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Biomedical subjects

I Takeuchi

Publications and source records attributed to I Takeuchi.

At least 127 records · Page 7Linked to original sources
PubMed1981

Bivariate ratio monitoring in clinical laboratories.

The usefulness of monitoring bivariates was studied and discussed. Analysis of the correlation between bivariates was studied and discussed. Analysis of the correlation between bivariates demonstrated that combinations of bivariates can be divided into two groups. The first group was the combination in which a close correlation between two variates could be observed in any pathophysiologic state. The monitoring of the ratio of this first group was effective for the detection of analytical mistakes and the detection of symptom poor hereditary disorders, such as pharmacogenetical disorders. The second group was one in which a high correlation between two variates was obtained in some specific pathologic stage, and the monitoring the ratio of this second group gave high predictive values for the screening of that pathologic stage. It was demonstrated that the use of bivariate monitoring was not only very simple but also provided an effective laboratory data analysis system for the laboratory routine.

Aspartate Aminotransferases↗
PubMed1981

Role of cell sorting in pattern formation in Dictyostelium discoideum.

To examine the relationship between cell sorting and cell differentiation in the development of Dictyostelium discoideum, labeled cells grown in the absence of glucose [G(-)cells] and unlabeled cells grown in its presence [G(+)cells] were mixed and either allowed to undergo normal morphogenesis or cultured under submerged conditions. Changes in the distributions within a cell aggregate of labeled cells and cells stained with the conjugated antispore serum (prespore cells) were followed on the same sections by the methods of autoradiography and immunohistochemistry. In normal morphogenesis, differentiation of prespore cells apparently initiated and proceeded coincident with sorting out between G(+) and G(-) cells, during formation of standing slug. By contrast, within an aggregate formed under submerged conditions, prespore cells began to differentiate long before G(+) and G(-) cells were sorted out, indicating that the cell sorting is not a prerequisite for the cell differentiation. The sorting out, however, brought about an accumulation of prespore cells in a hemisphere, thus producing a prestalk-prespore pattern within the aggregate.

Cell Aggregation↗
PubMed1980

Hereditary deficiency of lactate dehydrogenase M-subunit.

A family with a complete deficiency of lactate dehydrogenase M-subunit was investigated. The propositus was an 18-year-old male who complained of exertional pigmenturia and easy fatigue. Marked discrepancy was observed in the ratio between creatine kinase and lactate dehydrogenase (CK/LDH). Electrophoretic analysis of serum LDH isoenzymes of the propositus demonstrated only one activity band of LDH H4. A complete lack of the LDH M-subunit was similarly demonstrated in erythrocytes, leukocytes and in the intermediate vastus muscle. LDH levels in the muscle specimen were markedly decreased in the patient, whereas CK and aspartate aminotransferase were almost the same as in a control subject. LDH isoenzymes of erythrocytes were analyzed in 5 siblings and in the parents. This demonstrated a complete lack of LDH M-subunit in 3 siblings. The ratio between H-subunit and M-subunit (H/M) in erythrocyte LDH suggested a partial absence of the M-subunit in two siblings and in the parents. An abortive increase of blood lactate and a marked increase in blood pyruvate were observed immediately after ischemic work of the forearm, accompanied by an increase in serum creatine kinase and myoglobinuria. The present case represents a newly described form of genetically determined myopathy.

Adolescent↗
PubMed1980

Very low density lipoprotein binding to adipocytes.

Human very low density lipoprotein (VLDL) has been found to interact with both isolated adipocytes and adipocyte membranes in a manner which is saturable, reversible and dependent on time and temperature. Except for a difference in maximum binding capacity, a similar pattern is seen with both rat epididymal adipocytes and human omental adipocytes. The capacity of rat cells is 0.04 micrograms VLDL per 2 x 10(5) cells. For human cells the capacity is 0.321 micrograms VLDL per 2 x 10(5) cells. Scatchard plots of the competition data are linear. This, and dissociation studies conducted in the presence or absence of unlabelled VLDL suggest that there is no cooperative interaction between the binding sites. Unlabelled VLDL and HDL each compete equally with 125I-labelled VLDL for binding sites. LDL is 25 times weaker as a competitor. Intralipid and unrelated peptides have no effect. These data suggest that the ligand is not apolipoprotein B and not apolipoprotein A. The competitive effect of HDL is not dependent on its apolipoprotein E content. A preparation of C apolipoproteins (75% C-11) is as potent as unlabelled VLDL in competing with 125I-labelled VLDL for binding sites. These data indicate that VLDL can bind to adipocytes. The receptor can interact with other lipoproteins. If differs from the LDL receptor as it does not interact with apolipoprotein B or apolipoprotein E, but binds to a C apolipoprotein.

