[Basic study on isolated culture of the somatic cell and its subculture].
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Albumins↗
Biomedical subjects
I Yamane
Publications and source records attributed to I Yamane.
Long-term culture of the ascites form of a mouse mammary tumor in serum-free media.
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Albumins↗
Malignant transformation and chromosome change of two cell lines from Syrian hamster embryonic tissue in vitro.
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Animals↗
Establishment of a cell line in vitro from the lesion of a clinical case of penis cancroid.
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Animals↗
In vitro isolation and characterisation of a bovine Neospora species in Japan.
Eleven aborted bovine fetuses and five calves suspected as having neosporosis were necropsied and tissues from these animals were inoculated into bovine cardiopulmonary aortic endothelial cells and monkey kidney cells and maintained at 37 degrees C with 5 per cent CO2. Neospora tachyzoites were observed in one cell 49 days after inoculation. The isolated parasite (JPA1) was morphologically identical to the previously reported bovine Neospora species (BPA1) and confirmed by its strong antigenic reactivity with bovine control antisera to Neospora species and its lack of reactivity with Toxoplasma gondii and Sarcocystis cruzi antisera. This is the first bovine Neospora species isolate in Asia and further studies with this isolate are now expected.
Abortion, Veterinary↗