Biomedical subjects

Irfan Kaygusuz

Publications and source records attributed to Irfan Kaygusuz.

At least 19 recordsLinked to original sources

PubMed2006

[Ear symptoms accompanying temporomandibular joint diseases].

OBJECTIVES: We investigated symptoms of otologic diseases in patients with temporomandibular joint diseases. PATIENTS AND METHODS: The study included 44 patients (26 females, 18 males; mean age 36+/-10.7 years; range 21 to 58 years) with temporomandibular joint disease. A questionnaire was administered to the patients to inquire into otological symptoms accompanying temporomandibular joint disease. RESULTS: Temporomandibular joint disease was bilateral in 11 patients. Accompanying otological symptoms were as follows: click voice with jaw movements (n=21; 47.7%), headache (n=15; 34.1%), pain in the ear with jaw movements (n=14; 31.8%), tinnitus (n=8; 18.2%), fullness in the ear (n=6; 13.6%), vertigo or dizziness (n=6; 13.6%), and hearing loss (n=3; 6.8%). Pure-tone audiometry revealed sensorineural hearing loss in one patient, being 28 dB on the right and 30 dB on the left. CONCLUSION: Our findings suggest that otologic symptoms may accompany temporomandibular joint diseases, requiring that patients presenting with otologic symptoms must be investigated for temporomandibular joint diseases.

Adult↗

PubMed2005

Bacteriemia during mastoidectomy and/or tympanoplasty.

OBJECTIVE: To investigate the risk of bacteriemia development during surgery in patients who underwent mastoidectomy and/or tympanoplasty for chronic otitis media. PATIENTS AND METHODS: A total of 59 patients with chronic otitis media who were undergoing tympanoplasty operation with or without mastoidectomy were enrolled in this study. Smear cultures were obtained from outer ear canal and/or middle ears of all patients before the operation. Venous blood samples were obtained before and after the operation for bacteriologic analysis. Smear cultures were also obtained from the pressure dressing material that was applied during the operation for retrieval of the outer ear canal pressure. RESULTS: There was a 13.5% difference between the outer ear canal and/or middle ear smear cultures and ear pressure dressing smear cultures of the same case. There was no growth in the blood cultures obtained before tympanoplasty in any of the involved cases, however, in 5 (8.4%) of the culture samples obtained immediately after the tympanoplasty operation, there was bacteriemia. In the pressure dressing smear cultures obtained after the operation, 11 patients had microbial growth. CONCLUSION: Risk of bacteriemia should be considered in the preoperative period for the patients undergoing mastoidectomy especially in patients with cardiovascular diseases, this is of importance for the dramatic consequences that might arise after the operation. We also think that bacteriemia is also one of the factors that influences graft success rate.

Adolescent↗

PubMed2005

Detection of Helicobacter pylori in children with otitis media with effusion: a preliminary report.

OBJECTIVE: To determine the presence of Helicobacter pylori in the middle ear effusion of patients with otitis media with effusion (OME) by polymerase chain reaction (PCR). STUDY DESIGN: A prospective study in patients with OME. METHODS: The study was performed in 38 patients with OME who were admitted to the ENT Clinic, Firat University from June 2003 to April 2004. In all cases, a myringotomy operation (with or without placement of a ventilation tube) was carried out. The effusion samples aspirated from the middle ear were analyzed with PCR assay. RESULTS: A total of 55 aspiration samples collected from 38 children ranging in age from 2 to 12 were included in the study. Fifteen of the subjects were girls, and 23 were boys. In 17 patients, both ears demonstrated effusions, whereas in 21 patients, only one ear had effusions. Nine (16.3%) of 55 the middle ear effusion samples were shown to be H. pylori positive by PCR. CONCLUSIONS: H. pylori was detected in the middle ear effusion of some patients with OME. These results may have interesting implications for a possible role of H. pylori in OME. In addition, these results suggest that further studies are needed to investigate the role of H. pylori in the etiology of OME.

Child↗

PubMed2005

Roles of free radicals, nitric oxide, and scavenging enzymes in nasal polyp development.

