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Biomedical subjects

I Svoboda-Beusan

Publications and source records attributed to I Svoboda-Beusan.

9 recordsLinked to original sources
PubMed2006

PMA and doxorubicin decrease viability, MTT activity and expression of CD10 marker on NALM-1 leukemic cells.

PMA (10, 20 ng/ml) and doxorubicin (5-20 ng/ml) decreased the viability and MTT-activity of NALM-1 pre-B leukemic cells (3 days' treatment). Further, CD10 was downregulated, suggesting that PMA and doxorubicin induced differentiation of NALM-1 cells. However, PMA did not alter expression of B cell markers CD20 and of mIgM. In contrast to PMA, another differentiation agent ATRA did not alter CD10 expression on NALM-1 cells but affected viability after 6 days (5, 10 ng/ml). The data in this study are the first evidence that PMA and doxorubicin inhibited viability and MTT activity and induced partial differentiation, by decreasing CD10 on NALM-1 cells.

Antibiotics, Antineoplastic↗
PubMed2000

The relevance of multidrug resistance-associated P-glycoprotein expression in the treatment response of B-cell chronic lymphocytic leukemia.

BACKGROUND AND OBJECTIVES: The aim of this study was to determine whether expression of P-glycoprotein (Pgp) is an intrinsic feature of B-lymphocytes in B-cell chronic lymphocytic leukemia (B-CLL) and how it correlates with hematologic indices and tumor load in the disease. Furthermore, the change of Pgp expression under cytotoxic treatment and its correlation to treatment outcome were studied. DESIGN AND METHODS: In 42 B-CLL patients, of whom 13 were sequentially monitored, expression of extracellular (MRK-16) and intracellular (C-219) Pgp epitopes on peripheral blood lymphocytes was determined by flow cytometry analysis and quantified by ratio of the mean fluorescence (RMF) in flow cytometry analysis. RESULTS: Median RMF values in B-CLL patients were higher than in age-matched controls. Pgp expression did not correlate with any of the hematologic features or clinical stage of the disease. Patients who received some type of cytoreductive treatment prior to the study had lower Pgp values for both measured epitopes (median RMF for C-219 and MRK-16 of 1.99 and 2.03 in comparison to those of non-treated patients: 3.11 and 2.88, respectively). In 13 patients monitored during treatment the decrease in RMF was noted after treatment with chlorambucil, with RMF values for both Pgp epitopes decreasing in responders. This was in contrast to unchanged or even increased RMF values in those patients who did not respond to therapy. INTERPRETATION AND CONCLUSIONS: Our study confirms the importance of quantitative evaluation of Pgp expression by flow cytometry. At the clinical level, cross-sectional, single test evaluation of Pgp is of limited value whereas sequential follow-up values correlate with treatment response.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed1994

Flow-cytometric lymphocyte phenotyping using automated SimulSET Software gating procedure.

The peripheral blood lymphocyte phenotypes of 20 healthy young normal subjects between the ages 21 and 29 were determined by dual fluorescence analysis with fluorochrome--labeled monoclonal antibodies (mAbs). Samples were prepared by a whole blood lysis technique and analysed on a FACScan flow cytometer using SimulSET Software. Values for lymphocyte subpopulations (percentage and absolute count) are presented. Some values are different in relation to sex. The most important steps in SimulSET software gating procedure of lymphocytes are also described.

Adult↗
PubMed1993

Multiple changes of immunologic parameters in prisoners of war. Assessments after release from a camp in Manjaca, Bosnia.

