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Biomedical subjects

I Reimann

Publications and source records attributed to I Reimann.

At least 19 recordsLinked to original sources
PubMed1994

Effect of adjuvant formulations on the induction of virus-neutralizing and virus-binding antibodies by chemosynthetic peptides of VP1 of foot-and-mouth-disease virus.

Synthetic peptides corresponding to the 141-160 amino acid sequence of the protein VP1 of virus type O1 Kaufbeuren (O1K) and A5 Riems (A5R) were conjugated to thyroglobulin and mixed with complete Freund's adjuvant (CFA) or incomplete Freund's adjuvant (IFA) together with Quil A. Although the peptide of A5R, together with IFA and Quil A, or with CFA, elicited a high antibody response to the virus in the ELISA, formula containing both IFA and Quil A induced only high titres of virus-neutralizing antibodies in rabbits. These adjuvant formulas did not display significant differences with regard to side effects.

Adjuvants, Immunologic↗
PubMed1994

Bone banking in Denmark, results of a nationwide survey.

In a nationwide survey, all orthopaedic clinics were surveyed via a questionnaire about the use of bone allografts and how they managed their bone banks. Thirty-two clinics (100 per cent) responded to the questionnaire (mid 1991). Seventeen clinics had established bone banks on the basis of femoral heads obtained from donors during primary hip replacement. Only five used bone substitutes. The mean consumption was 30 capita per year (10-132) and ten clinics estimated an increasing demand for allografts. The storage method was by freezing at temperatures varying from minus 20 to minus 80 degrees Celsius. Contraindications to procurement comprised history of infection and malignancy, all clinics tested donors for HIV antibodies and all but one for hepatitis B. Testing for hepatitis C was about to be introduced. All but one clinic developed cultures from the procured bone. Informed consent was employed by nearly all clinics, but very few obtained written consent. Since this survey, revised recommendations have been directed from the Danish National Board of Health, these listing that an HIV-test should be performed with a 90-day interval, and that testing should also be done for hepatitis B and C. International experience and this survey show that the establishment of more specific and general national recommendations would be preferable to present practice.

Bone Banks↗
PubMed1993

[The pharmacokinetics of a new anti-arrhythmic substance, Z-2-amino-5-chlorobenzophenoneamidinohydrazone (AWD-G256)].

In 4 studies blood levels and metabolites of Z-2-amino-5-chlorobenzophenoneamidinohydrazoneacetate (AWD-G256) after different dosages and forms of application on patients and healthy volunteers were investigated. In addition the pharmacokinetic parameters were calculated. The determination of pharmacokinetic parameters was possible only after i.v. application of dosages higher than 0.30 mg.kg-1 b.m. as well as p.o. administration of dosages higher than 100 mg. The blood level curve after i.v. application demonstrated a 2-compartment model and after p.o. application a 1-compartment model. Exact pharmacokinetic data can't be clear estimated with this studies.

Administration, Oral↗
PubMed1993

[Clinical-electrophysiologic studies on the actions of the anti-arrhythmic substance AWD-G256 in man].

The effects of the new antiarrhythmic drug AWD-G256 (1) were investigated by clinical electrophysiology (His bundle electrography, programmed electrical stimulation) in 19 patients with supraventricular tachycardias but without structural heart disease. In a maximal dosage of 0.45 mg/kg body mass 1 only minimally affects electrophysiological parameters of the impulse formation and conduction. At this time the therapeutic value of 1 is not clear.

Adult↗
PubMed1993

[Hemodynamic effects and adverse effects of the anti-arrhythmic substance AWD-G256 in man].

In 19 patients with different supraventricular tachycardias the antiarrhythmic drug AWD-G256 was studied to investigate the effects on hemodynamic parameters. Over all, stroke volume, pulmonary pressure and systemic blood pressure were not significantly altered. The only main adverse effect was a transient rise of serum transaminases in two patients. We conclude that AWD-G256 is usually hemodynamically tolerated, but the antiarrhythmically effective dosage is not reached yet.

Adult↗
PubMed1992

Drug use in pregnancy: east German data of an international collaborative study.

