Invasive group B streptococcal disease in nonpregnant adults.
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Adult↗
Biomedical subjects
I de Miguel
Publications and source records attributed to I de Miguel.
[Keratitis caused by Acanthamoeba in patients with contact lenses].
BACKGROUND: Keratitis by Acanthamoeba is a severe infectious complication which may be derived from the use and bad preservation of contact lens. This disease la increasingly more frequent and rapid diagnosis and treatment condition the posterior evolution of the disease. PATIENTS AND METHODS: The cases of 2 contact lens waters who developed keratitis by Acanthamoeba are presented. The diagnostic methods and treatment are commented upon. RESULTS: Keratitis by Acanthamoeba was diagnosed in 2 patients following analysis of corneal scrapings and of the saline solution used for lens maintenance. Trophozoites and cystes of this parasite were observed in all the samples processed. The evolution was good in the patient treated at 15 days after initiation of the symptoms. However, the evolution was very bad in the patient who delayed in initiating the amebicide treatment. Penetrating keratoplasty was performed in both patients. CONCLUSIONS: Specific treatment with derivates of propamidine implemented early may avoid severe ocular complications. As prophylaxis for contact lens users, it is recommended that the contact lens be maintained clean and correctly care for, and the use of home-made saline solutions which are easily contaminated by Acanthamoeba, should be avoided.
Acanthamoeba Keratitis↗
[Sinusitis produced by fly larva].
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Adult↗
Proton production and consumption pathways in yeast metabolism. A chemostat culture analysis.
In this investigation, a method for the accurate quantitative determination of net proton production or consumption in biological cultures has been devised. Cells are cultured under constant pH conditions. The specific rate of proton production or consumption by the culture (qH+, mmol h-1 per g biomass) is proportional to the mmol of base or acid required to maintain constant pH per unit time, and this equivalence is independent of the buffering capacity of the culture medium. The above method has been applied to chemostat cultures of Candida utilis growing on glucose or glycerol as carbon source, and different nitrogen sources. The results indicate that the nitrogen assimilation pathway alone determines the value of qH+, and a fixed stoichiometric relationship between nitrogen uptake rate qN (meq h-1 per g biomass) and qH+ has been found for each nitrogen source employed. Thus, qH+/qN values of +1, 0 and -1 were found for ammonium ions, urea and nitrate respectively. Under oxidative metabolism, the contribution of carbon catabolism to the value of qH+ was undetectable. Sine qN may be related to growth and production of type 1 compounds in fermentation processes, the parameter qH+ was incorporated into a model of growth and energy metabolism in chemostat culture (Castrillo and Ugalde, Yeast 10, 185 - 197, 1994), resulting in adequate simulations of experimentally observed culture performance. Thus, it is suggested that qH+ may be employed as a simple and effective control parameter for biotechnological processes involving biomass-related products.
Candida↗
[Importance of anaerobic bacteria in recurrent childhood non-streptococcal tonsillitis].
BACKGROUND: The presence of anaerobes in the oropharyngeal flora was already known, but they were infrequently involved in infections, except for specific diseases (abscesses, Vincent's angina). The involvement of anaerobes in more frequent by observed oropharyngeal diseases has been recently proposed. METHODS: We analyzed the tonsillar flora of 22 healthy children (control group) and 54 children diagnosed of recurrent tonsillitis. We compare the clinical response of children with recurrent tonsillitis to two treatments: penicillin, and penicillin plus metronidazole. RESULTS: We isolated Streptococcus pyogenes from 22.2% of children with tonsillitis, and did not isolate it from any healthy children. The rest of tonsillar flora was qualitatively similar in both groups. Both populations showed a high proportion of beta-lactamase-producer bacteria. In children from whom S. pyogenes was isolated, the response to the treatment was similar for both treatments. In those cases where S. pyogenes was not isolated, penicillin + metronidazole cured the 77.2% of children treated, but penicillin cured only 30% of cases. CONCLUSIONS: These results suggest that anaerobes might be involved, at least, in recurrent tonsillitis when S. pyogenes is not found, and justify further studies about the etiology and treatment of this disease.
Bacteria, Anaerobic↗
[Use of topical ciprofloxacin in chronic suppurating otitis media].
OBJECTIVE: A prospective study is presented. We carry out an analysis of the results obtained after treatment with different protocols of administration of ciprofloxacin, during the active phase in chronic otitis media and in chronic otorrhea. MATERIAL AND METHOD: A multicenter, prospective study is carried out, in 3 ENT departments, corresponding to 3 reference tertiary hospitals. 300 patients were included ranging from 5 to 73 years old, all were diagnosed of chronic disease of the middle ear: simple chronic otitis media (n = 128), chronic otitis with bone reabsorption (n = 57), cholesteatoma infection (n = 42) and postsurgery ear infection (n = 73). Patients were placed in 5 treatment groups: ciprofloxacin (oral administration) (only adults were included), topical ciprofloxacin (0.5%), topical ciprofloxacin (0.2%), topical ciprofloxacin (0.2%) plus oral ciprofloxacin and ciprofloxacin (0.3%) plus topical fluocinolone. There was a control group treated with polimixin plus neomicine and hidrocortison. RESULTS: The most common isolated bacterias were: Pseudomonas aeruginosa and S. aureus. We found 19 resistant strains to ciprofloxacin. A better therapeutic response was observed in the topical administration groups. In topical administration, a difference was only observed in the cholesteatoma and chronic middle ear infection with bone reabsorption groups, in those patients that were administered the ciprofloxacin with fluocinolone. CONCLUSION: Forms of topical treatment, with ciprofloxacin, in active infection of chronic disease of the middle ear, improve the results compared to oral administration. The association with of topical fluocinolone improves the results in the cases with cholesteatoma infection and chronic middle ear infection.
Administration, Topical↗
[Corynebacterium amycolatum: sepsis in hematologic patients].
BACKGROUND: To evaluate clinical and microbiological characteristics of sepsis due to Corynebacterium amycolatum, because few cases have been reported in human infections by this bacteria. METHODS: We report two cases of sepsis due to Corynebacterium amycolatum, the patients were diagnosed as having leukaemia. The identification procedures are discussed. RESULTS: We describe 2 patients with sepsis in neutropenic phase. Antibiotic treatment was successful in both cases. Clinical isolates were classified as Corynebacterium xerosis by the Api Coryne system Version 1.0. The chemotaxonomic characteristics of the isolates were determined by thin-layer chromatographic analysis. Isolates did not contain mycolic acids, they were identified as Corynebacterium amycolatum. CONCLUSIONS: Sepsis due to Corynebacterium amycolatum is a rare disorder. A test for mycolic acid could be sufficient to make a distinction between Corynebacterium amycolatum and other diphteroid bacteria. Such a test involves thin-layer chromatography.
Aged↗