[Psychiatric Cluj. A short historical sketch].
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History, 19th Century↗
Biomedical subjects
I Samuel
Publications and source records attributed to I Samuel.
The action of dyes on tumors.
The photodynamic and antimetabolic action of dyes on experimental tumors is reviewed. The author's personal experience concerning the action of toluidine blue on mouse Ehrlich ascites carcinoma is presented. Some considerations are made on the problems and prospects of the application of dyes in antitumoral therapy.
Animals↗
Studies on the infectivity of viral nucleic acids performed in the "Stefan S. Nicolau" Institute of Virology.
The investigations on viral nucleic acids (NA) performed in the Institute of Virology since 1957 refer to the infectivity of NA (RNA or DNA) from large, complex viruses, from RNA viruses (ECHO, MM Coxsackie, etc.), from modified viruses, as well as to the biological activity (oncogenicity and infectivity) of NA from tumors and leukemic tissues of human, murine, bovine and avian origin. Studies are reviewed as regards the action of physical and chemical agents on the behaviour of viral and cellular NA and the implications of the use of such agents in viral vaccine preparation are discussed.
Animals↗
[Research in the field of the infectivity of viral nucleic acids].
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DNA, Viral↗
The effect of some drugs and of ceruloplasmin on experimental influenza APR 8/34 (H0N1) infection in the mouse.
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Aminopyrine↗
Spectrofluorimetric investigation of cell permeability in tissue cultures.
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Cell Membrane Permeability↗
Gel filtration through Sephadex G25 of virus fluorescent compound systems. Comparison with ultracentrifugation results.
A stable interaction could be made evident between influenza A(H1N1) or Sendai virus and two fluorescent naphthalic acid derivatives solubilized in DMSO. The virus-compound interaction was studied spectrophotometrically and spectrofluorometrically, at the wavelengths characteristic of virus protein and of the two compounds, in samples subjected to gel filtration or ultracentrifugation. The percentages of virus-bound compound obtained by the two methods were similar in the case of the vinyl-sulfonic derivative (affecting virus structure to a lesser extent) and considerably different in that of the isocyano derivative (causing important structural damage).
Chromatography, Gel↗
The relationship between Sendai virus envelope antigens and the immune response in the presence and absence of ceruloplasmin.
The immune response of rabbits inoculated with different Sendai virus glycoprotein antigens depends on the structural identity of the protein support as well as on the mode of organization and structural homogeneity of the solubilized virus envelope. Incubation of the antigens with ceruloplasmin leads to modifications in the immune response, owing to the interaction with the active hemagglutinating and enzymatic sites.
Animals↗
Some particularities of Sendai virus multiplication in chorioallantoic membrane fragments.
Marked differences were recorded in the kinetics of infectant and hemagglutinating (HA) activities of parinfluenza type 1 (Sendai) virus grown in chorioallantoic membrane (CAM) fragments in roller tubes with or without daily changes of the culture medium. The differences were dependent on cultivation conditions, the time interval post inoculation and the state of the CAM cells. Taking into account these conditions, it is possible to obtain either highly infectant virus or virus with low infectivity but high HA activity.
Allantois↗
Electron microscopic observations concerning the action of some aromatic compounds on the morphology of Sendai virus.
The interaction between Sendai virus and some fluorescent aromatic compounds with reactive chemical groups ( a vinyl-sulfonic and an isocyano naphthalic acid derivative) leads to alterations of the surface and inner structure of virus corpuscles. The intensity of the changes depends on the type of reactive group. No such morphological changes were induced by 1-amino-2-anthraquinone-sulfonic acid, a compound whose interaction with the virus is exclusively of physical nature.
Anthraquinones↗
Investigations concerning the cultivation of myxo- and paramyxoviruses on chorioallantoic membrane fragments. Note I. Data on the multiplication of several myxo- and paramyxoviruses.
