Biomedical subjects

Igor V Filippov

Publications and source records attributed to Igor V Filippov.

5 recordsLinked to original sources

PubMed2006

Sound-induced changes of infraslow brain potential fluctuations in the medial geniculate nucleus and primary auditory cortex in anaesthetized rats.

Recent publications indicate the presence of infraslow activity (<0.5 Hz) in subcortical and cortical sites of the auditory system of the brain. It has been reported that this activity might be sensitive to acoustic stimuli. Yet the dynamics of infraslow brain potential (ISBP) fluctuations in these structures and their potential sensitivity to auditory stimuli are unknown. The present study was performed in order to test the hypothesis that extracellular ISBP activity in the medial geniculate nucleus (MGN) and the primary auditory cortex (A1) responds concurrently to acoustic stimuli. The experimental subjects were 5 adult rats with chronic stereotaxic electrodes implanted in MGN and A1. The animals were anesthetized and recordings were made in both sites during both silence and rhythmical acoustic stimulation. Our results support the hypothesis that these fluctuations are sensitive to acoustic stimuli. There were similar changes in ISBP activity in the MGN and A1 in response to rhythmic acoustic stimulation. Specifically, there were significant increases in the frequency range of seconds. Based on these findings, we suggest that sound-correlated changes in infraslow activity in the range of seconds in the MGN and A1 reflect specific mechanisms of neural processing of acoustic information in the auditory system of the brain.

Acoustic Stimulation↗

PubMed2005

Very slow brain potential fluctuations (< 0.5 Hz) in visual thalamus and striate cortex after their successive electrical stimulation in lightly anesthetized rats.

Recently accumulating evidence demonstrates the presence of very slow activity (< 0.5 Hz) in structures of the visual system of the brain. It was found in our laboratory earlier that specific and significant alterations of this activity (mainly in the domain of seconds) occurred in the visual system in response to illumination changes. The present study was performed in order to test the hypothesis that potentials in the domain of seconds reflect specific and direct interactions of the lateral geniculate nucleus (LGN) and the primary visual cortex (V1) during neural processing of sensory information. The experimental subjects were seven adult rats with chronic stereotaxic electrodes implanted in the LGN and V1. Animals were lightly anesthetized and the recordings were made in the LGN before and after electrical stimulation of V1 and also in the V1 before and after stimulation of LGN. The main findings were significant spectral changes in the domain of seconds in the V1 after LGN electrical stimulation and similar changes in the LGN after V1 electrical stimulation. These changes were manifested as significant increases in power in the domain of seconds (0.1-0.5 Hz). Significant responses were detected in the both LGN and V1 multisecond activities (pre- vs. post-stimulus recordings). The changes were opposite in direction in the LGN and V1. The obtained results support the conclusion that very slow activity in the domain of seconds reflects specific mechanisms of forward and backward interactions within the LGN-V1 thalamic-cortical-thalamic system, while multisecond activity relates to global neuronal activity fluctuations.

Anesthesia↗

PubMed2005

Very slow potentials in the lateral geniculate complex and primary visual cortex during different illumination changes in freely moving rats.

Previous literature has shown different forms of very slow oscillatory phenomena (0-0.5 Hz) in the structures of brain visual system. It was demonstrated in aforementioned publications that this infraslow activity might be sensitive to the level of environmental illumination. This study was performed to test the hypothesis that extracellular very slow brain potential (VSBP) oscillations or fluctuations in lateral geniculate complex (LGC) and primary visual cortex (PVC) are responding specifically and concurrently to different illumination changes. Experiments were conducted on five albino rats with chronically implanted stereotaxic electrodes in LGC and PVC brain sites. Our results support the aforementioned suggestion and revealed significant and similar patterns of VSBP modification within a frequency domain of seconds (0.1-0.25 Hz) in both the LGC and PVC in response to darkness, illumination and photostimulation. It is also documented here that significant and similar changes of multisecond activity (0.02-0.04 Hz) occur in both investigated brain sites in response to photostimulation only. Based on these findings, we propose that a possible role for VSBP oscillations in the LGC and PVC should be strongly considered in the CNS mechanisms of visual information processing.

Animals↗

PubMed2004

Very slow potential oscillations in locus coeruleus and dorsal raphe nucleus under different illumination in freely moving rats.

Recent findings have revealed very slow (<0.5 Hz) oscillatory phenomena in the structures of the brain visual system. It has been proposed that very slow brain potentials in an extremely slow domain, less than 0.1 Hz, recorded from the lateral geniculate complex and primary visual cortex are associated with periodic influences originating from the locus coeruleus and dorsal raphe nucleus. The present study was performed to test the hypothesis that extremely slow brain potential oscillatory patterns in the locus coeruleus and dorsal raphe nucleus during several types of visual stimulation--light exposure, darkness, and photostimulation--are similar to those in the primary visual cortex and lateral geniculate complex under the same conditions of illumination. The results support this hypothesis. Specifically, spectral patterns of multisecond oscillations in the range of 0.02-0.04 Hz and fluctuations in the domain of minutes (below 0.002 Hz) were present in both the locus coeruleus and dorsal raphe nucleus and were similar to those found in the primary visual cortex and lateral geniculate complex. Additionally, we detected significant increases in the power spectra of multisecond oscillations in both nuclei in response to photostimulation (P<0.05). Our tentative conclusion is that extremely slow potentials in the locus coeruleus and dorsal raphe nucleus contribute to the regulation of extremely slow activity in the brain visual system.

Action Potentials↗

PubMedDate not supplied

PROSIT: pseudo-rotational online service and interactive tool, applied to a conformational survey of nucleosides and nucleotides.

A Pseudo-Rotational Online Service and Interactive Tool (PROSIT) designed to perform complete pseudorotational analysis of nucleosides and nucleotides is described. This service is freely available at http://cactus.nci.nih.gov/prosit/. Files containing nucleosides/nucleotides or DNA/RNA segments, isolated or bound to other molecules (e.g., a protein) can be uploaded to be processed by PROSIT. The service outputs the pseudorotational phase angle P, puckering amplitude numax, and other related information for each nucleoside/nucleotide detected. The service was implemented using the chemoinformatics toolkit CACTVS. PROSIT was used for a survey of nucleosides contained in the Cambridge Structural Database and nucleotides in high-resolution crystal structures from the Nucleic Acid Database. Special cases discussed include nucleosides having constrained sugar moieties with extreme puckering amplitudes, and several specific DNA/RNA helices and protein-bound DNA oligonucleotides (Dickerson-Drew dodecamer, RNA/DNA hybrid viral polypurine tract, Z-DNA enantiomers, B-DNA containing (L)-alpha-threofuranosyl nucleotides, TATA-box binding protein/TATA-box complex, and DNA (cytosine C5)-methyltransferase complexed with an oligodeoxyribonucleotide containing transition state analogue 5,6-dihydro-5-azacytosine). When the puckering amplitude decreases to a small value, the sugar becomes increasingly planar, thus reducing the significance of the phase angle P. We introduce the term "central conformation" to describe this part of the pseudorotational hyperspace in contrast to the conventional north and south conformations.

Nucleic Acid Conformation↗