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Iftikhar Qayum

Publications and source records attributed to Iftikhar Qayum.

6 recordsLinked to original sources
PubMedDate not supplied

Presentation of pulmonary tuberculosis at Ayub Teaching Hospital Abbottabad.

BACKGROUND: Pulmonary tuberculosis (PTB) may be easily confused with other chest diseases during its initial presentation. This study was carried out to identify presenting clinical and laboratory features that differentiate PTB from other diseases and to correlate clinical features and laboratory findings. METHODS: This study was carried out at the Department of Pulmonology, Ayub Teaching Hospital Abbottabad, from September 1999 to December 2000. A total of 46 patients were included in the study after being clinically diagnosed as pulmonary tuberculosis. These patients were subjected to detailed history taking recording age, sex, weight, socioeconomic status and smoking habits. They were clinically evaluated and laboratory tests including Hemoglobin, ESR, TLC, DLC and sputum for AFB were done. They were put on standard antituberculous therapy and followed from 2 to 5 months to monitor treatment effect. Statistical analysis was performed by SPSS 8 computer program. RESULTS: A bimodal presentation (below age 30 years and above age 50 years), fever, productive cough, weight loss, night sweats and raised ESR were the most common findings in PTB patients. Sputum AFB smears were positive in 50% of diagnosed cases. No correlation was found between clinical and laboratory parameters in establishing a confident diagnosis of the disease. CONCLUSIONS: The study highlights the importance of further research to pinpoint stronger and more reliable criteria for diagnosis.

Adolescent↗
PubMedDate not supplied

Increased DNA methyltransferase 1 (DNMT1) gene expression in human lymphomas by fluorescent in situ hybridization.

BACKGROUND: The DNA Methyltransferase 1 (DNMTI) gene is among the better known 'epigenetic' systems that can regulate normal and abnormal gene expression as well as create 'hot spots' for DNA mutations. Its role has been studied in a number of malignancies with important implications for involvement in early events of malignant transformation. The present study describes the findings with respect to expression of this gene in human lymphomas studied by Fluorescent In Situ Hybridization (FISH). METHOD: The study was undertaken on randomly selected archival human lymph nodes comprising 50 specimens of normal or reactive lymph nodes and 50 specimens of lymphoma lymph nodes. These were subjected to FISH using oligonucleotide Antisense probes for the DNMT1 mRNA according to standard FISH protocols. Percent cells stained, mean 'dots' stained per cell and staining signal intensity were taken as the criteria for comparing control and lymphoma lymph nodes. Quantitation of probe signals was done both by manual visualisation of the fluorescent signals and computer based image analysis. RESULTS: Data indicated a significantly increased expression of the DNMT1 mRNA in lymphoma cases as compared to controls (p<0.001). CONCLUSION: This implies a possible role of the DNMT1 gene in transformation / oncogenesis in human lymphomas.

Base Sequence↗
PubMedDate not supplied

Partial hydatidiform mole along with term gestation and alive baby.

Gestational trophoblastic disease consists of a broad spectrum of conditions ranging from an uncomplicated partial hydatidiform molar pregnancy to stage-IV choriocarinoma with cerebral metastases. We describe a partial molar change in the placenta that was associated with a normal female fetus that was delivered at term and is alive and healthy.

Adult↗
PubMedDate not supplied

Release of vasopressin during suppression of oestrous cycle in rat by zoladex and hypovolemic challenge.

BACKGROUND: The vasopressin (AVP) response to hypovolemia has been compared in intact and chemically castrated rats. This functional ovariectomy was done to confirm the findings in surgical ovariectomy of how gonadal steroids modulate the release of AVP under hypo-volemic challenge. METHODS: Twenty female Sprague Dawley rats were checked for oestrous over two consecutive cycles. The ten control rats were given sub-cutaneous puncture only whereas the experimental were given Zoladex implant. On the fifteenth day all the rats were given intra-peitoneal injection of poly-ehylene glycol. All the rats were de-capitated after an hour. RESULTS: The uterine weight was significantly decreased in experimental group. The plasma AVP level was also significantly decreased in the experimental group. The pituitary AVP level was significantly increased in the experimental group. CONCLUSION: The chemical castration effected the AVP secretion, this proves that the sex steroids modulate the release of AVP secretion inspite of hypo-volemic challenge.

Animals↗
PubMedDate not supplied

Dna methyltransferase 1 (DNMT1) gene activity in human lymphomas correlates with aberrant p53 gene expression.

BACKGROUND: The DNA Methyltransferase 1 (DNMT1) gene has been implicated as a mutagen for tumor suppressor genes by causing hypermethylation and subsequent TA mutations of CpG islands located in the promoter regions of these genes. The present study was undertaken to determine if increased DNMT1 gene activity correlated with increased aberrant p53 gene expression in human lymphomas. METHODS: The study was undertaken on randomly selected archival human lymph nodes comprising 50 normal or reactive lymph nodes and 50 lymphoma lymph nodes. These were subjected to Fluorescent In Situ Hybridization (FISH) using oligonucleotide Antisense probes for the DNMT1 and the p53 mRNA according to standard FISH protocols. Percent cells stained, mean 'dots' stained per cell and staining signal intensity were taken as the criteria for comparing control and lymphoma lymph nodes. Quantitation of probe signals was done both by manual visualization of fluorescent signals and computer based image analysis. Correlation analysis was performed by calculation of Pearson's correlation coefficient. RESULTS: Data indicated significantly increased expression of the DNMT1 and the p53 mRNA in lymphoma cases as compared to controls (p < 0.001). Moreover significant correlation was obtained for the expressions of these two genes in lymphomas (p < 0.001), but not in control lymph nodes. CONCLUSION: Increased DNMT1 gene activity may contribute to increased p53 gene expression in human lymphomas, supporting a mutagenic role for the DNMT1 gene.

Case-Control Studies↗