Biomedical subjects

I V Miroshnichenko

Publications and source records attributed to I V Miroshnichenko.

At least 19 recordsLinked to original sources

PubMed1992

[Effects of B-lymphocytes from different organs on hemopoietic colony formation in the spleen by bone marrow cells].

The influence of B-lymphocytes from various sources on splenic colony formation was studied in the syngeneic system. B-lymphocytes were obtained by panning with IgG-fraction of rabbit anti-mouse Ig, absorbed on Petri dishes. In addition, adherent cells, Thy-1+ and SC-1+ were eliminated from the fraction of Ig(+)-cells. SC-1- and SC-1+ fractions, containing, respectively, stem cells and T-lymphocyte precursors, were obtained by panning with IgG-fraction of rabbit anti-SC-1 serum. SC-1- cells transferred to irradiated syngeneic mice did not induce colony formation in the spleen. Introduction of SC-1- and SC-1+ cells induced formation of colonies. A similar helper effect occurred when SC-1(-)-cells were introduced with bone marrow or lymph node B-cells, but not with splenic B-cells. Splenic, but not bone marrow and lymph node B-cells inhibited colony formation by combination of SC-1- and SC-1+ cells. All effects of Ig+ cells were abolished by treatment of cells with rabbit anti-MBLA serum. Thus, B-cells of various origin can either enhance or inhibit colony formation. The enhancing of inhibitory effect after B (MBLA+)-cells elimination from suspension of bone marrow and lymph node (but not spleen) Ig(+)-cells resulted from the activity of B-contrasuppressors.

Animals↗

PubMed1990

Bone marrow and intrathymic precursors of T-cells produce a factor which enhances colony formation in the spleen.

Bone marrow Ig-Thy-1-SC-1- stem cells (precursors of T-lymphocytes, PTL, containing the SC-1 antigen) spontaneously secrete a humoral factor. When bone marrow Ig-Thy-1-SC-1- cells were treated with this factor they became able to form haemopoietic colonies in the spleens of lethally irradiated mice. This new colony-stimulating factor (CSF) is thermostable and has a molecular mass of about 25-30 kDa. Production of the CSF by bone marrow SC-1+Thy-1- cells was stimulated by treatment with the thymus preparation Thymoptin. Such treatment of bone marrow SC-1+Thy-1- cells also induced the production of a suppressor factor (thermolabile, molecular mass about 45 kDa), that reversed the effect of the CSF. The CSF and the suppressor factor are both also produced by cortisone-resistant radioresistant L3T4-Lyt-2-SC-1+ thymocytes (i.e. intrathymic PTL). The regulation of haemopoiesis appears to be a normal function of bone marrow PTL.

Animals↗

PubMed1988

[Assessment of the action of taktivin on different stages of T-lymphocyte maturation].

Tactivin, the thymic hormone preparation, evokes some phenotype alterations in T-cell precursors (elimination of SC-1 antigen and expression of Thy-1-antigen) and cortical thymocytes (a decrease in the number of thymocytes carrying PNA-receptor) similar to those arising in T-cell differentiation. Tactivin induces PNA+ -thymocyte response to PHA action and increases PHA response to PNA- -thymocytes. It is weakly mitogenic for T-cell precursors and PNA- -thymocytes. The data suggest that Tactivin may be used for the treatment of immune deficiencies with T-cell differentiation and function defects.

Adjuvants, Immunologic↗

PubMed1988

[Effect of ribonucleases on humoral immunity of experimental animals].

The stimulating effect of RNAases on the humoral immune response was observed in experiments with animals. It was shown that the stimulation was mainly mediated by the system of T-lymphocytes. In the T-lymphocyte system positive sensitivity to the enzymes was attributed to the T-helper cell subpopulation.

Animals↗

PubMed1986

[Migration of T-lymphocyte precursors into the thymus and the factors affecting this process].

Migration of FITC-labeled mouse bone marrow cells into the thymus was measured by flow cytometric analysis 3 hours after intravenous injection of cells into irradiated mice. The percentage of cells reaching the thymus diminished when the dose of injected cells increased. The dependence of the number or labeled cells in the thymus on the dose of injected cells was not linear. Pretreatment of cells with anti-SC-I serum, peanut lectin or H-2 incompatibility antigen abolished thymus migration, while treatment with anti-Thy-I serum, soybean lectin, trypsin or Thy-I-incompatibility antigen diminished cellular migration and treatment with neuraminidase enhanced it. It was concluded that the main type of migrating cells is SC-1+ precursors of T-lymphocytes. Penetration of these cells through the blood-thymus barrier is based on the recognition of their partly sialized surface glycoprotein receptors by membrane lectins of the blood-thymus barrier cells.

Animals↗

PubMed1983

[Nonharsh methods of isolation of antigenic complexes of Klebsiella pneumoniae, and analysis of their protective activity].

Protective antigenic complexes capable of protecting mice from septic Klebsiella infection can be obtained from Klebsiella pneumoniae. The preparations obtained by the method of aqueous extraction and by the action of hydroxyl amine and autolysis have been found to possess approximately the same protective activity and to protect mice from infection with Klebsiella pneumoniae strains belonging to both homologous and heterologous serotypes.

Animals↗

PubMed1975