The aim of this study was to investigate the role of nitric oxide (NO), free oxygen radicals, and scavenging enzymes in the development of nasal polyp (NP) disease. This study included 41 patients who underwent endoscopic surgery because of NPs. Control specimens were taken from the inferior turbinate of 32 patients who underwent septoplasty. The levels of malondialdehyde (MDA), NO, and superoxide dismutase (SOD) were measured in intraoperative specimens of polyp tissue and turbinate mucosa. The levels of tissue NO were 191.06 +/- 26.62 micromol/mg of protein in patients with NPs and 145.30 +/- 19.19 micromol/mg of protein (p < .001) in the control group. The levels of MDA in the study and control groups were 12.47 +/- 2.12 nmol/mg and 8.83 +/- 1.08 nmol/mg (p < .01), respectively. The levels of SOD in the study and control groups were 50.77 +/- 14.74 U/mg and 77.93 +/- 15.31 U/mg (p < .001), respectively. It was determined that the levels of MDA in plasma and erythrocytes were higher in the patients with NPs than in the control group (p < .05). The levels of NO in plasma and erythrocytes in both groups were similar. The levels of SOD in plasma and erythrocytes were lower in patients with NPs than in the control group (p > .05). Increases in the levels of tissue MDA and NO and decreases in scavenging enzymes in patients with NPs as compared to control groups may indicate the presence of free radical damage in patients with nasal NPs. New studies are needed to clarify the efficacy of using antioxidants in the treatment of NPs.

Adult↗

PubMed2005

Identification of Chlamydia trachomatis with polymerase chain reaction in middle ear fluid in otitis media with effusion.

In this study, 78 middle ear aspiration samples from 59 children between the ages of 2 14 who have undergone myringotomy under general anesthesia were included. The presence of DNA genome of Chlamydia trachomatis was analyzed with polymerase chain reaction in the aspiration material. The relationship between the nature of the effusion fluid and the presence of Chlamydia trachomatis was investigated. Of the seventy eight middle ear effusions, 26 (33.3%) were serous, 31 were (39.7%) mucoid and 21 (26.9%) were sero mucoid in nature. Chlamydia trachomatis DNA genome was identified in seven (8.9%) effusion samples. Of these, three were mucoid (42.8%), three were serous (42.8%) and one was sero mucoid (14.2%) in character. We think that during the bacteriological analyses carried out for the cases of otitis media with effusion, Chlamydia trachomatis should be taken into consideration both at the time of diagnosis and the treatment.

Adolescent↗

PubMed2004

Hearing in workers exposed to low-dose radiation for a long period.

The aim of the present study was to evaluate changes in hearing thresholds with standard and high frequency audiometry in workers exposed to low-dose ionizing radiation for a long period. A total of 57 (49 male and 8 female) technical staff working in radiology-related jobs who were exposed to occupational radiation were included in the study. The control group consisted of 32 (27 male and 5 female) volunteer subjects with normal hearing. The symptoms like tinnitus, vertigo, weakness and lack of appetite were evaluated. A standard ascending/descending method was applied to the subjects of the study and the control groups in order to determine their hearing thresholds at eleven different frequencies between 250 and 16,000 Hz. In the study group, the working duration of subjects ranged from 4 to 23 years, and the percentage of tinnitus, weakness, vertigo and lack of appetite were 47%, 28%, 24% and 17%, respectively. It was observed that pure tone hearing thresholds were markedly increased for 4,000, 6,000, 8,000, 14,000 and 16,000 Hz frequencies in the study group compared to the control group (p<0.01). Levels of static compliance and middle ear pressures of the study group were similar to the control group. Tinnitus, vertigo and hearing loss in high frequencies were observed in the subjects exposed to the radiation for a long period. Subjects under high risk should be evaluated periodically. We suggest that the use of standard and high frequency audiometry together could be beneficial in the evaluation of these subjects.

Acoustic Impedance Tests↗

PubMed2004

Prevalence of otitis media with effusion in first and second grade primary school students and its correlation with BCG vaccination.

