in aged animals. The degree of sulphation of cellular glycosaminoglycans does not vary with age or with position in the disc.
Intervertebral discs of an old sheep and a young pig were examined for the presence of cells containing the enzyme uridine diphosphoglucose dehydrogenase. In the sheep, the inner anulus had a higher proportion of active cells than the outer anulus; in the pig, there was no difference. From a consideration of cell numbers, it is suggested that there is an accumulation of glycosaminoglycans in the centre of the disc rather than an increased production rate. Notochordal cells in the pig disc contain uridine diphosphoglucose dehydrogenase and are capable of producing glycosaminoglycans.
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