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Biomedical subjects

I Voigt

Publications and source records attributed to I Voigt.

At least 19 recordsLinked to original sources
PubMed2000

Use of a plasmid of a yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors.

A plasmid with a size of 2,682 base pairs isolated from the Yersinia enterocolitica biogroup 1A strain # 29807 was characterized in respect to its suitability as a basic replicon for cloning vectors. The copy number of the plasmid was determined to be approximately 14 copies per cell and it was shown to be compatible with vectors with an origin of replication derived from ColE1 and p115A. The replication region of the plasmid encodes a primer RNAI and countertranscript RNAII. Two vectors, pIV1 and pIV2, containing a kanamycin resistance gene and the lacZalpha fragment with the multiple cloning site of pBluescriptSK + were constructed. A mobilizable derivative was successfully introduced into different bacteria belonging to the family Enterobacteriacea. To prove the applicability of the novel vectors for cloning purposes, a 13 kb hemolysin operon of Escherichia coli was inserted into pIV1, and the resulting recombinant plasmid was stably maintained and expressed in E. coli and Y. enterocolitica.

Base Sequence↗
PubMed2000

CXCR5-deficient mice develop functional germinal centers in the splenic T cell zone.

The chemokine receptor CXCR5 is thought to be essential for the migration of B cells into the network of follicular dendritic cells in the spleen. However, as shown here, B cells and follicular dendritic cells do co-localize, albeit aberrantly, even in the absence of CXCR5. In mice lacking CXCR5 both cell types are found in a broad ring around the sinuses of the marginal zones. Upon immunization with the T cell-dependent antigen 2-phenyl-oxazolone, ectopic germinal centers develop in the periarteriolar lymphocyte sheath. A network of follicular dendritic cells forms in the vicinity of the central arteriole within which the antigen-activated B cells proliferate. The analysis of the expressed V gene repertoire revealed that during B cell proliferation, hypermutation is activated and V region genes accumulate somatic mutations. The pattern of somatic mutations suggests that affinity selection may occur. This analysis confirms that in CXCR5-deficient mice, the organization of splenic primary follicles is severely impaired. However, within the T cell zone a micro-environment is built up, which provides all requirements needed for the affinity maturation to take place.

Animals↗
PubMed1992

[Special characteristics of physician involvement in AIDS counseling].

Characteristic features of medical work at an AIDS Information Centre are pointed out with particular regard to the present situation in the city and district of Crenter? Hannover. Persons infected with HIV are reviewed, drawing particular attention to the extreme stress of living with the knowledge of being HIV-infected. The arising social problems of HIV-positive patients are described together with the doctor's adviser of easing the patient's stress and guiding him into accepting the necessary changes in life. Intensive cooperation hospital doctors, GP's and social workers is described.

Acquired Immunodeficiency Syndrome↗
PubMed1992

[Medical management of HIV infected and AIDS patients in the Hannover metropolitan area as an interdisciplinary construct].

Two wards for consultation of AIDS have opened in Hannover in 1987, one in the Public Health Department for the city of Hannover, the other in the Public Health Department for the surroundings. It was a big step towards prevention of AIDS. At the same time a centre, especially for AIDS, was opened at the Medical college of Hannover, consisting of an ambulance and beds in a ward. An official laboratory tests the medical analyses. Today the doctor in the Public Health Department plays an important part: he is the first partner with whom the infected patient can talk. He must also maintain contact with all institutions including the family doctor for optimum patient care.

Acquired Immunodeficiency Syndrome↗
PubMed1989

[10 years of dental care of hemophiliacs].

Increased consciousness of oral hygiene, improvement of sanitation degree and decrease of the number tooth extractions have been proved a success and an expression of high compliance within the scope of long-term interdisciplinary care of haemophilic patients. At the same time both patients registration and concentration at an out-patient department facilitate cooperation with the paediatricians and internal specialists being in charge of them, in addition a change-over from children to adult dispensary care is ensured. Haemophilic patients suffering from hepatitis B or HTLV-III/HIV antibodies carrier may be registered immediately. All patients undergoing dispensary care are subject to preventive test obligations.

Adult↗
PubMed1988

[The influence of different fiber types on the fecal nitrogen excretion and the content of volatile fatty acids in the feces of broiler hen breeds].

4 colostomized adult broiler breeding hens each received 120 g mixed feed per day. In addition, the animals were given 10, 20, 30 and 40 g/day cellulose powder or apple pectin resp. and 10, 20, 30 g/day straw cellulose. The DM content of the faeces was increased by cellulose powder and decreased by pectin (P less than 0.01) in comparison to the control group. DM excretion in g/animal and day increased more quickly after supplementing cellulose powder than after supplementing pectin. Straw cellulose produced results in between. The fibre supplements increased N excretion in faeces. It was, however, only significantly increased per 100 g DM intake by large pectin supplements (greater than 20 g). The TCA soluble N quota in the total N of the faeces remained largely unchanged after pectin supplements and increased after cellulose and straw cellulose supplements. Pectin supplements significantly increased the formation of acetate, propionate and butyrate in comparison to the other groups. Propionate could sporadically be detected in the faeces of the other test animals, butyrate not at all. In combination with 40 g apple pectin/animal and day, Bisergon (chinoxalin derivative) increased the formation of the 3 volatile fatty acids significantly.