OBJECTIVE: The object of this study was to determine the prevalence of otitis media with effusion (OME) in first and second grade primary school students, to analyze the causes of the difference in the prevalence, to define the effect of OME on the academic performance of the children and to investigate a correlation between the prevalence of OME and Bacillus Calmette-Guerin (BCG) vaccination. STUDY PLAN AND METHODS: The study was conducted during the September-November, 2002 period in Elazig. A total of 3675 clinically healthy primary school children attending primary schools in the central district of Elazig, living in the same region and of a similar socioeconomic status and age group were included in the study. Of the 3675 children, 2042 were in their first and 1633 in their second grade of primary school. The routine ear-nose and throat examinations of the children were carried out at their schools by the same medical team. The tympanometric test was performed in children diagnosed with OME following otoscopic examination. A scale measuring the academic performance of the children was developed. This scale was filled in by the student's teachers prior to physical examination. RESULTS: OME was diagnosed in 64 out of 2042 (3.1%) first grade and in 25 out of 1633 (1.5%) second grade students. The difference between the percentages of OME in first and second grade students was statistically significant (P < 0.05). The frequency of other ear-nose and throat pathologies accompanying OME was similar to those in children without OME. There was no statistically significant difference between the academic performances of children with and without OME (P > 0.05). CONCLUSION: The analysis of risk factors for OME revealed no difference between first and second grade students. The reason for the difference in the prevalence of OME between first and second grade students may be the positive effect on the immune system of the BCG vaccine which had been administered to the second grade students 4 months previously.

BCG Vaccine↗

PubMed2004

Effects of interferon-alpha2b on hearing.

The aim of the present study was to investigate the effects of interferon-alpha2b treatment on hearing in patients with chronic active hepatitis B. Twenty-six patients with chronic active hepatitis B were enrolled in the study, and pure-tone audiometry was performed to determine hearing thresholds of the patients before and at the end of 6 months of interferon-alpha2b treatment. There was no significant change in hearing thresholds of patients after treatment with interferon-alpha2b (p>0.05). The results of the present study indicate that interferon-alpha2b therapy does not have any negative effect on hearing thresholds of patients with chronic active hepatitis B. There is a need for further studies involving larger numbers of patients to allow conclusions to be drawn regarding the safety of this therapy with respect to hearing.

Adolescent↗

PubMed2004

Comparison of free radicals and antioxidant enzymes in chronic otitis media with and without tympanosclerosis.

OBJECTIVE/HYPOTHESIS: The pathogenesis of tympanosclerosis is unclear. The study was performed to investigate the role of nitric oxide, free oxygen radicals, and antioxidants in development of tympanosclerosis in patients with chronic otitis media. STUDY DESIGN: A prospective study in patients with nasal polyps. METHODS: Sixty-five patients who underwent tympanoplasty or tympanoplasty together with mastoidectomy were included in the study. Preoperative venous blood samples were drawn, and serum sodium, potassium, calcium, alkaline phosphatase, inorganic phosphorus, parathormone, and calcitonin levels were measured. The patients who had tympanosclerotic plaques on tympanic membrane or middle ear mucosa or near the ossicular chain or mastoid bone were designated as group 1 (n = 34), and the remaining patients as group 2 (n = 31). Intraoperatively, specimens were obtained from the middle ear mucosa and tympanic membrane to measure nitric oxide and malondialdehyde levels. In addition, plasma malondialdehyde levels and erythrocyte superoxide dismutase and catalase activity were determined. RESULTS: All patients had similar demographic features and serum electrolyte and hormone levels. Nitric oxide and malondialdehyde levels of the specimens obtained from the middle ear mucosa (P =.001) and tympanic membrane (P =.01) and, in parallel to this, the plasma malondialdehyde activity level were higher in group 1 than in group 2. Moreover, group 2 had significantly lower erythrocyte catalase activity levels (P =.001) compared with group 1, whereas such a significant difference was not present for superoxide dismutase activity levels (P >.05). CONCLUSION: The study results suggest that nitric oxide, free oxygen radicals, and catalase may have a role in the development of tympanosclerosis in patients with chronic otitis media.

