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Biomedical subjects

I Tsuyuguchi

Publications and source records attributed to I Tsuyuguchi.

At least 55 records · Page 3Linked to original sources
PubMed1982

In vitro tuberculin reactivity of lymphocytes from patients with tuberculous pleurisy.

Mononuclear cells in pleural fluid from patients with tuberculous pleurisy were predominantly T cells. Responsiveness of pleural fluid T cells to purified protein derivative of tuberculin were studied by the assay of cell proliferation and production of lymphocyte mitogenic factor by the stimulation with purified protein derivative. Peripheral blood lymphocytes were also studied from patients and tuberculin-positive healthy controls. The order of responsiveness was as follows: pleural fluid lymphocytes greater than peripheral blood lymphocytes of patients without effusion = peripheral blood lymphocytes of healthy controls greater than peripheral blood lymphocytes of patients with effusion. The poor response of peripheral blood lymphocytes from pleurisy patients were recovered by the elimination of adherent cells in peripheral blood lymphocytes to the level of the response of peripheral blood lymphocytes from healthy controls. T cells purified from pleural fluid mononuclear cells responded more than those from peripheral blood. These results suggested that in the pleurisy patients purified protein derivative-reactive T cells in peripheral blood did not decrease in activity, but were depressed by suppressor cells, and further suggested that highly purified protein derivative-reactive T cells were accumulated in the pleural fluid.

Adult↗
PubMed1982

Nonspecific recruitment of lymphocytes in purified protein derivative-induced lymphocyte proliferative response of patients with tuberculosis.

Peripheral blood lymphocytes (PBL) from tuberculosis patients were studied for their in vitro proliferative response stimulated with purified protein derivative (PPD)-tuberculin. Studies were designed to characterize the lymphocytes involved in the PPD-induced proliferation. PPD-responsive lymphocytes were eliminated in PBL by the procedure of cultivation of PBL with PPD in the presence of 5-bromodeoxyuridine and light illumination of the cultured cells. These PBL which lost PPD reactivity were no longer able to proliferate to PPD stimulation but were still capable of proliferation in the presence of both PPD and X-irradiated, autologous fresh PBL or upon addition of culture fluids from PPD-stimulated PBL. In addition, these nonspecifically activated lymphocytes released a soluble factor into the culture fluids which inhibited the migration of leukocytes. It was likely that large numbers of nonspecific T cells were induced to proliferate as a result of the presentation of specific T cells with the antigen PPD. It is suggested that a similar recruitment of lymphocytes by PPD-stimulated T cells takes place in vivo during the establishment of tuberculosis or antituberculous immunity or both.

Bromodeoxyuridine↗
PubMed1982

Increase in rosette-forming T cells with autologous human erythrocytes in lymphocytes of patients with tuberculosis by in vitro stimulation with purified protein derivative.

Lymphocytes of tuberculous patients activated in vitro with purified protein derivative of tuberculin (PPD) were examined for rosette formation with human autologous erythrocytes. The percentage of the rosette-forming cell (auto-RFC) was increased when pleural fluid lymphocytes of patients with tuberculous pleurisy and peripheral blood lymphocytes (PBL) from patients with tuberculosis or from tuberculin skin test positive healthy individuals were stimulated with PPD in vitro, whereas no increase of auto-RFC was observed in PBL from tuberculin skin test negative donors. Increased numbers of auto-RFC were shown to form rosettes with sheep erythrocytes but have no IgG Fc receptors on their surfaces. It was also shown that adherent cells were required for the PPD-induced increase of auto-RFC. Depletion of PPD-induced auto-RFC by the density gradient sedimentation technique led to a significant decrease in the degree of the proliferative response to gradient sedimentation technique let to a significant decrease in the degree of the proliferative response to PPD and the number of the auto-RFC after stimulation with PPD. These findings strongly suggest that PPD-induced auto-RFC represents the consequence of an immunological interaction between sensitized T lymphocytes and relevant antigen PPD, and reflects the PPD responsiveness of tuberculosis at the T cell level.