Influenza viruses A(H1N1) and A(H3N2) and parainfluenza viruses (Sendai mumps) were cultivated in chorioallantoic membrane (CAM) fragments maintained in media with different formulae, with or without daily medium changes, in roller or stationary tubes. Inoculation was performed either directly on CAM fragments in Petri dishes or by dilution of the virus-containing material in the medium. Infectant titers obtained in CAM fragments were similar to those recorded in embryonated eggs at 48 hours post inoculation (p.i.) in the case of influenza virus A(H1N1) and at 72 hours p.i. in that of Sendai and influenza A(H3N2) viruses; at 96 hours p.i. all the three viruses had titers superior to those found in the egg.
Animals↗
Comparative results of respiratory virus isolation attempts in chorioallantoic membrane fragments and embryonated chicken eggs.
Attempts performed with 39 samples of nasopharyngeal secretion resulted in the isolation of 21 (54%) hemagglutinating viral agents in chorioallantoic membrane (CAM) fragments, while a single strain (2.5%) was isolated in the embryonated egg. The time interval required for virus isolation in CAM fragments ranged from 4 to 20-24 days and was longer then 10 days in the embryonated egg. Maintenance of CAM fragments in roller tubes leads to a more abundant virus multiplication and, implicitly, to a reduction of the time interval necessary for virus isolation.
Allantois↗
Some characteristics of Sendai virus envelope solubilization by different detergents.
Gel-filtration through Sepharose-2B of Sendai virus envelopes solubilized by Tween-20 or Triton X-100 treatment allows the separation of two elution peaks, hemagglutinin (HA) activity being detected only in the peak corresponding to large envelope fragments. Solubilization of the same virus envelopes with N-octylglycoside (NOG) results in a single elution peak, exhibiting both HA and neuraminidase activities. The results obtained suggest : 1) the similar envelope composition of the different virus populations, and 2) the different mechanisms of solubilization, in relation to the detergent used for disruption. The advantages of virus disruption by NOG are discussed.
Chromatography, Gel↗
Relationships between ceruloplasmin and viral immunity.
Experimental data are reviewed with reference to the role of ceruloplasmin as a nonspecific factor of viral immunity. Ceruloplasmin would act a) by an inhibition of virus multiplication, resulting in a "slow-motion" infection, which promotes immunization; b) by leading to the synthesis of a virus population rich in defective interfering particles, and c) by the direct modulation of the immune response.
Animals↗
Experimental infection of soybean and transmission of Sendai virus through the seeds.
Sendai virus can multiply in the soybean and it can be serially transmitted over at least three passages. The presence of the virus was made evident in leaf homogenates from infected plants after several blind passages in egg chorioallantoic membrane fragments. The virus can be transmitted through the seeds of experimentally infected soybean plants and reisolated from leaves and roots.
Animals↗
Polycation-induced alterations of the envelope surface of influenza and parainfluenza viruses.
Natural macromolecular polycations (protamine, histones H1, H2A, H2B and H3) caused the clustering of influenza and parainfluenza viruses into aggregates heterogeneous in size, as well as a decrease in their hemagglutinating capacity. Polycation-induced virus alterations consisted in surface pyknosis and an increase most efficient as regards the interaction with viruses.
Chemical Phenomena↗
Comparative study of some characteristics of influenza virus A/PR8/34 (H1N1) cultivated on chorioallantoic membrane fragments in the presence of ceruloplasmin or of parainfluenza type I (Sendai) virus.
Multiplication of influenza virus A/PR8/34 (H1N1) in the presence of either ceruloplasmin or parainfluenza type I (Sendai) virus results in the appearance of progens different from the parental virus as regards some of their biological properties. The role of viral envelope changes in acquiring new characteristics is discussed.
Allantois↗
Investigations on the presence of viral and chlamydial antigens in cells exfoliated in the vagina of women with common and malignant gynecopathies.
A high proportion of parainfluenza, herpes, adenovirus and chlamydial antigens (10.7-36%) was detected by indirect immunofluorescence in cells exfoliated in the vagina of women with genital neoplasia and with uterine cervix ectopia and dysplasia. Much lower proportions of the same antigens were found in patients with common gynecopathies or recurrent genital herpes and in pregnant women. The possible relationships between chronic virus infections and genital neoplasia in women are discussed.
Antigens, Bacterial↗