Adipose Tissue↗
PubMed1980

Motive force of the migrating pseudoplasmodium of the cellular slime mould Dictyostelium discoideum.

Motive forces of migrating pseudoplasmodia (slugs) of Dictyostelium discoideum were determined by application of a double-chamber method. The motive force of a whole slug was proportional to its volume, the value per unit volume being 5.85 x 10(-6) dyne/cm3 (58.5 N cm-3). The motive force was independent of temperature (13.5--26 degrees C) and decreased during prolonged migration. Motive force per unit volume of an anterior isolate of a slug was much larger than that of a posterior isolate, their weighted mean being approximately equal to that of a whole slug. These results agree well with the predictions previously made using a model based on analyses of migrating velocities of slugs. The motive force per unit volume of either isolate was soon regulated to reach the normal value of an intact slug after several hours of isolation, concurrently with conversion of cell types between prestalk and prespore cells. The possibility that motive force of each cell is determined by its cell type is discussed in relation to cell sorting.

Dictyostelium↗
PubMed1979

Repression of rRNA synthesis induced by disaggregation in Dictyostelium discoideum.

Cells disaggregated from the slugs of Dictyostelium discoideum continued to incorporate [3H]uridine or [3H]uracil, though at a lower rate than interphase amoebae. Disaggregation brought about a marked increase in the proportion of labeled poly(A)-containing RNA to labeled total RNA. The proportion reached the maximum in disaggregated cells after 1 h of incubation, but remained high even after 5 h of incubation. Reconstruction of slugs from the disaggregated cells did not bring about a decrease but a further increase in the proportion. The real proportion estimated from the minimum period of labeling reached almost 100% in the disaggregated cells, in contrast to about 30% in the interphase amoebae. The increase in the proportion is attributed to the disaggregation-induced repression of rRNA synthesis, which was supported by sucrose density gradient analyses of RNA synthesized in the disaggregated cells. The possibility that rRNA synthesis in this organism is regulated by the loss of cell contact is discussed.

Cell Division↗
PubMed1979

Sorting out behaviour of disaggregated cells in the absence of morphogenesis in Dictyostelium discoideum.

Cells disaggregated from slugs of Dictyostelium discoideum were cultured in Bonner's salt solution in roller tubes. Cells rapidly stuck together to form an amorphous loose agglutinate which was later transformed into a spheroidal tight agglutinate surrounded by slime sheath material. Prespore cells in the loose agglutinate underwent partial dedifferentiation by starting to decompose their specific antigen until formation of the tight agglutinate, in which the antigen was resynthesized. During the process, there was some decrease in the proportion of prespore cells. Changes in the distribution of prespore and prestalk cells in the agglutinates were examined by using immunocytochemical staining. They were randomly distributed in the early agglutinates, but became well separated in 4 h agglutinates in such a way that prestalk cells were completely enveloped by prespore cells. Prestalk cells later came outside to be partially enveloped and finally occupied a hemisphere side by side with prespore cells. During the process, cells in one or two outer layers differentiated into prestalk or stalk cells. Similar changes in the distribution pattern were observed, when labelled prestalk cells were cultured with unlabelled prespore cells and their distribution in co-agglutinates was followed by autoradiography. It was concluded from these results that the majorities of prestalk and prespore cells isolated from slugs are sorted out in agglutinates without changing their original cell types, and that the sorting-out occurs in the complete absence of polar structures and movement of the cell mass. The distribution patterns in agglutinates of prestalk and prespore cells were discussed with reference to intercellular adhesion among/between them.

Agglutination↗