In an epidemiological collaborative international study under the auspices of WHO-Euro the drug use in pregnancy was investigated in 22 countries in 14,778 women and in hospitals of three different levels. The East German state Thuringia took part according to the annual birth rate with 300 women in 3 hospitals (Jena, Gera, Greiz). In the international average, 14% of the enrolled women took no drugs during pregnancy before admission to hospital for delivery. In East Germany 44% of the women taking part in the study had no drug administration during pregnancy. The critical use of iron preparations and vitamins there has to be mentioned. Under delivery, 99% of the Thuringian women received drugs. In the puerperium the administration of ergot derivatives and of laxatives exceeds the international average and has to be critically revised, but the administration of antiinfectives in only 1/5 and of analgetics 1/3 of the international average.

Drug Utilization↗
PubMed1992

Investigations into a new antiarrhythmic substance Z-2-amino-5-chlor-benzophenon-amidin-hydralazin in humans.

Z-2-amino-5-chlor-benzophenon-amidin-hydralazin is a new chemical potential antiarrhythmic substance. The blood levels were investigated after different dosages and forms of administration in patients and volunteers. The determination of the substance can be performed by high-pressure liquid chromatography. In addition the pharmacokinetic parameters were calculated. The pharmacokinetic profile is similar in humans and animals.

Administration, Oral↗
PubMed1991

Resorption of intraarticular diffusible and microcolloid tracers. Rabbit studies of normal and synovitic knees.

The resorption of two radiotracers (99mtechnetium-labeled microcolloid particles to study lymphatic transport and 51 chromium-EDTA to study diffusion) from the knee joint and the subcutaneous tissue of rabbits was investigated simultaneously. In 12 rabbits, synovitis was induced in the right knee 6 weeks or 3 months before the investigation; 6 rabbits served as controls. The final number of Tc-particles in the normal knees and in the subcutaneous tissue in the three groups did not differ; but removal from the synovitic knees was increased. The final number of Cr-EDTA particles did not differ within or between groups. The initial decrease was highest in the knees with acute synovitis (P less than 0.05). The results indicate (1) that in synovial tissue lymphatic transport is of little importance, (2) that leakage through the synovial membrane increases in synovitis, and (3) that a subcutaneous depot can be used as a reference instead of injections into a normal knee.

Animals↗
PubMed1991

Articular cartilage changes following meniscal lesions. Repair and meniscectomy studied in the rabbit knee.

A well-defined, longitudinal lesion in the avascular part of the medial meniscus of the right knee was made in 30 rabbits. After 3 months, the lesion was repaired surgically in 12 rabbits, was untreated in 12 rabbits, and a meniscectomy was performed in 6 rabbits. The articular cartilage was studied macroscopically and microscopically at 3-month intervals. Cartilage changes 3 months after meniscectomy were more pronounced than after meniscal repair or than in untreated lesions. However, meniscal repair did not reverse the cartilage changes.

Animals↗
PubMed1991

Repair of three-month-old experimental meniscal lesions in rabbits.

Repair of three-month-old longitudinal meniscal lesions in the central avascular portion of the knee joint was investigated in 18 rabbits. Three months after a longitudinal incision was made in the avascular portion of the meniscus, no healing was observed. At that time a full-thickness radial cut from the lesion to the joint capsule, without using synovial flaps or implants, was performed in ten of the 18 rabbits. Three months later, nine of the ten menisci had healed mainly with cartilaginous tissue, although the repaired areas were different from the normal fibrocartilage both histologically and at gross inspection. Synovitis with hyperplasia of the lining cells, which was present in all cases at the time of repair, may play an important role in the healing process.

Animals↗
PubMed1990

[Chemosynthetic peptides against foot-and-mouth disease--immune response to free and carrier bound peptides of the VP1 of O1-Kaufbeuren].

Three peptides of main epitope of FMD virus O1-Kaufbeuren, VP1 (16, 21, 31), were found to induce in the 130-160 sequence range, in free and/or carrier-bonded form, virus-neutralising antibodies in guinea pig, rabbit, mouse, swine, and cattle. Five carrier proteins were tested, with thyroglobulin, next to keyhole limpet hemocyanin (KLH), being most effective for 16-peptides (145-160) and 21-peptides (141-160 Tyr161). To protect guinea pig from FMD, minimum dosage of 21-peptide was found to be 2 x 8 micrograms. The immunogenic spectrum of peptides and conjugates proved to be broader than that of monovalent vaccines of inactivated virus. Free peptides were found to be also capable in vitro of inhibiting virus infection.

