Biomedical subjects

Ia Bŭchvarova

Publications and source records attributed to Ia Bŭchvarova.

5 recordsLinked to original sources

PubMed1987

[Experiments to identify Mycoplasma isolates from cell cultures].

Identified were various species of Mycoplasma organisms isolated from three continuous cell lines--BHK-21, SPZF, and TT. On the base of cultural, biochemical, and serological investigations the isolates were defined as M. bovirhinis, M. arginini, and A. laidlawii. The bovine sera, used to culture the cell lines were shown to be the basic source of contamination with Mycoplasma organisms. This made it necessary to carry out a preliminary study on each batch of serum for Mycoplasma contamination prior to its use for laboratory and productional needs.

Animals↗

PubMed1986

[A method for demonstrating mycoplasmas].

Bisbenzimid-marked continuous cell lines were investigated via fluorescent microscopy. The presence of Mycoplasma DNA was demonstrated in the cytoplasm with some of them. The method employed for the demonstration of Mycoplasma contamination is readily applicable, and could be used for control on continuous cell lines.

Animals↗

PubMed1985

[Isolation of Campylobacter spp. from calves in Bulgaria].

Studied were a total of 58 samples, containing intestinal content of calves that had died of enteritis as well as fecal samples and samples from clinically normal calves. Campylobacter spp. were isolated in 8.8 per cent of the cases. The isolated 5 strains was determined as Campylobacter fetus subsp. intestinalis. This is the first communication on the isolation of Campylobacter spp. in this country.

Animals↗

PubMed1985

[Isolation of Haemophilus somnus from cattle].

Studied were a total of 220 samples of material taken from the respiratory tract of calves and from fetuses of cows that miscarried for the presence of Haemophilus bacteria. Haemophilus somnus was isolated for the first time in this country from the respiratory tract of calves (10.1 per cent) and from fetuses of cows with abortions (6.2 per cent). Haemophilus somnus was found in calves that had contracted respiratory diseases in 13 per cent of the cases, and in clinically normal calves--in 3.5 per cent of the cases. It was isolated both in pure culture and in mixed culture with P. multocida, M. bovirhinis, M. arginini, V. fetus, and C. pyogenes. As many as 21 strains of Haemophilus somnus were isolated, having the same morphologic, cultural, and biochemical properties characteristic of the species.

Abortion, Veterinary↗

PubMed1982

[Abortions in cows caused by Corynebacterium pyogenes].

Bacteriologic investigations of embryos, fetuses, and placentae of cows that had miscarried, uterine and milk samples taken from animals with endometritis and mastitis, and bull semen samples revealed the presence of Corynebacterium pyogenes. It was established that this organism ranks first in the group of occasionally pathogenic microflora that was associated with the occurrence of abortions in cows. It was isolated from in 9.2 per cent of the cases from fetuses, 5.3 per cent of the cases from embryos, and 8.1 per cent of the cases from placentae. On farms with records of Corynebacterium pyogenes abortions all endometritis and mastitis cases could be associated with the same infection. The organisms was demonstrated in the uterine secretion in both clinically normal cows and bull semen. Corynebacterium pyogenes was found to be viable for a long time, and, therefore, represented a serious source of infection--abortions, endometritis, and mastitis.

Abortion, Septic↗