I Suvaizdis | PubMed Health

PubMed1994

Injuries induced by diffuse photodynamic action in retina and choroid of albino rats. Morphologic study of an experimental model.

BACKGROUND: The morphologic changes of the retina, choroid, and respective vasculatures in an experimental model of diffuse photothrombosis and photodynamic injury were studied. METHODS: After intravenous injection of rose bengal (40 mg/kg), eyes of 21 albino rats were exposed to a light intensity of 15-17,000 ft-cd for 2 to 15 minutes. Survival times ranged from 15 minutes to 6 days. The specimens were studied by light and electron microscopy. RESULTS: Photothrombosis of retinal and choroidal vessels was variable in extension and duration. Vascular lesions included vacuolation and sloughing off of endothelium, and necrosis and apoptosis of pericytes and smooth muscle cells. Retinal and choroidal injuries exhibited considerable regional and structural differences, such as predominance in the outer versus inner retina, or focal versus diffuse injury. CONCLUSION: Damage produced by this model was irregular in extent and character, due to several hard-to-control parameters. It was presumably due not only to ischemia but also to free radical chain reactions initiated by photodynamic action. This model may not be suitable for rapid quantitative assessment of ischemic retinal injury, but it provides opportunities for the investigation of pathophysiologic mechanisms.

Animals↗

PubMed1984

Inherited snowflake cataracts.

The authors report two unusual pedigrees of flake-like cataracts inherited as an isolated mendelian trait, presumably autosomal dominant. The most striking biomicroscopic feature is an accumulation of large white irregular flakes ('type B opacities') scattered throughout the equatorial cortex, with apparently less involvement of the nucleus. Some of these opacities are globular, but most are flattened and have feathery edges. These type B opacities are clinically and microscopically identical in the two families. Type B lesions are characterized histologically by irregular pale-staining areas. Ultrastructurally, these areas show large collections of microglobular degeneration immediately adjacent to normal-appearing lens fibers. Electron probe studies demonstrate normal calcium levels. 'Type A' opacities are biomicroscopically smaller, punctate, and clinically non-specific. Ultrastructurally, type A opacities include clusters of medium sized lenticular globules, each of which is larger than the individual microglobular abnormalities of type B lesions, although the aggregated type B lesion is considerably larger than an individual type A lesion. The microscopic appearance of this inherited cataract appears to be unique.

Adult↗