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Biomedical subjects

I Uematsu

Publications and source records attributed to I Uematsu.

At least 19 recordsLinked to original sources
PubMed1992

Cellulose acetate electrophoretic determination of bone alkaline phosphatase activity in healthy subjects and diabetic patients with and without osteopenia.

We adapted the electrophoretic method of bone alkaline phosphatase (ALP) determination using neuraminidase from Vibrio cholerae to separate bone and liver ALP on cellulose acetate membrane. Treatment of separator plus serum (1:8, neuraminidase 111 U/l in final) for 10 min at room temperature (25 +/- 1 degree C) and subsequent electrophoresis made it possible to quantify bone ALP activity simply and rapidly. The precision of the data was at the level of CV of 1.6% (within-day) and 4.7% (day-to-day), with recovery rates of 97-103%. The normal range of bone ALP activity depended on age and sex. Seventy-eight diabetes mellitus (DM) patients, excluding those with renal failure, were divided into two groups of those with and without osteopenia with matching of age (+/- 3 years) and sex. Bone ALP (P < 0.001) and total ALP (P < 0.05) activities and urine calcium/creatinine ratio (P < 0.05) were significantly higher in DM with osteopenia than in DM without osteopenia. Therefore, bone formation and absorption may be accelerated in DM with osteopenia in comparison with DM without osteopenia.

Adult↗
PubMed1990

[Biochemical analysis of bone and mineral metabolism in liver cirrhosis].

We investigated the disorder of bone and mineral metabolism in 29 patients with liver cirrhosis who were classified into two subgroups with (group 1, n = 13) or without (group 2, n = 13) osteopenia according to the method of Jikei. Serum levels of osteocalcin level, bone-type alkaline phosphatase activity and calcium concentration in serum and urine in these patients and 25 normal control subjects were determined. Serum osteocalcin level and bone-type alkaline phosphatase activity were elevated in group 1 compared with those in group 2 and normal control subjects. Serum and urine calcium levels in group 1 were similar to those in group 2. However calcium-creatinine ratio in urine was higher in group 1 than in group 2. These present observations indicate that osteopenia in patients with liver cirrhosis is due to high turnover of bone metabolism.

Adult↗
PubMed1985

[The disorder of vitamin D metabolism in patients with liver cirrhosis].

Liver cirrhosis (LC) is often associated with osteomalacia and osteoporosis. Since it has been shown that serum levels of 25 hydroxy vitamin D (25-OH-D) are reduced in LC, defective hepatic hydroxylation of vitamin D has been postulated to be responsible for the low serum 25-OH-D levels and skeletal demineralization. This study was designed, therefore, to determine serum 25-OH-D and 1 alpha, 25-(OH)2-D levels in patients with LC. Further, the response of serum 1 alpha, 25-(OH)2-D to a single oral dose of 1 alpha-OH-D3 (2 micrograms) was investigated. In 5 patients with severe decompensated LC and 3 patients with compensated LC, serum 25-OH-D and 1 alpha, 25-(OH)2-D levels were respectively measured by the modified method of Belsey and by that of Eisman. Serum 25-OH-D in patients with compensated and decompensated LC was significantly higher than that in normals. Serum levels of 1 alpha, 25-(OH)2-D in patients with decompensated LC were significantly lower than those in patients with compensated LC and normals. After a single oral administration of 1 alpha-OH-D3 at a dose of 2 micrograms, the 1 alpha, 25-(OH)2-D rose in each patient within 6h, reaching the maximum levels at 12h. The percent increase over the basal value in decompensated LC was similar to that in compensated LC.(ABSTRACT TRUNCATED AT 250 WORDS)

Dihydroxycholecalciferols↗
PubMed1983

Evaluation of biliary pressure by perfusion method and its significance.

The present authors have made it a rule to use a modified Mellet-Guy's method for measuring pressure in the bile duct. The determined manometric pressure expressed in curve has been classified into (a) basal pressure, (b) rising time, (c) perfusion pressure, (d) dumping time and (e) residual pressure. The basal pressure (a) represents a static pressure free from any load in the bile duct. The rising time (b) varies with factors including the measuring apparatus, ductal elasticity and capacity, resistance at the choledochal terminal, and the volume of physiological saline infused. The perfusion pressure (c) is likely to undergo interference of a variety of factors such as the ductal diameter, mural elasticity and thickness, resistance at the choledochal terminal, along with the measuring apparatus. The dumping time (d), free from the influence of the measuring apparatus, is considered to be an important guide for us to guess the condition of choledochal terminal. The residual pressure (e) and the trend of manometric pressure curve help us to surmise the choledochal terminal resistance and the presence of ductal abnormalities. After all, among the above factors, the dumping time and the residual pressure are apparently available for objective evaluation of bile duct pressure.

Bile Ducts↗
PubMed1981

Follow-up studies of surgical treatment for choledocholithiasis.

The follow-up results of cholecystectomy and common duct exploration with T-tube drainage for 308 patients with common bile duct stones were studied. Nine patients evaluated as "poor" included seven with residual stones and two with recurrent stones.. Cholangiography at the time of follow-up manifested the evidence of a statistically significant reduction of the bile-duct diameter compared with the preoperative level in both cholesterol and calcium bilirubinate cases. The reduction was particularly conspicuous in the group with marked dilatation of the bile duct, regardless of the types of stone. Of the 134 patients with cholelithiasis who underwent reoperations in our Department, 75 (87%) belonged to residual, 11 (13%) to recurrent group. Of the 11 patients with recurrent stones, nine had obvious causes for stone recurrence such as bile-duct stenosis or stricture at the anastomotic site. These results suggest that without existence of evident stenosis at the papilla of Vater or in the lower bile duct, patients with choledocholithiasis, regardless of types of stone, could be curable effectively only by cholecystectomy and choledocholithotomy with T-tube drainage.

Adult↗
PubMed1980

Surgical management of intrahepatic gallstones.

The results of surgical treatment in 100 patients with intrahepatic gallstones are reported including follow-up on 61 patients who survived more than three years. Nine patients died in the follow-up period, and of the remainder, 44 showed complete rehabilitation (72%). The results of follow-up examinations were best shown by improvement in the tests of hepatic function when compared with the values at the time of discharge. However, in those patients with a long duration of illness, or with residual stones or with impairment of liver function, many claimed persistent complaints and showed abnormal late values of hepatic function. Patients with intrahepatic gallstones are best managed: 1) removal of all gallstones as thoroughly as possible, 2) additional procedures to eliminate bile stasis and permit residual stones to enter the intestinal tract and 3) irrigation of the biliary ducts to remove remaining stones.

Adult↗