[Evolution of physical methods of hemostasis in surgery].
Explore the source record for details and available documents.
Blood Loss, Surgical↗
Biomedical subjects
I V Aksenov
Publications and source records attributed to I V Aksenov.
[Ochratoxin A contamination of cereal grain harvested in 2003 and 2004 years].
The analysis of ochratoxin A--mycotoxin produced by widely distributed Aspergillus and Penicillium--of cereal grain harvested in 2003 - 2004 years were performed by immunoaffinity column clean-up and HPLC with fluorescence detection. This survey examined 282 samples of raw grain wheat, rye, barley and oat 13.8% of all samples contaminated by ochratoxin A in the range 0.2-33.3 mg/kg. Calculation made on the basis of the obtained means showed that the daily ochratoxin A intake of human from cereal grain were from 1.58 to 2.84 ng/kg b.w. Scientific Committee for Food of the European Commission suggested that it was prudent to reduce exposure to ochratoxin A as much as possible below 5 ng/kg bw/day. Codex Alimentarius and European Commission have established maximum permissible level of 5 mg/kg for ochratoxin A in raw cereal grains.
Aspergillus ochraceus↗
[Laser energy in abdominal surgery].
The results of 210 operations on the gastrointestinal tract with application of YAG-Nd laser have been analyzed. The role of laser in the improvement of surgical outcomes is demonstrated.
Abdomen↗
[The method of the simultaneous determination of the citrinin and ochratoxin A content in cereals and cereal products].
The simplifyed method of simultaneous determination of the citrinin (CT) and ochratoxin A (OTA) in cereals is described. The extraction of mycotoxins was carried out by small volumes of solvents (water/aceton/hexane) without additional sample clean up and concentration. The extracts were analysed by HPLC using mixture of methanol/ethylacetate/phosphoric acid, pH 2.2 as a mobile phase and fluorescence detection (lamda ex 330 nm, lamda em 495 nm). The reliability and reproducibility of results were improved by usage of internal standard--methyl-derivative of ochratoxin A. Average recovery of CT and OTA was 70%, relative standard deviation 12% and 7%, respectively, limit of detection 0.003 mg/kg.
Chromatography, High Pressure Liquid↗
[Ochratoxin A content in baby food].
The purpose of this study was to investigate the ochratoxin A content in baby food. The analysis of ochratoxin A were performed by immunoaffinity column clean-up and HPLC with fluorescence detection. The detection limit was 0.2 mg/kg. Ochratoxin A was detected in 22.5% of 40 samples up to 1.2 mg/kg. Mean level was 0.15 and 0.31 mg/kg. Ochratoxin A level was higher in oat-based samples. Calculations made on the basis of the obtained means showed that the daily ochratoxin A dietary intake were up to 1.72 ng/kg b.w.
Food Analysis↗
[Intrarenal hemodynamics in patients with systemic rheumatic diseases].
AIM: To study intrarenal hemodynamics (IRHD) in patients with systemic lupus erythematosus (SLE) and sclerodermia systematical (SS). MATERIALS AND METHODS: IRHD was studied in 30(28 females and 2 males) and 25(22 females and 3 males) patients with SLE and SS, respectively, and mean duration of the disease 5.6 +/- 0.5 and 7.5 +/- 1.2 years, respectively. Lupus nephritis was diagnosed in 21(70%), sclerodermic nephropathy in 10(40%) patients. Patients with chronic renal failure were not included. IRHD was investigated using protein loading which provided the index of functional renal reserve (FRR). RESULTS: Impaired IRHD was detected in 21(70%) patients with SLE. 19 patients had lupus nephritis. FRR depletion was found also in 2 patients free of lupus nephritis symptoms. 10 of them (8 with active nephritis and 2 with intact kidneys) had arterial hypertension. FRR depletion was found also in 12(48%) of SS patients. 7 of them had sclerodermic renal lesions. CONCLUSION: Impaired IRHD was encountered frequently both in SLE and SS patients and can develop in the absence of renal affection symptoms.
Disease Progression↗
[Hepatocyte cytolysis enzymes as the markers of operative trauma in open cholecystectomy].
Surgical and narcotic aggression lead to certain injuries of hepatocytes. The authors followed up the time course of liver injury marker enzymes in two groups of patients subjected to open cholecystectomy (n = 62): succinate dehydrogenase (SDH), glutamate dehydrogenase (GIDH), SGPT, and SGOT. In group 1 the operative trauma was minimal, in group 2 traumatism was higher because of technological difficulties. A high diagnostic significance of SDH and GIDH is worthy of note: their activities increase in proportion with the severity of surgical trauma. Comparison of laboratory data helps objectively assess the severity of operative injury in open cholecystectomy.
Biomarkers↗
[The correction of hypoxic and ischemic states with the use of antihypoxants (a review of the literature)].
The review of literature contains data concerning the highly efficient antihypoxant--"Hutemin". The article also describes the group of "standard antihypoxants" (Natrii oxydutyras, Pyracetam, Amtizol), and some perspective pharmacological drugs with antihypoxic activity (Trimin, Aethomerol, Perfluorine-carbonaceous emulsions) which actually undergo pilot clinical researches. Summarizing the data, the authors make a conclusion about the necessity to conduct search for new antihypoxants with various mechanisms of action on systemic and cell levels, and also to widen their clinical application.
Hemorrhage↗
[Effect of hyperoxia and anoxia on lymph flow from isolated limb vessels].
Explore the source record for details and available documents.
Animals↗
[Optimization of analytical methods for assay of ochratoxin A in the foodstuffs].
Ochratoxin A is a mycotoxin produced by widespread mold fungi of the genera Aspergillius and Penicillium. It is a common contaminant of cereals, coffee, wine, dried fruits, and spicery. There is evidence that ochratoxin A has nephrotoxic, immunosuppressive, embryotoxic, teratogenic, and carcinogenic effects on many species of mammals. Its maximum allowable concentration established by the Alimentarius Code and the European Union is 5 microg/kg for cereals and 3 microg/kg for ready-made cereals. Two optimized techniques (silica gel column chromatography (CC) or immunoaffinity CC for extract purification and high performance liquid chromatography with fluorescence detection) are proposed for the detection, identification, and assay of ochratoxin A in food commodities. The detection limit was 0.05 and 0.5 microg/kg, respectively. The developed techniques were used to assay the content of ochratoxin A in 46 samples of the grain gathered in different regions of Russia. Nine samples were contaminated by ochratoxin A at concentrations of 0.05 to 5 microg/kg.
Chromatography, Affinity↗