Biomedical subjects

I Tripatzis

Publications and source records attributed to I Tripatzis.

At least 19 recordsLinked to original sources

PubMed1987

Solid-phase antigen immunoassay for the detection of anti-zona pellucida-antibodies in clinically defined sera.

A new solid-phase antigen immunoassay was developed for the detection of anti-zona pellucida-antibodies. The assay was validated in a double blind study with clinically defined sera. The new assay is easy to perform and large numbers of sera can be processed in one day. The antigen can be prepared in advance and stored until use. This represents an advantage over other methods, such as immunofluorescence and RIA, which require fresh antigen preparation for each set of assays. With this new test ca. 7% of females and ca. 20% of males with unexplained infertility have shown elevated antibody concentrations compared with fertile controls. The estimated intra-assay and inter-assay CV were 8.5% and less than 10% respectively.

Antibodies↗

PubMed1978

Epidemiology of hepatitis B surface antigen (HBsAg) and antibody to HBsAg in hospital personnel.

The epidemiology of hepatitis B in hospital personnel was studied by testing of sera from 3,770 employees of the Medical School of Hannover (Hannover, West Germany) for hepatitis B surface antigen (HBsAg) and its corresponding antibody (anti-HBs) by solid-phase radioimmunoassay. An average prevalence of 2.2% for HBsAg and 11.7% for anti-HBs was found. Physicians (18.2%), nurses (20.1%), and members of the cleaning service (26.3%) showed the highest frequencies of HBsAg or anti-HBs carriage. In a study of age- and sex-matched personnel, nurses showed a significantly (P less than 0.01) higher rate of infection than a control group with less exposure to infectious materials. The frequency of HBsAg or anti-HBs was highest in persons associated with dialysis (31.3%), anesthesiology (31.0%), ophthalmology (29.4%, neurosurgery (28.0%), and surgery (24.4%). The rate of infection was significantly higher in surgical departments (24.4%) than in nonsurgical ones (13.3%). Persons who had been nursing patients with hepatitis were significantly (P less than 0.05) more frequently carriers of HBsAg or anti-HBs than a comparable control group.

Adult↗

PubMed1978

[Study on the effect of formaldehyde on the immunological reactivity and morphology of hepatitis B-antigen (author's transl)].

Treatment of purified HB-antigen with formaldehyde solution changes its immunological reactivity and its morphology. A decrease in antigen activity by formaldehyde treatment could be demonstrated by radial immunodiffusion and by radioimmunoassay (Fig. 1). Electron microscopic studies revealed a segmentation of tubular HBAg particles into small round forms (Fig. 3) and a shift of the ratio tubular forms: spherical forms in favour of the spherical particles (Table 1). These findings support the hypothesis that 20 nm particles originate from a division of tubular forms. Round forms became smaller after formaldehyde treatment (Fig. 4). The treatment with formaldehyde changed neither the density of HBsAg testes in CsCl gradients, nor the immunologic identity tested in immunodiffusion tests (Fig. 2). All prescribed effects were observed only in the high concentrations of formalin (10 and 1%), whereas in the concentration of 0.1%, which is usually used for inactivations in the development of vaccines, no immunological and morphological changes of the HBAg could be seen.

Densitometry↗

PubMed1978

[Radioimmunological differentiation of yeasts (author's transl)].

A microtiter solid phase radioimmunoassay is described for the differentiation or microorganisms by using six species of yeasts as a model. Rabbits were immunized with homogenized cells of the yeasts. The rabbit sera obtained were absorbed to homologous cells and after elution, the antibodies so obtained were labeled with 125I. Cross-reacting antibodies were eliminated by absorption to the corresponding yeast cells (table 1). With this radioimmunoassay it was possible to identify 51 out of 52 strains of C. albicans, C. tropicalis, C. pseudotropicalis, C. krusei, Torulopsis glabrata and Geotrichum candidum (table 2), whereas only 30 out of 52 strains could be differentiated by the conventional agglutination procedure (table 3). In the radioimmunoassay four immunoglobulin preparations directed against C. tropicalis, C. krusei, Torulopsis glabrata and Geotrichum candidum reacted only with the corresponding species. The immunoglobulin preparation directed against C. albicans reacted both with C. albicans and C. tropicalis and the preparation directed against C. pseudotropicalis reacted with C. pseudotropicalis and C. krusei (fig. 1). None of 60 isolates of 10 species of bacteria cross-reacted with the six yeast antisera, when tested in the radioimmunoassay.

Antibody Specificity↗

PubMed1976