Adult↗

PubMed2004

Meniere's disease and allergy: allergens and cytokines.

The aim of this article is to evaluate the role of allergy in the pathogenesis of Meniere's disease by means of cytokine profiles, allergic parameters and lymphocyte subgroups. A total of 46 patients aged between 26-68 years diagnosed with Meniere's disease between 1993-2002 were recruited to this study. The control group consisted of 46 healthy volunteers who were from the same age group, living in the same region and possessing similar socioeconomic indicators. Lymphocyte subgroups were measured from the peripheral blood by employing Becton Dickinson (BD) monoclonal CD4, CD8, CD23 antibodies. IFN-gamma, IL4, total IgE levels, and specific IgE levels pertaining to tree, fungus, fruit, egg-white, cow's milk, wheat flour, corn flour, beef, and rice allergens, in all seasons, were measured and compared in the patient and control groups. In patient serum samples there were positive correlations between CD23 and IgE, CD8 and IgE, CD4/CD8 and IgE, and CD23 and CD8 (p <0.01). There were negative correlations between IL-4 and IFN-gamma, IFN-gamma and IgE, and a positive correlation between IL-4 and IgE. Total IgE levels were above the normal values in 19/46 (41.3 per cent) of the patient group, but the ratio was nine out of 46 (19.5 per cent) in the control group. A history of allergy was found in 31/46 (67.3 per cent) when the patients were questioned. The ratio of a history of allergy was 16/46 (34.7 per cent) in the control group. When specific IgE levels were evaluated the ratio of patients with all the panels negative was eight out of 46 (17.9 per cent), but it was 31/46 (67.3 per cent) in the control group. This study found that the prevalence of allergy was higher in patients with Meniere's disease than in the control group. Thus the authors suggest that allergy should be taken into account when patients with this disease are treated.

Adult↗

PubMed2004

The role of allergy in the etiology of otitis media with effusion; immune system and cytokines.

OBJECTIVES: Otitis media with effusion (OME) is one of the major causes of hearing loss in childhood. In this study, the role and the importance of allergy in the etiology of OME was evaluated with reference to the immune system and the cytokines. PATIENTS AND METHODS: Seventy-eight ears of 59 patients who had undergone myringotomy with the diagnosis of OME were included in this study. Serum samples of 26 healthy children who were in the same age group and were living in the same region under similar socioeconomic circumstances were examined as the control group. RESULTS: Interferon (IFN)-gamma levels were significantly lower and IL-4 and IgE levels were significantly higher in the serum samples of the patient group when compared with those of the control group (p<0.05). A positive correlation was found between IL-4 and IgE in the serum samples of patients (p<0.01). However, there were negative correlations between IFN-gamma and both IgE and IL-4 levels, that were not statistically significant. CONCLUSION: T-helper polarization suggests that allergy may have a role in the etiology of OME. Therefore, it should be kept in mind in the treatment of OME.

Case-Control Studies↗

PubMed2003

Early stage impacts of tonsillectomy on immune functions of children.

OBJECTIVE: The purpose of this study was to investigate the changes in the humoral and cellular immunity of patients with chronic tonsillitis before and 1 month after tonsillectomy. PATIENTS AND METHODS: 37 patients scheduled for tonsillectomy were enrolled in this study. The levels of CD3+, CD4+, CD8+, CD19+, CD25+ and CD16+ + 56+ were measured for cellular immunity, and levels of IgG, IgA, IgM, C3 and C4 were measured for humoral immunity in blood samples taken from these patients before and 1 month after the operation. RESULTS: The levels of CD3+, CD8+ and CD19+ were reduced in post-operative period as compared to pre-operative period but this was not statistically significant (P > 0.05). However it was found that the level of CD4+ was significantly increased while the level of CD25+ was reduced (P < 0.05) in the post-operative period. There were statistically significant differences between pre- and post-operative levels of immunoglobulins, C3 and C4, which were decreased after tonsillectomy (P < 0.05), but these levels were comparable with those of the control group. CONCLUSION: The results from the present study indicate that humoral and cellular immunity of patients undergoing tonsillectomy were decreased in the early period and came to normal later. The cellular and humoral immune responses are stimulated in patients with tonsillitis, and tonsillectomy removes this stimulus without negatively affecting the patient's immune functions.

