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Ia G Kishko

Publications and source records attributed to Ia G Kishko.

At least 73 records · Page 4Linked to original sources
PubMedDate not supplied

[Physicochemical and biological properties of the DNA of virulent and temperate Erwinia carotovora phages].

DNA of virulent and moderate E. carotovora viruses, of marked AT-type, was shown to have molecular weights determine by 2 independent methods from 49.2 to 54 and 28 to 29.8 MD, respectively. The genomes of the viruses under study differed by the point and curve profiles of the melting temperature and sedimentation constant. DNA of the moderate phage contains an unidentified abnormal base and has no free cohesive ends. DNA of the moderate virus contains 15% of nucleotide sequences homologous with those of the virulent virus DNA. Calcium-competent cells are the most acceptable system for the study of DNA transfection of these phages. The virulent virus DNA gives a higher yield of transfectants (by 2 orders) than that of the moderate virus. Herring DNA reduces the level of DNA transfection of both phages approximately by 100-fold. In competitive transfection experiments, the virulent virus DNA was found to be more competitive.

Bacteriophages↗
PubMedDate not supplied

[Electron microscopic structure of Erwinia carotovora moderate phage 59 and its DNA].

Electron microscopic examinations showed the moderate phage 59 Erwinia carotovora to consist of a head of icosahedral shape 50.0+/- +/-2.5 nm in diameter, a noncontractile process 150.0+/-3.5 nm in length and 8.2+/-1.0 nm in diameter with a basal plate of 20.0+/-1,5 nm. The head of the virion of 45.9 X 10(3) nm3 in volume is formed by 812 morphological subunits about 3.0 nm in diameter; the number of structural subunits is 4860 on the basis of triangulation number (81). The process of the virus is cross-striated, the pattern being formed by 35+/-1 rows (43+/-5 A) of subunits 3.0 nm in diameter. The total number of subunits in the process is 210+/-6. DNA of the virus under study has a trend of ring formation. Its modal length is 14.61+/-0.53 micron corresponding to a molecular weight of 28.66+/-1.04 X 10(6) daltons. The volume of DNA molecule is 45.9 X X 10(3) nm3, indicating that a portion of phage DNA is in the virion, probably in denaturated state. An electron microscopic model of the organization of the virus under study is proposed.

Bacteriophages↗
PubMedDate not supplied

[The effect of hydrodynamic conditions on the interferon-synthesizing activity of swine splenocytes in the production of alpha-interferon].

Parameters of physical constituents of the agitation process and mass-exchange characteristics of the suggested bioreactor for suspension cultivation of pig splenocytes are determined. The effect of hydrodynamic conditions on viability and interferon-synthesizing activity of cells in production of alpha-interferon of pigs has been studied. The relation existing between interferonogenesis processes proceeding under suspension and under stationary biosynthesis has been analyzed by the pair correlation method.

Animals↗
PubMedDate not supplied

[The isolation and purification of porcine interferon-gamma].

Porcine immune interferon (IF-gamma) was generated by stimulation with bacterial lectins. Physicochemical analysis showed porcine IF-gamma to be a glycoprotein with an isoelectric point around 8.4-8.8 and an apparent molecular weight of 28000 to 30000. A purification process used in this work included sequential steps of precipitation with ammonium sulphate and chromatographic separation on GPG-glass, con A-Sepharose and biogel P-100. This purification process gave a preparation of IF-gamma with specific activity of around 7 x 10(4) units per mg of protein.

Animals↗