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Biomedical subjects

I S Miller

Publications and source records attributed to I S Miller.

4 recordsLinked to original sources

Experiential psychoanalysis and the engagement of selves: Ferenczi's vision and the psychoanalytic present.

Recent interest in the life and work of Sandor Ferenczi reconsiders his pivotal role within the development of clinical psychoanalysis (Nemes, 1988; Thompson, 1988). Ferenczi's contributions have been linked to therapeutic orientations that include Rogerian humanistic psychology; Kohutian self psychology; the object relational approach of D. W. Winnicott; and interpersonal psychoanalysis (Katz, 1988; Lum, 1988; Rachman, 1988). Among his analysands were such divergent psychoanalytic pioneers as Ernest Jones, Melanie Klein, Michael Balint, Sandor Rado, and Clara Thompson.

Countertransference↗

Klebsiella pneumoniae nif-lac fusions are expressed in Agrobacterium tumefaciens C58.

Plasmids containing hybrid genes, in which different Klebsiella pneumoniae nif (nitrogen-fixation) promoters were fused with the structural part of the Escherichia coli lac operon, were introduced into a double auxotrophic derivative of Agrobacterium tumefaciens C58. A study of their expression in the new host was made simple by the inherent inability of A. tumefaciens C58 to produce beta-galactosidase unless provided with the wild-type lac operon of E. coli. As shown by quantitative measurements of the enzyme, all K. pneumoniae promoters were expressed well in A. tumefaciens C58, even under conditions known to repress them. It also has been shown that the activity of K. pneumoniae nif A is essential for the expression of nifHDK even when introduced into A. tumefaciens. After entering the new host the plasmids, the nif genes and the fusion alleles contained in them, remained stable. Possible mechanisms responsible for the constitutive behaviour of nif promoters in A. tumefaciens are discussed.

Cloning, Molecular↗