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Ichiko Akatsuka

Publications and source records attributed to Ichiko Akatsuka.

2 recordsLinked to original sources

[Case of anterior ischemic optic neuropathy accompanied by Churg-Strauss syndrome].

BACKGROUND: Churg-Strauss syndrome (CSS) is a vasculitis which includes the three signs of bronchial asthma, hypereosinophilia, and vasculitis syndrome. We report a case in whom anterior ischemic optic neuropathy (AION) accompanied CSS. Such a case has not been reported previously in Japan. CASE: A 66-year-old woman with a history of bronchial asthma and eosinophilic pneumonia was hospitalized for fever, unusual feelings in both plantae and in a finger of her left hand, disturbance of visual acuity and visual field in the right eye, and skin rashes; a diagnosis of CSS was made. At the time of the first ophthalmological examination, AION was observed. Steroid therapy was started, but the treatment was not effective, despite an improvement in choroidal circulation confirmed by fluorescein angiography and indocyanine green angiography. In the unaffected eye, soft exudates appeared when the steroid dose was lowered and disappeared when the steroid dose was raised. CONCLUSION: Anterior ischemic optic neuropathy accompanied with Churg-Strauss syndrome resisted medical treatment and the prognosis was poor.

Aged↗

lambda-crystallin related to dehydroascorbate reductase in the rabbit lens.

PURPOSE: To evaluate the relationship of lambda-crystallin to reduced nicotinamide adenine dinucleotide (NADH)-dependent dehydroascorbate (DHA) reductase found specifically in the rabbit lens. METHODS: DHA reductase Fractions I-IV were separated from the lambda/betaL1-crystallin fraction of rabbit lens soluble protein by diethylaminoethyl (DEAE)-cellulose ion-exchange column chromatography, and then the enzyme was partially purified from Fraction II by rechromatography on the same ion-exchange column. The isolated DHA reductase fractions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting, native isoelectric focusing and two-dimensional gel electrophoresis. RESULTS: Using Western blot and a probe of antiserum to recombinant lambda-crystallin, the main 33-kDa protein band was strongly stained in all the rabbit lens DHA reductase fractions, and most of the additional protein bands of approximately 25-30 kDa were also detectable. In the partially purified enzyme, the 33-kDa subunit alone was identified as a distinct protein band by SDS-PAGE, and a main basic protein was found at pI 7.6 by native isoelectric focusing. In addition, many bands of more acidic proteins were separated from other enzyme fractions, and protein spots corresponding to the 33 and/or approximately 25-30-kDa subunits were detected in each of the more acidic proteins by two-dimensional gel electrophoresis. CONCLUSION: These results suggest that lambda-crystallin is closely related to the DHA reductase in the rabbit lens. The above heterogeneity of the enzyme-crystallin may arise from posttranslational modifications.

Amino Acid Sequence↗