[Biological characteristic of the main pathogens in purulent bacterial meningitis].
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Biomedical subjects
Publications and source records attributed to I V Vlasova.
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Two mathematical models developed on the basis of a discriminant analysis of the evidence obtained in examination of 84 patients with chronic obstructive bronchitis (COB) are proposed in early diagnosis of right ventricular hypertrophy (RVH) by ECG readings in COB patients. Application of hierarchic classification model is recommended for diagnosis of RVH in screening. The probability model is intended for follow-up of COB patients.
The aim of the study was to examine changes in vasodilatory endothelial function in early cerebrovascular disease of atherosclerotic genesis. All the examinees underwent ultrasonic investigation, test for reactive hyperemia of the brachial artery to evaluate vasodilatory function of the endothelium. Disturbances in the flow-dependent vasodilation were detected at initial stages of the disease, aggravated with the progress of cerebrovascular disease and therefore could serve the earliest, objective indicator of atherosclerotic process.
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One thousand and four hundred strains of V. cholerae 01 isolated in 1994 in the Ukraine were studied with respect to their antibiotic susceptibility and resistance. The study showed that it was possible not only to estimate the present tendencies in and the regularities of the change in their character but also to presuppose the probable circulation and incidence of the microbe based on the differences in the susceptibility, frequency and resistance pattern of the strains of V. cholerae 01 isolated from the environment and humans before and during the cholera outbreak. Unlike the strains of V. cholerae 01 isolated from the environment before the outbreak, the strains isolated during the outbreak from the environment and humans were characterized by resistance to levomycetin (chloramphenicol) and streptomycin. The results suggested that the cholera outbreak in 1994 was incidental. The data are useful for cholera epidemic surveillance. However, the final conclusion is possible after investigation of the gene type pattern in the circulating V. cholerae strains.
The concentrations of gentamicin and cefotaxime (claforan) in the humor of the anterior chamber and vitreous body of the eye were estimated in the study on the pharmacokinetics of the antibiotics in rabbits. The antibiotics were administered intravitreally in single doses. It was shown that the residence time of the antibiotics in the therapeutic concentrations in the eye cavity was 48 hours. Cefotaxime proved to be the most efficient agent in the prevention and treatment of postoperative endophthalmitis.
The antibiotic sensitivity of 696 cultures belonging to the family Vibrionaceae (V. cholerae O1, V. cholerae non-O1, V. albensis, V. parahaemolyticus, V. alginolyticus and Aeromonas spp.) was studied and general regularities of the antibiotic sensitivity were shown: a high sensitivity to broad-spectrum antibiotics (tetracycline and chloramphenicol) and a low sensitivity to ++beta lactams (carbenicillin and ampicillin). The comparative examinations revealed similarity in the antibioticograms of V. cholerae O1 (el Tor++), V. cholerae non-O1 and V. albensis, especially the latter two groups, as well as the tested halophilic Vibrio cultures by the range of the MICs, Mo, Me and the nature of the antibiotic resistance. Cultures of V. cholerae and luminescent Vibrio tended to preserve a high sensitivity. High resistance levels were noted in the halophilic Vibrio and Aeromonas cultures. No significant differences in the sensitivity of the strains of various origin (from man and environmental objects) were detected. However, several more resistant strains were isolated from the environmental objects.
The authors present the qualification requirements to laboratory physicians specialized in bacteriology. The knowledge and skills of a bacteriologist, necessary for his organizations, methodologic, prophylactic, and diagnostic activities, are listed.
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On the basis of the literature data and experimental findings approaches are proposed for improving the agar diffusion method for determination of microbial sensitivity to antibiotics involving a semiquantitative or quantitative system for interpretation of the diffusion data obtained with the use of a new nutrient medium.
A total of 206 strains of various staphylococcal species isolated from various sources were studied with respect to their sensitivity to 18 antibiotics. The number of strains poly-resistant to the antibiotics was almost the same among Staph. aureus and Staph. epidermidis, i. e. 54.8 and 51.3 per cent respectively. The coagulase-negative and mannitol-negative variants of Staph. aureus and Staph. epidermidis possessing high biological activity (10-14 properties) were resistant to more antibiotics as compared to the low active strains.
Seven antibiotics, such as gentamycin, kanamycin, streptomycin, polymyxin M, carbenicillin, tetracycline and rifampicin were studied with respect to their effect on 260 strains of Ps. aeruginosa isolated from various sources within 1945-1971. Gentamycin and polymyxin M proved to be most active in vitro. Carbenicillin showed moderate activity. The sensitivity levels of the cultures of Ps. aeruginosa isolated from various sources within 30 years were identical. Serological typing of 59 cultures of Ps. aeruginosa was performed and their virulence was studied in parallel with their antibiotic sensitivity testing. No correlation between the antibiograms, serotypes and virulence of Ps. aeruginosa was found.
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