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Biomedical subjects

I Uno

Publications and source records attributed to I Uno.

82 records · Page 5Linked to original sources
PubMed1976

Effect of cyclic AMP on glycogen phosphorylase in Coprinus macrorhizus.

Glycogen phosphorylase (1,4-alpha-D-glucan:orthophosphate alpha-glucosyltransfase, EC 2.4.1.1) activity was found in mycelial extracts of Coprinus macrorhizus concurrently with decrease of glycogen content in mycelial cells. Incubation of the enzyme sample with cyclic AMP and ATP leads to a 3-fold activation of the glucogen phosphorylase activity. Activation of the enzyme partially purified through Sepharose 6B required a cellular fraction containing cyclic AMP-dependent protein kinase.

Adenosine Triphosphate↗
PubMed1976

Differences in properties of cytosol and membrane-derived protein kinases.

Adenosine 3':5'-monophosphate-dependent protein kinase present in membrane fractions of bovine brain, heart, liver, and muscle was solubilized with Triton X-100. Certain properties of the membrane-derived enzyme were compared with those of two adenosine 3':5'-monophosphate-dependent protein kinases present in the cytosol fractions from each of the same tissues. The properties studied included chromatographic behavior on DEAE-cellulose columns, specificity with respect to substrate proteins, and sensitivity to NaCl and Triton X-100. The membrane-derived enzyme from each tissue had properties similar to those of the membrane-derived enzyme from each of the other tissues. Moreover, the cytosol enzymes from each tissue had properties similar to those of the corresponding enzymes in the cytosol from each of the other tissues. However, for any given tissue, the properties of the membrane-derived enzyme differed from those of the cytosol enzymes, possibly reflecting different functional roles for the membrane-bound and cytosol enzymes.

Animals↗
PubMed1974

The effect of light on fruiting body formation and adenosine 3':5'-cyclic monophosphate metabolism in Coprinus macrorhizus.

The monokaryotic mycelia of a mutant strain, fis(c), of Coprinus macrorhizus, which are able to form monokaryotic fruiting bodies in the light, failed to form any fruiting bodies in darkness. A dikaryon and a mutant strain, ds, formed malformed fruiting bodies in darkness. Illumination for 1 day of fis(c) mycelia grown in darkness for 4 days or longer was effective in inducing malformed fruiting bodies. The accumulation of adenosine 3':5'-cyclic monophosphate in the illuminated mycelia of strain fis(c) was demonstrated. The illuminated mycelia of strain fis(c) produced high levels of adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] and phosphodiesterase (3':5'-cyclic-AMP 5'-nucleotidohydrolase, EC 3.1.4.17), which degrades cAMP, while the dark-grown mycelia showed no or very low activities of these enzymes. Dikaryotic mycelia and monokaryotic mycelia of strain ds produced significant amounts of these enzymes even in darkness. When the dark-grown mycelia of strain fis(c) were exposed to continuous light, the activities of adenylate cyclase and phosphodiesterase increased rapidly after a lag period whose length depends on the culture age of mycelia. Cycloheximide inhibited the increase in these enzyme activities stimulated by light. When the fis(c) mycelia were exposed to continuous light, an increase in cAMP-binding activity was observed. A possible participation of cAMP in the formation of fruiting bodies in C. macrorhizus is discussed.

Adenylyl Cyclases↗
PubMed1974

Effect of glucose on the fruiting body formation and adenosine 3',5'-cyclic monophosphate levels in Coprinus macrorhizus.

