A two-phase colorimetric method for the measurement of pepsin activity.
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Biomedical subjects
Publications and source records attributed to I Rosival.
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The nonspecific, stimulative effect of vitamin A, applied per os, was studied on the T and B cell population in the course of post-invasive, migratory phase of experimental ascariasis of swine. The vitamin under study was found to exert an activating influence on the level of both immunocompetent cells already in the early phase of invasive process, with the maximum on the 9th, and/or 12th day after invasion. The protective effect of vitamin A, i. e. the effect on the reduction of migrating larvae of ascarids in the lungs, was 50%. This phenomenon is in accord with the present knowledge on the role of this vitamin in immunity.
A comparative study of glutamate dehydrogenase (GLDH 1.4.1.2) and glutamine synthetase (GS 6.3.1.2.) activity in liver, kidney and spleen homogenates from cattle, sheep, pigs and chickens showed that chicken liver contained on an average 3.5%, pig liver 8.3% and bovine liver 45.6% of the glutamate dehydrogenase activity present in sheep liver. Relatively low trace activity was found in the spleen and kidneys, except for the renal cortex of cattle (32% of activity in the liver). GS activity was the highest in chicken liver; in pigs it amounted to 33.40%, in cattle to 24.2% and in sheep to 19.7% of this activity. No marked interspecies differences were found in the values in the kidneys and spleen. It can be concluded from the results that the relatively high GLDH activity in the liver of ruminants compared with pigs and chicken is associated with the greater ability of ruminants to utilize ammonia. The higher GS activity and lower GLDH activity in chicken liver can be attributed to higher uric acid synthesis from ammonia via glutamine and purine bases and the lower ability of birds to utilize ammonia for protein synthesis. The presence of alanine dehydrogenase was not demonstrated in chicken liver, where the maximum oxidation of NADH after the addition to pyruvate and ammonia substrate was found.
The total values were determined for the activity of glutamate dehydrogenase, glutamine synthetase, and dehydrogenase with pyruvate in broilers fed a diet with a 0, 2 and 4% content of urea for three weeks. A statistically significant increase of glutamate dehydrogenase activity was ascertained in the liver and kidney of broilers. The increase of the activity of glutamine synthetase in liver was close to the threshold of statistical significance. Dehydrogenase activity with pyruvate increased in liver.
Commercial feed mixture was buffered with a 2% and 3% admixture of bentonite buffer in two beef cattle herds in the course of one year. The mixtures were fed on a continuous basis. The two-per-cent buffer concentration was tested in 110 test animals with 104 control animals and the three-per-cent concentration in 50 test animals with 50 controls. Throughout the trial the over-all health condition remained unchanged, the hematocrit and hemoglobin values were balanced in both groups. The biochemical indices were better in the test groups: hypocalcemia improved (in the controls it grew worse), magnesiemia was slightly increased, the inorganic serum factor did not go beyond physiological limits, and acidosis did not occur (as distinct from the control animals). The levels of transaminases (GOT, GPT), glutamic acid dehydrogenase, total serum protein, alkaline phosphatase as well as ammonia and urea in blood serum were at physiological values with po-differences within groups. In the case of the three-per-cent buffer concentration the daily gains were higher by 0.073 kg, and in the two-per-cent concentration by 0.058 kg, in the test animals. The average annual gain was higher by 25.5 kg, and by 18.3 kg, respectively. With respect to the price of buffer and to the efficiency of the animals tested, the economic indices of feed mixture buffering are highly effective.
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