Biomedical subjects

I Savi

Publications and source records attributed to I Savi.

8 recordsLinked to original sources

PubMed1978

[Study of blood viscosity and fibrinogen as a guide to the therapy of atherosclerotic peripheral arteriopathy].

Of the many blood parameters now regarded as useful in the study of arteriopathy particular importance is attached to viscosity and fibrinogen levels. Changes in viscosity lead to changes in flow and the supply of O2 to the tissues, while fibrinogen alterations frequently accompany or precede atherosclerosis, since they both encourage the intravascular deposition of fibrin and help to modify viscosity. The pharmacological correction of such changes is discussed.

Adult↗

PubMedDate not supplied

Correlation of serum interferon with some clinical and humoral signs of systemic lupus erythematosus.

The serum level of total interferon (IFN) was measured in 15 male patients with systemic lupus erythematosus (SLE) in the active phase and in remission, before and during corticotherapy. The values found were correlated with the clinical and humoral signs of disease. The IFN titer was high in the active phase of disease and was correlated with fever, extension of skin rash, polyarthritis, myositis, autoimmune hemolysis, cardiac and cerebral involvement as well as with ESR, reacting protein C, CIC, ANF and the percentage of LE cells. Isolated LE nephropathy without rapidly progressive or advanced renal failure was not associated with high IFN titer.

Adult↗

PubMedDate not supplied

The effect of human alpha leukocyte interferon (IFN) on the frequency of sister chromatid exchanges (SCE) in the KB cell line.

Concentrations of 50, 500 and 5,000 iu/ml of natural human alpha leukocyte interferon (IFN) were added into the culture medium of KB cells, 4 h after serial passage, in the presence of 5-bromo-desoxyuridine (Brdu) at 10 micrograms/ml. Similar cultures without IFN were set up as controls. After 72 h of incubation, the harlequin technique (differential staining of sister chromatids) was applied in order to discriminate among the metaphases of different generations and to appreciate the frequency of sister chromatid exchanges (SCE) in the second generation cells. The incidence of sister chromatid exchanges was slightly increased following IFN treatment but no dose-effect relationships were observed. At the same time, cell cycle kinetics estimated as replication index (RI) and average generation time (AGT) was not modified in IFN-treated cells as against the controls.

Cell Division↗

PubMedDate not supplied

S-phase detailed analysis in KB cells by bromdeoxyuridine labelling.

A method based on the sequential BrdU incorporation followed by differential spiralization of the chromosomes was used for analysis of S-phase in KB cells when Brdu was added to the cultures for 19, 24 and 26 hours before fixation. The S-phase was subdivided into three subphases, each recognisable by a specific incorporation pattern at metaphase: late-S (LS), middle-S (MS) and early -S (ES). Two other intermediary subphases, mid-early S (mES) and mid-late S (mLS) have also been distinguished but their accurate identification is generally difficult. A high degree of asynchrony in KB cells population was observed as well as a wide variability concerning the duration of S-phase (BrdU = bromdeoxyuridine).

Bromodeoxyuridine↗

PubMedDate not supplied

Diagnostic value of serum interferon level in systemic lupus erythematosus.

In all the patients with systemic lupus erythematosus (SLE) tested in the active phase of the disease the serum interferon (IFN) level increases above the normal value (20 I.U./ml) reaching in some cases 80-160 I.U./ml. The detection of this increased level of IFN is useful to discriminate if some diseases such as glomerulonephritis or hemolytic anemias develop as part of a systemic auto-immune disorder since in such pathologic conditions lacking auto-immune mechanisms the serum IFN level is not increased. The detection of the serum IFN level proved to be a useful test in the follow-up of the clinical evolution as well as for the efficiency of the immunosuppressive therapy since in all the investigated cases IFN level decreased to normal after Cortisone administration concomitantly with the clinical remission of the disease. The above described data support the assumption that in the pathogeny of auto-immune diseases, SLE in our case, there are some initial cell-mediated auto-immune phenomena such as cell-mediated auto-cytotoxicity leading to a massive activation of the CTL and NK cells and consequently to the over-production of some lymphokines such as IFN.

Autoimmune Diseases↗

PubMedDate not supplied

Interferon level in human normal sera.

Interferon (IFN) level in the sera of normal humans was determined by their protective effect on a KB cell culture against the cytopathic effect (CPE) induced by vesicular stomatitis virus. The technique revealed all the three known types of IFN. In the investigated population 15 of 37 sera (40%) were found to protect against the CPE at 1/10 dilution, 11 of 37 (30%) still protect at 1/20 dilution, and 7 of 37 sera (19%) still protect at a dilution of 1/40. No serum was found to have detectable IFN levels at 1/80 dilution. We can thus consider that the normal serum level ranges up to a 1/20 dilution i.e., up to 20 IU/ml. The above titers are slightly higher than the normal IFN level reported by other investigators in other human populations, although no clear reasons can be given to account for the differences.

Adult↗

PubMedDate not supplied

Ginterferon (human leukocyte interferon) efficiency in the treatment of viral diseases. 1. Efficiency in viral keratoconjunctivitis.

Ginterferon is a human leukocyte interferon (IFN) produced in the "V. Babes" Institute by in vitro culture of leukocytes separated from neonatal cord blood. Ginterferon (50,000 IU/ml) was administered to 40 patients who suffered for at least two weeks of a hepatic keratoconjunctivitis resistant to treatment with conventional regimens of human immunoglobulins, Iduviran or topical antiinflammatory drugs. In 35 cases (87%) complete recovery (total epithelialization and disappearance of all symptoms) occurred in a significantly shorter time (10-14 days) as compared to similar cases which recovered after 30-40 days by conventional treatment. Local or general untoward effects were not observed in any of the patients. Recovery in the remaining 5 cases was slower, 28-30 days. This slow response may have been due to unusual weakness of the general immunobiological condition in these patients (myocardial infarction or repeated stress) which might have favoured repeated waves of virus spreads from foci of viral latency which are not attained by the topically administered IFN.

Drug Evaluation↗

PubMedDate not supplied

The use of differential staining of sister chromatid to estimate the in vitro effect of human alpha interferon on cell division in normal and tumour cells.

Concentration of 10, 100 and 1000 I.U./ml of human leukocyte alpha interferon (IFN) were added into peripheral human blood (PBL) cultures and in KB cell cultures in the presence of 5-bromdeoxyuridine (BrdU) at 10 micrograms/ml. After 72 hours the differential staining of sister chromatid (harlequin) technique was applied in order to differentiate among the metaphases of successive cell generations occurring in the presence of IFN. The frequency of the first (M1), second (M2) and third (M3) metaphases was recorded and the replication index (RI) as well as the average generation time (AGT) was calculated for untreated controls and for each of the IFN concentrations used, both in the blood cultures and in the KB cells. In the PBL cultures a clear dose-related inhibitory effect of IFN on cell division was observed, the RI values being lessened whereas the AGT concomitantly increased by increasing the IFN concentrations. An increase in M1 metaphase frequency was observed concomitantly with a diminished number of M3 cells. In KB cells the division kinetics was not influenced by IFN as indicated by similar RI and AGT values observed in controls and in IFN treated cells. However, the frequencies of both M1 and M3 cells were slightly diminished concomitantly with a discrete augmentation of M2 cell number. The differential staining of sister chromatid thus proved a highly useful technique to investigate the different sensitivity of the normal and malignant cells to the growth inhibitory effect induced by alpha IFN in vitro.

Bromodeoxyuridine↗