Animal Feed↗
PubMed1979

[Critical evaluation of the results of systematic pedodontic care].

The authors present and comment upon the clinical and radiological findings obtained from 1200 children in the framework of a caries-statistical cross-sectional study. The statistical data are subdivided into: state of oral rehabilitation, extent of oral rehabilitation, frequency of the different kinds of filling and endodontic treatment measures.

Adolescent↗
PubMed1979

[Efficiency criteria and priorities in school age pedodontic care].

On the basis of findings in 1200 individuals aged between 7 to 16 years, the authors indicate priorities of dental care in the different age-groups. Apart from other criteria, the readiness for treatment and the disciplined behaviour of the children and adolescents during treatment may be used for assessing the dentist's achievements.

Adolescent↗
PubMed1979

[Pharmacokinetic studies on broad spectrum antibiotic combinations of penicillin and oxacillin in normal volunteers (author's transl)].

After i.v. application of broad spectrum penicillins (ampicillin, mezlocillin, carbenicillin) alone or combined with oxacillin (concomitantly) in different dosages to normal volunteers, the course of serum levels and recovery from urine were assessed and pharmacokinetic data were calculated for a 1-compartment model, in some cases also for a 2-compartment model. The following penicillins were tested alone and combined with oxacillin; ampicillin 3 g, oxacillin 2 g and their combination; ampicillin 2 g, oxacillin 1 g and their combination; mezlocillin 3.75 g, oxacillin 1.25 g and their combination; mezlocillin 2 g, oxacillin 1 g and their combination; carbenicillin 7.5 g, oxacillin 2.5 g and their combination. Apart from one combination (oxacillin 2 g + ampicillin 3 g), all penicillin combinations exceeded the serum levels achieved after individual application at any time. In combinations, the elimination constants decreased, total clearance was lower, and half-lives increased. The distribution volumes varied. With one exception, oxacillin 2.5 g + carbenicillin 7.5 g, all the combinations yielded a higher recovery from urine than did individual administration. When administering i.v. two penicillins simultaneously, a process similar to that following administration of probenecid in association with caronamide must need be considered. It may be assumed that the prolongation and elevation of serum levels after combined administration likewise lead to higher tissue concentrations, which effect might be of clinical consequence.

Adult↗
PubMed1977

[Cephradine in open heart serugery. Concentrations of cephradine in pericardial exudate and serum after cardiac surgery (author's transl)].

The diffusion of cephradine into the pericardial exudate was measured in 19 patients undergoing heart surgery. Every patient received 2 grams of cephradine (=30 mg/kg) during a 20 minute infusion period, before, during, and after the operation, and than at intervals of 6 hours, together 8 grams per day. The highest mean serum level, 10 minutes after the end of the first injection was 150 microgram/ml and after repeated dosages 102.5 microgram/ml, and at the end of the infusion interval 3.2 microgram/ml in the mean. At the time of opening the pericardium the concentration of the substance in the pericardial exudate, 15, 30, 45 and 60 minutes after finishing the first injection, varied from 4.1 to 38.6 microgram/ml in the average. The postoperative maximum levels were higher than 50 microgram/ml and 6 hours after the respective administrations the levels varied between 12.83 and 15.77 microgram/ml in each case. These high concentrations of cephradine in the pericardial exudate could not be attributed to seepage of blood into the pericardium.-At specific "check points" bacteriological materials were taken. Out of 1168 specimens only 6.7% were bacteriological positive. Drains in wounds, the pleura or the pericardium were sterile in most cases. Intravenous catheters were contaminated only in 1%. However the contamination rate rises with period of storage. The results show that cephradine exhibits good diffusion into the exudate of pericardium. With the chosen dosage the concentrations of the antibiotic in the pericardial exudate are higher than the MIC values of most pathogen bacteria.

Bacterial Infections↗
PubMed1977

[Results and experiences with automatic data processing of caries epidemiological data].

The authors report of further results from and experience with the automated processing of caries-epidemiological data by means of the R 300 computer. The extension of result documentation to qualitative characteristics of caries incidence in the framework of scientific problems is described and critically evaluated, taking a caries-protective collective action for an example.

DMF Index↗
PubMed1974

[Caries prevention by controlled mouth rinsing. Results following a 4-year control period].

The authors report on clinical results, with regard to the inhibition of the increase in caries in the permanent juvenile denture amounting to about 40%. This refers to an experimentally extablished double rinsing procedure. Because of the favourable state of affairs between caries protection, expenses, and management, the authors recommend the utilization of this procedure for group prophylaxis.

Child↗