Antibody Formation↗

PubMed2003

First branchial cleft sinus presenting with cholesteatoma and external auditory canal atresia.

First branchial cleft abnormalities are rare. They may involve the external auditory canal and middle ear. We describe a 6-year-old girl with congenital external auditory canal atresia, microtia, and cholesteatoma of mastoid and middle ear in addition to the first branchial cleft abnormalities. Clinical features of the patient are briefly described and the embryological relationship between first branchial cleft anomaly and external auditory canal atresia is discussed. The surgical management of these lesions may be performed, both the complete excision of the sinus and reconstructive otologic surgery.

Abnormalities, Multiple↗

PubMed2003

The effects of dexamethasone, bupivacaine and topical lidocaine spray on pain after tonsillectomy.

OBJECTIVE: To compare the administration of bupivacaine hydrochloride, dexamethasone and lidocaine hydrochloride in decreasing post-tonsillectomy pain. METHODS: Eighty patients were enrolled in the study in ENT Clinic, Firat University, and in ENT Clinic Elaziğ SSK Hospital, Elaziğ (Turkey). Children between 6 and 14 years of age referred to our department for bilateral tonsillectomy for either recurrent tonsillitis or tonsillar hypertrophy. Data from 80 patients were analyzed. The first group had bupivacaine hydrochloride. The second group had dexamethasone infiltrated around each tonsil. The third group was given equal doses of 10% lidocain hydrochloride sprayed on the tonsillectomy fossa four times a day, and a placebo group received 9% NaCl applied to the tonsillar fossa four times a day. Pain scores, determined by visual analog scale, were obtained in the first, third and seventh postoperative days. RESULTS: Pain scores in the postoperative period were identical in the first, third and seventh postoperative days. According to VAS results the groups were compared on the basis of postoperative pain. In the first postoperative day, the difference between bupivacaine-placebo, dexamethasone-placebo and lidocaine-placebo groups was found to be statistically significant (P<0.05). Nevertheless the difference between bupivacaine-dexamethasone, bupivacaine-lidocaine and dexamethasone-lidocaine were not significant (P>0.05). In the third postoperative day, the difference between bupivacaine and lidocaine group found to be statistically significant (P<0.05). In the seventh post-operative day the results of bupivacaine, dexamethasone, lidocaine and placebo groups were similar (P>0.05). CONCLUSION: Bupivacaine, Dexamethasone and Lidocaine nasal aerosol decreased the pain significantly in the first postoperative day when it was compared with the placebo group. These three medicines can be used to reduce pain for children during the postoperative period applied tonsillectomy during the post-operative period. But lidocaine was more preferable, reducing pain in the third postop day better than bupivacaine.

Administration, Topical↗

PubMed2003

Free radicals and scavenging enzymes in chronic tonsillitis.

OBJECTIVE: This study aimed to define the relationship between chronic tonsillitis and levels of malondialdehyde and superoxide dismutase in free radical and antioxidant forms. It is suggested that free oxygen radicals may play a role in chronic tonsillitis. Materials and methods One hundred twenty-four patients were enrolled in the study. Tonsillectomy was performed via the usual dissection-snare method. Venous blood was taken preoperatively and at 2 weeks postoperatively. Blood samples and tonsil specimens were evaluated for malondialdehyde and superoxide dismutase analysis. RESULTS: The levels of malondialdehyde and superoxide dismutase in plasma were compared preoperatively and postoperatively, and there were statistically significant differences between these levels (P < 0.05). In contrast, the levels of malondialdehyde and superoxide dismutase in tonsil tissue were not correlated with the plasma levels of malondialdehyde and superoxide dismutase in pretonsillectomy and posttonsillectomy terms (P > 0.05). CONCLUSION: The presence of malondialdehyde and superoxide dismutase in plasma and tonsil tissue reinforces the involvement of oxidative stress in the pathophysiology of chronic tonsillitis.

