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Biomedical subjects

I Testa

Publications and source records attributed to I Testa.

At least 55 records · Page 3Linked to original sources
PubMed1987

Human growth hormone induced modulation of hepatitis B virus (HBV) gene expression.

A well characterized human hepatocellular carcinoma cell line (PLC/PRF/5) containing complete sequences of HBV-DNA in integrated form into host DNA and growing under serum-free conditions was used to test the effect of human growth hormone (hGH) on the expression of the integrated viral DNA. Data in our hands point to an early, specific effect of hGH on HBV-DNA integrated sequences which appears to be unrelated to the late effect on total protein synthesis. Moreover this approach enabled us to detect specific hGH receptors on the cell membrane of PLC/PRF/5 cell line.

Animals↗
PubMed1985

[Rapid method for evaluating intra-erythrocyte Na+ activity].

The measurements of intracellular "Na+ activity" was performed in 10 ml of heparinized venous blood. First the blood was three times washed in isotonic magnesium chloride solution (114 mmol/l). Thereby the buffy coat was removed. Then the microhematrocrit was taken for packet cell volume determination. After the erythrocytes were lysed by ultrasound. Sodium "Na+ Activity" is measured in the hemolysate by Ion-Selective electrode. With this method all "pipetting" operations are eliminated and for the "Na+ activity" determination was used ion-selective electrode with an indirect measurements, which is less influenced by the matrix. Reference intervals determined for a healthy population were 7.3 +/- 0.6 mmol/l.

Adolescent↗
PubMed1985

[Evaluation of intra-erythrocyte Na+ activity in persons at high risk for essential arterial hypertension and as an aid in differential diagnosis from secondary forms of hypertension].

The intracellular "Na+ activity" was measured in erythrocytes of normotensive subjects (46), in essential hypertensive patients (18), in their children (20) and in patients with secondary hypertension (8). In normotensive subjects without a genetic trait of hypertension intracellular "Na+ activity" was 7.3 +/- 0.8 mmol/l, in secondary hypertensive patients was 7.5 +/- 0.6 mmol/l, in essential hypertensive patients was 10.9 +/- 1.1 mmol/l and in their children was 8.6 +/- 2.1 mmol/l. In this group (children) it was possible to differentiate between 2 population, the 1 degree with height intracellular "Na+ activity" (8); the 2 degrees with normal intracellular "Na+ activity".

Diagnosis, Differential↗
PubMed1985

Tissue-specific antibodies against the fibroblast insulin receptor in a patient with lupus nephritis and hypoglycemia.

We recently reported that the serum from a patient with lupus nephritis, insulin resistance, and hypoglycemia contains multiple populations of antibodies directed at the human insulin receptor. In the present study, we found a subpopulation of antibodies (eluted from a protein A-Sepharose affinity column at pH 4.3) directed at the human fibroblast insulin receptor. When tested against human placental membranes, IM-9 lymphocytes, circulating monocytes and erythrocytes, and isolated adipocytes, the antibody subpopulation did not compete with 125I-insulin for binding to its receptor. In contrast, the antibody subpopulation competed with 125I-insulin for binding to the human fibroblast insulin receptor. This antibody subpopulation stimulated [3H]alpha-aminoisobutyric acid [( 3H]AIB) uptake to these cells. Unlike the effect of insulin, however, this regulation of transport was not antagonized by a mouse monoclonal antibody to the human insulin receptor that inhibits 125I-insulin binding. These studies indicate, therefore, that a tissue-specific antibody subpopulation can occur spontaneously in patients with antibodies to the human insulin receptor. Furthermore, they indicate the presence of anti-insulin receptor autoantibodies specifically directed against a tissue that is not primarily involved in glucose metabolism.

Animals↗
PubMed1985

Effect of insulin on an insulin receptor of PLC/PRF/5 human hepatoma cell line.

A human hepatoma cell line (PLC/PRF/5) contains several copies of hepatitis B virus (HBV) DNA in integrated form and releases hepatitis B surface antigen (HBsAg) in the form of 22 nm particles in culture medium; studies of the supernatant fluid failed to provide evidence of the morphologically intact virion (Dane particle) or hepatitis B infectivity. In this paper we describe the effect of insulin on cell growth and HBsAg production by these cells; moreover we describe the insulin binding to specific cell membrane receptors. Data in our hands point to a different insulin binding related to different incubation conditions.

Binding, Competitive↗
PubMed1984

Insulin reduces HBsAg production by PLC/PRF/5 human hepatoma cell line. Brief report.

Although its action at the molecular level is not completely understood, insulin, as well as its antagonist glucagon, certainly plays an important role in the modulation of protein synthesis. In order to observe whether insulin is involved in virus gene expression, we studied its effect on PLC/PRF/5 human hepatoma cell line, which posses HBV DNA sequences integrated at several sites. While human insulin had no effect on cell growth and increased the production of two plasma proteins, a selective inhibitory effect on HBsAg production could be detected. This observation might be useful for further studies both on virus gene expression and insulin action at the molecular level.

Carcinoma, Hepatocellular↗
PubMed1982

Decreased insulin receptors but normal glucose metabolism in Duchenne muscular dystrophy.

Compared to matched controls, 17 patients with Duchenne muscular dystrophy showed decreased insulin binding to monocytes due to decreased receptor concentration. These patients showed no signs of altered glucose metabolism and retrospective analysis of the clinical records of a further 56 such patients revealed no modification in carbohydrate metabolism. These data suggest that reduced insulin receptor number does not produce overt modifications of glucose metabolism in Duchenne muscular dystrophy.

Adolescent↗
PubMed1980

[Glycemia and insulinemia after oral administration of Nomifensine].

Blood glucose and immunoreactive insulin (IRI) were evaluated in 8 healthy subjects after mg 200 of Nomifensina per os. 5 subjects underwent an oral glucose challenge before and after mg 200 of Nomifensine and their glucose and IRI were evaluated in both conditions. Nomifensine inhibits basal IRI but does not inhibit IRI levels after an oral glucose challenge. It is possible that nervous dopaminergic endings are present at the level of B cells.

Adult↗
PubMed1980

[HbAlc in diabetic subjects in progressive deterioration of the metabolic situation].

Five subjects with type I and type II diabetes mellitus under worsening diabetic control were studied and their HbAlc levels were monitored over three months. There was a strong rise of HbAlc paralleling plasma glucose, while serum cholesterol and triglyceride levels do not rise significantly. These findings confirm that the glycosylation of haemoglobin in vivo reflects the mean blood glucose levels over the previous few weeks and suggest that the trouble of lipid metabolism in diabetes is a slower and more indirect expression of the derangement in diabetic control than HbAlc.

Blood Glucose↗