Cell Adhesion↗
PubMed1982

A complete selective C1q deficiency in a patient with discoid lupus erythematosus (DLE).

A 32 year old male patient with discoid lupus erythematosus (DLE) was found to have a complete, selective deficiency of C1q subcomponent of the complement assessed either by haemolytic assay or by protein determination. Addition of highly purified C1q completely restored the complement haemolytic activity of the patient's serum. Neither C1q precipitin nor anti-complementary activity was detected. Lymphocytes isolated from the patient's peripheral blood, however, bound as many C1q molecules as those of healthy control individuals. The patient is in good health except for skin lesions. A low level of circulating immune complexes was detected in his serum, but no C1q molecules were bound. Serum complement activities of the patient's mother and two other siblings were within the normal range. An impaired synthesis of C1q protein was strongly suggested as the cause of C1q deficiency in this patient's serum.

Adult↗
PubMed1981

Tuberculin purified protein derivative-reactive T cells in cord blood lymphocytes.

Lymphocytes obtained from cord blood of newborn babies who were born of healthy mothers were studied in vitro for their responsiveness to purified protein derivative (PPD) of tuberculin. Cord blood lymphocytes proliferated in vitro by stimulation with PPD, despite wide variations in the results. Studies with fractionated lymphocytes revealed that PPD-responding cells belonged to E-rosetting, nylon wool-nonadherent T lymphocytes. Non-E-rosetting B lymphocytes alone did not proliferate at all after stimulation with PPD. In addition, bromodeoxyuridine and light treatment of in vitro PPD-stimulated lymphocytes eliminated the responsiveness to PPD. These results suggest that T lymphocytes do exist in cord blood and respond in vitro to stimulation with PPD. A possible role for PPD-reactive T lymphocytes in cell-mediated protective immunity in newborns is discussed.

B-Lymphocytes↗
PubMed1981

Human helper T cell factor(s) (ThF). I. Partial purification and characterization.

T cells obtained from pleural effusion of patients with tuberculous pleurisy were stimulated in vitro with PPD. The culture supernatant was shown to be substituted for T cells in PWM-induced IgG production by human B cells. As many as 0.5 to 3 x 10(9) lymphocytes were obtained from 1 patient, and about 85% of them were E-rosette positive, making it possible to purify human helper T cell factor(s) (ThF). Helper activity was sensitive to heating at 70 degrees C for 5 min and treatment with trypsin. ThF did not have conventional Ig determinants and was recovered in the 50 to 67% ammonium sulfate fraction. The purification was done by successive DEAE-Sephadex and CM-Sephadex ion-exchange chromatography, Sephadex G-100 gel filtration, and isoelectric focusing. It was shown that ThF activity was eluted by gel filtration in the fraction with a m.w. of about 20,000. Isoelectric focusing of this fraction revealed that ThF activity distributed in the pl range of 6.5 to 8.0, whereas TCGF activity was focused in a single peak at pI of 6.5, indicating the existence of a helper factor without TCGF activity.

Amylases↗
PubMed1981

In vitro induction of HLA-restricted cytotoxic T lymphocytes against autologous Epstein-Barr Virus transformed B lymphoblastoid cell line.

Cytotoxic T lymphocytes (CTL) against autologous EBV-transformed B lymphoblastoid cell line (LCL) were induced in vitro by culturing peripheral blood lymphocytes (PBL) of healthy donors together with mitomycin C-treated autologous LCL for 6 days. The cytotoxic cells developed only from the E-rosette-positive fraction but not from the negative fraction of PBL. These CTL killed autologous LCL but not PWM-stimulated autologous PBL. In addition, the CTL killed allogeneic LCL when at least 1 of the HLA-A antigens was identical with that of the LCL of CTL donor. However, identity of HLA-B and HLA-C antigens was not enough for a significant killing of allogeneic LCL. The specificity of the CTL was also confirmed by a cold target inhibition test. These results indicated that the CTL induced specifically recognized EBV-transformed cells with HLA restriction.