Adolescent↗

PubMed2003

[The effect of vitamin E on histopathologic healing and lipid peroxidation levels in experimentally induced traumatic tympanic membrane perforations].

OBJECTIVES: To examine the favorable effects of vitamin E on tympanic membrane perforations induced mechanically in guinea pigs. STUDY DESIGN: Bilateral tympanic membrane perforations of 1.8 mm were induced in 40 guinea pigs. The animals were randomly divided into two groups equal in number. One group remained untreated, while the other was administered vitamin E (100 mg/kg/day) through intramuscular injections. On days 1, 3, 5, and 7, five animals in each group were randomly sacrificed. Histopathologic changes in the tympanic membranes were evaluated and malondialdehyde levels were determined. RESULTS: Significant increases were observed in epithelial thickness, fibroblastic proliferation, and neovascularization in the study group (p<0.05). Epithelial thickness was found to be increased in both groups beginning from the first day; however, this increase was more rapid in the study group. Although malondialdehyde levels showed significant increases on days 3 and 5 in both groups (p<0.05), they returned to the first day values in vitamin E-treated animals on day 7, whereas controls still maintained high malondialdehyde levels. CONCLUSION: Vitamin E hastens the healing process of traumatic tympanic membrane perforations.

Animals↗

PubMed2003

Bacteriemia in septoplasty and septorhinoplasty surgery.

This study was conducted in an attempt to investigate whether bacteriemia developed in patients with septoplasty and septorhinoplasty in the postoperative period during which an anterior nasal pack was in their nose. Fifty-three patients who went through septoplasty or septorhinoplasty operations were followed in this study. Nasal smear cultures were obtained from all the subjects before the operation. After the packs were retrieved, smears were also obtained from the pack material. Venous blood samples were obtained from the patients immediately before the operation, after the operation and immediately following the retrieval of the pack. When preoperative nasal smear cultures and postoperative pack material cultures of the patients that were obtained at 48 hours were compared, it was seen that different microorganisms were present in 66% of the patients. Bacterial growth was not observed in any of the preoperative blood cultures; whereas 8 patients (15.0%) had bacteriemia in the blood samples obtained immediately after the operation and 9 (16.9%) had growth in the blood samples obtained following the retrieval of the pack. With these results we have seen that bacteriemia can develop in patients with septoplasty and septorhinoplasty. It did not cause serious clinical problems. However, in patients with cardiovascular problems, this possibility should be taken into consideration and relevant preoperative precautions should be implemented.

Adult↗

PubMed2003

[An investigation of patients with acute peripheral facial palsy with regard to Lyme disease].

OBJECTIVES: We investigated the role of Borrelia burgdorferi in the etiology of idiopathic acute peripheral facial palsy. PATIENTS AND METHODS: Nineteen patients (15 females, 4 males; mean age 38 years; range 14 to 61 years) with acute peripheral facial palsy were studied. Following routine otolaryngologic examination, all the patients underwent taste, Schirmer, and stapedial reflex tests to evaluate the level of the palsy. Laboratory examination included routine biochemistry analysis, serum C-reactive protein, rheumatoid factor, and erythrocyte sedimentation rate. Anti-Borrelia burgdorferi IgM and IgG antibodies were sought by ELISA in venous blood samples. RESULTS: Of the patient group, acute facial palsy was localized on the right in 12 patients (63.2%), and on the left in seven patients (36.8%). None of the patients had bilateral involvement. No IgM or IgG seropositivity for Borrelia burgdorferi was detected in the serum samples. CONCLUSION: In highly endemic areas, it may be helpful to detect or even to eliminate Lyme disease through screening of serum in patients with acute peripheral palsy.

Acute Disease↗