SEXUAL DIMORPHISM OF MOUSE SUBMAXILLARY GLANDS AND ITS RELATIONSHIP TO NERVE GROWTH STIMULATING PROTEIN.
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to I SCHENKEIN.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Cohen's method for preparing the nerve growth factor from mouse submaxillary glands was followed to the last ammonium sulfate fraction. Further purification was accomplished with carboxymethyl- and diethylaminoethyl- column chromatography. Three peaks were obtained for each column; the third peak obtained with diethylaminoethyl cellulose was the most active. Electrophoresis of this active fraction produced one anodal and two cathodal bands. Each band was inactive for nerve outgrowth. However combinations of the cathodal bands produced 3+ growth. One cathodal band was not dialyzable; the other was dialyzable and negative for ultraviolet absorption at 280 millimicrons.