Animals↗
PubMed1990

Synthetic peptides against foot-and-mouth disease--immunization with VP1-peptides of type O1-Kaufbeuren.

Coupled synthetic peptides, representing the sequences of amino acids 130-160, 141-160 and 145-160 of foot-and-mouth disease virus O1K protein VP1, induced virus-binding and virus-neutralizing antibody response in guinea pigs, rabbits, and pigs. We also detected antibody response in guinea pigs after immunization with uncoupled peptides and in cattle with 21 aa-peptide-Keyhole-limpet hemocyanin (-KLH). The best results were obtained from 21 aa-peptide-KLH and 31 aa-peptide with or without KLH or thyroglobulin as carrier. Our preliminary results show the induction of virus-neutralizing antibodies to be obviously influenced by length of the peptide as well as by the kind of carrier and coupling.

Animals↗
PubMed1989

Measurements of pressure on the sole of the foot in plaster of Paris casts on the lower leg.

Pressure on the sole of the foot inside three different types of plaster cast, used in treatment of fracture of the lower leg, was measured on six normal persons. No significant difference was found between these pressures in below-knee plaster, full length plaster including the thigh and patellar-tendon-bearing plaster. Only occasionally a relief in pressure was found in patellar-tendon-bearing plasters.

Casts, Surgical↗
PubMed1989

Lymphatic transport from normal and synovitic knees in rabbits.

The resorption pattern of synovial fluid through the lymphatic system from normal and synovitic knee joints in rabbits was studied with 99mtechnetium-rhenium-sulfur colloid injected intraarticularly and monitored for 14 hours with a gamma camera. On the normal side the regional lymph nodes were visualized after 1 hour and after 14 hours still 75 percent activity remained in the knee. In the synovitic knees no lymphatic transport could be detected; and the radiotracer was unstable with rapid liberation of technetium, which was excreted in the urine. This radiolysis was not found in vitro in synovitic joint fluid. The lymphatic transport from normal rabbit knees is low. We found a clear difference in lymphatic transport between normal and synovitic knee joints.

Animals↗
PubMed1989

Sonography in soft-tissue trauma of the shoulder.

Seventy-five patients with soft-tissue trauma in the shoulder region were examined by ultrasonography. Normal conditions were found in 21 patients, distension of the acromioclavicular joint in 17, muscle hematoma or edema in 9, biceps tendinitis in 8, hematoma or partial rupture of the biceps tendon in 5, rupture of the biceps tendon in 1, and supraspinatus tendinitis or partial rupture in 14. A correlation was found between the sonography and clinical course; normal sonography was consistent with recovery in less than 1 week, whereas patients with pathologic changes in the supraspinatus or biceps tendon generally had symptoms for at least 1 month. We concluded that sonography is a useful diagnostic tool for evaluation of soft-tissue trauma in the shoulder.

Adolescent↗
PubMed1989

Porous-coated knee arthroplasty. A case report concerning bone ingrowth.

The area of bone-metal interaction in uncemented implants is regarded by many investigators as the key to the success or failure of the implant. The nature of the interaction is poorly understood because the zone is technically difficult to visualize and analyze. In order to test the power of modern imaging, analyzing, and metallurgical methods in this context, a well-functioning porous-coated knee prosthesis (tibial component) removed from the knee of a 65-year-old man was sectioned and examined by light microscopy, roentgenogram, scanning electron microscopy, and radiologic energy dispersive analysis. Independently, these methods demonstrated that the prosthesis was held in situ by collagenous tissue between and below the metal pellets of the prosthesis. Calcified bone did not appear to interact with the prosthesis and is probably not a major factor for prosthesis fixation. The various analytic methods described are suitable and sufficient for further exploration on a larger scale of the zone of bone-metal contact in cementless implants.

Aged↗