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Biomedical subjects

I Sechter

Publications and source records attributed to I Sechter.

At least 37 records · Page 2Linked to original sources
PubMed1983

Extended scheme for serotyping Campylobacter jejuni: results obtained in Israel from 1980 to 1981.

The serotyping scheme for Campylobacter jejuni previously developed in the National Center for Campylobacter, Jerusalem, was extended by the use of 20 new sera and modified by the absorption of the sera, when necessary, with homologous boiled cultures or heterologous live cultures. The extended scheme is based on slide agglutination of live suspensions and is performed in two stages: pretesting with four pooled sera and final testing with monovalent sera. So far, 34 serotypes have been recognized. Among 442 isolates of C. jejuni, 86.4% could be typed with this scheme. Of the 382 cultures typed, 90% reacted with one single serum, and the remainder showed a complex antigenic structure. The frequent serotypes identified were: 11 (12.9%), 12 (8.2%), 18 (6.3%), and 3 (6.1%). When epidemiological data were available, the results of the serotyping corresponded with the epidemiological evidence.

Antibodies, Bacterial↗
PubMed1983

Survey of pneumococcal types in Israel, 1978-81.

The most common capsular types of Streptococcus pneumoniae that prevailed among 436 cultures isolated in Israel (1978 through 1981) were: 1, 14, 6B, 23F, 19A and 5. Among the 301 isolates from significant sources, 66.1% belonged to 14 capsular types that are included in available pneumococcal vaccines. Among those that were not included in these vaccines, the most frequent types were: 5, 19A and 6B. The addition of these three capsular polysaccharide types to the vaccine used in Israel would result in a vaccine coverage of approximately 85%.

Adolescent↗
PubMed1981

Improved method for cholera diagnosis.

The use of the oxidase test is proposed to discover sparse colonies of cholera vibrios on a plate of Pril nutrient agar seeded directly with the suspected stool sample or after enrichment. This method also enables the detection of other oxidase-positive potential pathogens, such as non-agglutinating vibrios, Aeromonas hydrophila, Pseudomonas spp., and other nonfermentative organisms.

Adolescent↗
PubMed1981

Antigenic scheme of Citrobacter koseri.

An antigenic scheme, based on the determination of 17 somatic and 9 flagellar antigens, is proposed for Citrobacter koseri. Seven of the flagellar antigens constituted the specific phase (a through g), and the others (1 and 2) were nonspecific. Of the 238 theoretically possible serotypes, 48 were identified among 680 cultures examined.

Animals↗
PubMed1981

[Sero- and biovars of Levinea malonatica (syn. Citrobacter diversus, Citrobacter koseri) in clinical material (author's transl)].

The biochemical and serological examination of 31 strains of Levinea malonatica isolated from faeces, urine, sputum, wound infections and blood showed no correlation of bio- or serovars with the origin of the strains. Serological cross-reactions between O-antigens of L. malonatica and certain Salmonella, Shigella and Yersinia enterocolitica serovars were analysed. They are low-titred and seem to be of minor importance. Sensitivity testing revealed the resistance of L. malonatica against penicillin, ampicillin and carbenicillin.

Anti-Bacterial Agents↗
PubMed1977

Six new Salmonella sub-genus III serotypes isolated from snakes in Israel.

Six new Salmonella sub-genus III serotypes (Arizona) were isolated from snakes in Israel. Their antigenic structure was established as follows: S. arizonae 16:Z52:Z35 (Ar.25:26-21); S. arizonae 18:1,v:e,n,x,z15 (Ar.7a,7b:23-28); S. arizonae 21:i:1,5(7) (Ar.22:33-30); S. arizonae 41:c:e,n,x,z15 (Ar.13:32-28); S. arizonae 51:k:z35 (Ar.1,2:29-21); and S. arizonae 53:1,v:z35 (Ar.1,4:23-21).

Animals↗
PubMed1976

Citrobacter diversus isolated from clinical material.

Forty-seven strains of Citrobacter diversus were isolated during a 12-month period from clinical material obtained from patients in a general hospital in Israel. The majority of cultures (38) were recovered from urine and wound discharges. There was one case of septicemia. The biochemical reactions of all cultures were typical for this species, except one that was anaerogenic, and they could be divided into five biotypes (a through e). Thirty-nine strains were identified serologically and found to belong to one of seven O groups described by Gross and Rowe (1974). All cultures were sensitive to tetracycline and nalidixic acid. All strains were resistant to carbenicillin and ampicillin and produced beta-lactamase.

Ampicillin↗
PubMed1976

Salmonella and Arizona from snakes in the Judean desert (1974-1975).

Ninety-four (77.7%) of the 121 snakes captured during the years 1974-1975 in the Judean desert were found to carry Salmonella and/or Arizona organism. Sixty-six snakes (54.4%) harboured only one serotype. From 19 animals (15.7%) both Salmonella and Arizona organisms were isolated. From 9 snakes (7.5%) 2 or 3 serotypes of Arizona were isolated. Only 27 snakes (22,3%) harboured no detectable Salmonella nor Arizona in their intestinal contents.

Animals↗
PubMed1975

Method for detecting small numbers of Vibrio cholerae in very polluted substrates.

A method is presented for the indirect detection of Vibrio cholerae by the multiplication of two specific bacteriophages: phiH74/64 for El-Tor vibrios, and phage group IV (Mukerjee) for classical vibrios. The product to be examined is seeded in alkaline tryptone water for enrichment, as in the classical method, and is then incubated for 6 h at 37 C. Thereafter, a loopful is transferred to each of two nutrient broth (pH 9) tubes. One of these receives a drop of phage phiH74/64; the other receives a drop of phage group IV. The stock phages are diluted so as to contain about 3,800 plaque-forming units in one drop; this is the maximum amount which, when added to 10 ml of broth, will not be detected in a loopful of 1 mm diameter. The tubes containing phage phiH74/64 are incubated at 42 C; those with phage group IV are incubated at 37 C. After 18 h the cultures are killed by agitation with chloroform, and a 1-mm loopful is deposited on a layer seeded with the detector strains: Makassar 757 for El-Tor phage and V. cholerae 154 for classical cholera phage. After 4 to 5 h at 37 C, lysis appears on the spot areas if there has been phage multiplication in the respective broth tubes. With experimentally contaminated sewage water, vegetables, or stools, 1 to 10 cholera vibrios were detected in every sample. In rare cases, false-positive results were obtained by multiplication of the phage on non-cholera vibrios.

Bacteriological Techniques↗
PubMed1975

Viability of Vibrio cholerae biotype El Tor and of cholera phage on vegetables.

Different vegetables were contaminated with sewage water containing large amounts of El Tor vibrios, and the viability of these vibrios was studied under defined conditions of storage. During the dry season (summer) in Jerusalem, the El Tor vibrios survived for up to 24 hr on parsley, 24 to 30 hr on tomatoes and carrots, 24 to 48 hr on cucumbers, peppers and okra and two to three days on lettuce, when the vegetables were stored at room temperature. The survival time was longer during the rainy season or during storage of the vegetables in a refrigerator. It was also prolonged on damaged vegetables. The El Tor vibrios survived for only a few hours on vegetables exposed to sunlight. The El Tor vibrios survived for 12 to 24 days in experimentally contaminated sewage water, and for up to 10 days in sewage-contaminated soil. The El Tor bacteriophage persisted for up to 36 days on phage-contaminated vegetables.

Bacteriophages↗