Animals↗
PubMed1980

Establishment of autoantibody-producing cell lines from peripheral blood lymphocytes of patients with systemic lupus erythematosus.

Autoantibody-producing B cell lines were established from peripheral blood lymphocytes of patients with systemic lupus erythematosus. Peripheral blood lymphocytes obtained from five of seven patients were successfully transformed by Epstein-Barr virus. Two of four established B lymphoblastoid cell lines examined in this study produced anti-nuclear factor antibodies and one of them produced anti-single-stranded DNA and anti-double-stranded DNA antibodies. These results indicate that B cell clones committed to self antigens are transformed by Epstein-Barr virus and continue to produce autoantibodies. In order to establish a monoclonal autoantibody-producing B cell line, the cells were cloned by a limiting dilution method. The data suggest that it is possible to establish a monoclonal autoantibody-producing B cell line by the combination of transformation of B cells by Epstein-Barr virus and extensive cloning.

Adolescent↗
PubMed1980

Depressed immunological defence mechanisms in mice with experimentally induced diabetes.

Persistent diabetes mellitus with marked hyperglycemia was induced in mice by the administration of streptozotocin. In these streptozotocin-induced diabetic mice, resistance to tubercle bacillus challenge and primary as well as secondardy humoral immune responses against foreign erythrocytes were markedly depressed. The T-cell function in delayed hypersensitivity to 2,4-dinitro-1-fluorobenzene and bacterial phagocytic activity or peritoneal macrophages were markedly depressed. In contrast, the B-cell function in antibody production against T-independent antigen and the intracellular killing of bacteria in peritoneal macrophages were intact. We concluded that depression of the T-cell function or the phagocytic activity of macrophages or both may be the main immunological defect in these mice.

Animals↗
PubMed1980

Increase in T cells bearing IgG Fc receptors in peripheral blood of patients with tuberculosis by in vitro stimulation with purified protein derivative.

A mixed rosette technique with sheep erythrocytes and chicken erythrocytes coated with heat-aggregated human IgG was used to identify human peripheral blood T lymphocytes bearing IgG Fc receptors (FcR+-T). When peripheral blood lymphocytes from patients with advanced, refractory tuberculosis were stimulated with purified protein derivative (PPD) in vitro, the number of FcR+-T cells increased. No FcR+-T cells developed in peripheral blood lymphocytes from patients with newly diagnosed tuberculosis after stimulation with PPD. The FcR+-T cells from a person who reacted positively to a PPD skin test were isolated by the method of velocity sedimentation. These cells suppressed the PPD-induced proliferative response of autologous peripheral blood lymphocytes as well as the pokeweed mitogen-induced IgG synthesis by B-cells.

Adult↗
PubMed1979

Regulatory mechanism of autoantibody production in mice to bromelin-treated isologous red blood cells.

In the spleen of mice immunized with bromelin treated rat red blood cells (RBC), the number of PFC against bromelin treated isologous RBC increased, although immunization with non-treated rat RBC or bromelin treated isologous RBC gave no increase in number of these PFC. This immune response was found to be T-independent and the PFC developed are exclusively of direct or Ig-M type. In the secondary immune response, production of these PFC was depressed rather than increased. This can be thought of as one of the defence mechanisms against overproduction of autoantibodies in confrontation to foreign antigens cross-reactive with self antigenic determinants. This depressed secondary immune response can be adoptively transferred by primed spleen cells but no active suppressor effect was found. We concluded that clonal elimination by exhaustive differentiation may be operative in this depressed secondary immune response.

Animals↗
PubMed1975

Local, passive transfer of purified protein derivative-delayed tuberculin hypersensitivity by guinea pig thymus-dependent lymphocytes.

Delayed skin reactions to purified protein derivative could be produced in guinea pigs by passive transfer of the population of sensitized lymphocytes, which have the ability to form rosettes with rabbit erythrocytes. Because it has been shown earlier that guinea pig thymus-dependent lymphocytes rosette with rabbit erythrocytes, it is concluded that thymus-dependent lymphocytes mediate delayed type hypersensitivity.

Animals↗