OBJECTIVE: To assess immune reactivity in men just released from a war prisoner camp. PARTICIPANTS: Random sample of 29 men from a group of 764 liberated detainees in war prisoner camp in Bosnia, 15 matched healthy control subjects, and pre-war historical control subjects. MAIN OUTCOME MEASURES: Report on immune reactivity parameters, such as lymphocyte immunophenotypes, natural killer cell and phagocyte function, serum cytokines, and hormones. RESULTS: Compared with control subjects, detainees had significantly lowered red blood cell count, hemoglobin mass concentration, hematocrit, total serum proteins, and albumin level, while the percentage and count of monocytes and non-segmented neutrophils were increased. Flow cytometry revealed a significant increase in percentage of activated lymphocytes, activated T lymphocytes, Tc/s lymphocytes, B lymphocytes, and total HLA-DR lymphocytes. The absolute counts of activated lymphocytes and activated T lymphocytes were also significantly increased. The percentages of naive Th/i lymphocytes and the ratio of CD4:CD8 lymphocytes were decreased. The in vitro natural killer cell cytotoxic activity and phagocytic functions of ingestion and digestion were significantly depressed. Serum interferon, serum cortisol, and prolactin were also significantly lowered. Serum tumor necrosis factor was increased. CONCLUSIONS: Alterations in the main parameters of the immune system and depression of important immune effector functions may have resulted from the psychological stress, physical deprivation, and malnutrition experienced by these war camp prisoners during their detainment.

Adolescent↗
PubMed1993

T-cell subsets in asthmatic children.

Using monoclonal antibodies authors determined the percentage of CD2(+)-, CD4(+)- and CD8(+)-lymphocytes in the peripheral blood of 45 asthmatic children. In 38 subjects asthma was reaginic, while in 7 it was nonreaginic. The aim of the study was to detect an abnormality which might contribute to the pathogenesis of the disease. A correlation test was done to assess the association of the above-mentioned percentages with the serum IgE level and the duration of the illness. The percentage of CD2(+)-lymphocytes and CD4+ lymphocytes does not differ significantly among the patient groups themselves nor in relation to healthy children. The percentage of CD8(+)-lymphocytes in asthmatic children, particularly in the group with reaginic asthma, is significantly lower (P < 0.01) than in healthy children. A significant negative correlation (P < 0.05) was found between the percentage of CD8(+)-cells and the serum IgE level in asthmatics. There was no significant correlation between the CD2(+)-lymphocyte and CD(4+)-lymphocyte percentages and the serum IgE level, nor between any of examined percentages and the duration of the illness. The authors conclude that, in children with reaginic asthma, a primarily lower percentage of CD8(+)-lymphocytes, which bear a suppressor function, may be one of the causes for over-production of IgE and is therefore an important factor in the pathogenesis of the illness.

Adolescent↗
PubMed1991

Flow cytometric analysis of lymphocytic subsets in haemorrhagic fever with renal syndrome virus infection.

Percentage counts of T-lymphocytes (helper and suppressor subpopulation) and B-lymphocytes were performed in the peripheral blood of two confirmed cases of HFRS infection using Coulter Clone monoclonal antibodies and Becton Dickinson flow cytometry. The individual values were compared with those from healthy subjects. HFRS patients had lower total T-cell (CD2) and T-helper (CD4 subset) counts. This seems linked with the role of the CD4 determinant as viral receptor. The measuring of specific lymphocyte helper and suppressor subpopulations might be useful in predicting the course of the disease and in following the effects of therapy.

B-Lymphocytes↗
PubMed1991

[Reference values in immunodiagnosis: the approach using the Senieur protocol].

In an attempt to standardize the normal values for immunological tests it is necessary to establish strict admission criteria. The EURAGE Concerted Action Programme on Ageing of the European Community described the SENIEUR protocol with criteria for immunological studies in man, based on clinical and laboratory information. Our results on humoral and cellular components of immunity in dependence of age, according to SENIEUR protocol admission criteria are presented.

Adolescent↗
PubMedDate not supplied

[Multiple drug resistance].

The multidrug-resistance (MDR) phenotype expressed in mammalian cell lines is complex. A cell selected with a single agent can acquire cross-resistance to a remarkably wide range of compounds which have no structural or functional similarities. The basis for cross-resistance seems to be a decreased net cellular accumulation of the drugs involved, and has been attributed to alterations in plasma membrane. An over-expressed plasma membrane P glycoprotein of relative molecular mass of 170 kD (Pgp) is consistently found in different MDR human and animal cell lines, and in transplantable tumors. Consequently, it has been postulated that Pgp directly or indirectly mediates MDR. This paper reviews the current knowledge on etiology, pathogenesis, diagnosis and therapy of MDR.

Animals↗