The formation of fruiting bodies in the monokaryotic fis(c) strain and a dikaryon of Coprinus macrorhizus was inhibited by growth in high-glucose media. In high-glucose media the characteristic burst of adenosine 3',5'-cyclic monophosphate (cAMP) accumulation during fruiting-body formation was absent. Enzymatic activity assays revealed that mycelia grown in high-glucose media contained relatively lower amounts of adenylate cyclase and cAMP-phosphodiesterase than mycelia grown in low-glucose media. The synthesis of inducible d-serine deaminase and tryptophanase was repressed in high-glucose media. A mutant (gluR) in which the glucose repression of fruiting-body formation is affected was isolated by selection in high-glucose media. The mutation caused the cAMP levels to be no longer affected by glucose and affected ability to synthesize the inducible d-serine deaminase and tryptophanase. The gluR mutant was partially dominant in dikaryons. It is suggested that cAMP may play important roles in inducing fruiting bodies and in controlling inducible enzyme synthesis in C. macrorhizus.

Adenylyl Cyclases↗
PubMed1973

Purification and identification of the fruiting-inducing substances in Coprinus macrorhizus.

Substances which are effective in inducing fruiting bodies in monokaryotic mycelia of the fis(+) strain of Coprinus macrorhizus were purified and characterized. The active components of fruiting-inducing substances were identified as adenosine-3'-monophosphate, adenosine 3',5'-cyclic monophosphate (cyclic AMP), and a protein which is bound with the cyclic AMP. Cyclic AMP was synthesized from adenine within mycelia of the mutant strains which form monokaryotic fruiting bodies without the addition of fruiting-inducing substances, but not in those of the strains which do not form monokaryotic fruiting bodies. The proteins which bind with cyclic AMP were detected in crude extracts of mycelia of those strains which form monokaryotic fruiting bodies and of the dikaryon, but not in those of the strains which do not form monokaryotic fruiting bodies.

Adenine↗
PubMed1973

Metabolism of adenosine 3',5'-cyclic monophosphate and induction of fruiting bodies in Coprinus macrorhizus.

The adenyl cyclase and phosphodiesterase metabolizing adenosine 3',5'-cyclic monophosphate (cyclic AMP) were detected in mycelia of strains of Coprinus macrorhizus which form fruiting bodies, but not in those of strains which do not form fruiting bodies. The adenyl cyclase synthesized cyclic AMP from adenosine triphosphate. The phosphodiesterase degr[UNK]ded cyclic AMP to adenosine-5'-monophosphate and was inhibited by adenosine-3'-monophosphate, theophylline, and caffeine. The strains which form fruiting bodies incorporated and metabolized cyclic AMP, but strains which do not form fruiting bodies did not. The possible participation of cyclic AMP in the induction of fruiting bodies is discussed.

Adenosine Monophosphate↗
PubMedDate not supplied

Analysis of stress distribution in a screw-retained implant prosthesis.

Four types of implant superstructures were screwed onto implant bodies, and the strains created around the implant bodies were compared and analyzed within the IMZ Implant System. Three IMZ implants were embedded in the center of a polyurethane block (30 x 40 x 30 mm), and a total of 16 superstructures was fabricated by 4 methods: 1-piece cast, 1-piece cast/split soldering, soldering, and passive fit. Six strain gauges were placed on the surface of the block 1 mm apart. Three embedded implants were numbered, and a fixed partial denture was placed on these implants and screwed by a torque wrench using 14.5 Ncm torque. This procedure was repeated 7 times for each fixed partial denture, and each created strain was measured when the last screw was tightened. In all fixed partial dentures, strains were produced around the implant bodies when screws retaining the prosthesis were tightened, and the strain was relieved with unscrewing. The magnitude of strain was greater with the 1-piece cast method or the section/solder method than with the soldering and passive-fit methods. Of the 2 soldering methods, when the screw on the middle implant was tightened before those on the terminal 2 implants, the magnitude of strain was lower with the soldering method than with the 1-piece cast/split soldering method. When the order of screw tightening was changed, there were significant differences in the magnitude of strain at each gauge with the soldering method. With the passive-fit method, no differences in the magnitude of strain attributable to the order of screw tightening could be detected. The magnitude of strain produced around a screw-retained implant prosthesis was significantly lower with the passive-fit method when compared to the other 3 fabricating methods. Furthermore, the implants prepared by the passive-fit method were not affected by the order of screw tightening.

Analysis of Variance↗