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Biomedical subjects

Iu A Mazing

Publications and source records attributed to Iu A Mazing.

At least 19 recordsLinked to original sources
PubMed1992

[The antimicrobial cationic proteins of the neutrophilic granulocytes in experimental Q rickettsiosis].

Blood of 56 guinea pigs with experimental Q rickettsiosis was studied cytochemically (lysosomal cationic test) to measure the level of cationic proteins in neutrophil granulocytes. Development of Q rickettsiosis resulted in a decrease in the killing ability of neutrophils, depending on infection dose introduced. However, by day 7 of the disease, the level of cationic proteins in blood neutrophil granulocytes returned to the initial range. Similar situation was noted after subcutaneous injection of Coxiella burnetti corpuscular antigen. Subcutaneous infection with the living culture stimulus induced the wave-like decrease of the cationic proteins content. Infection of pre-immunized animals led to smaller decrease in the cationic proteins levels and to their more rapid recovery. Aspects of antimicrobial activity of neutrophil granulocyte cationic proteins in experimental Q rickettsiosis is discussed.

Animals↗
PubMed1991

[Neutrophilic granulocytes and host defense systems].

The review shows that neutrophil granulocytes play a key role in the host defence against infection diseases. An important role belongs to the antibacterial cation proteins. Their study allows to create new methods of rapid diagnosis of the host resistance. The use of one of such tests (lysosome-cationic one) showed its high efficiency in the assessment of the disease gravity and the prognosis of complications.

Humans↗
PubMed1990

[Effects of impulse hydrodynamic influences on blood cells in vitro and in vivo].

Impulse hydrodynamic influences (IHDI) resulted in an increase of accumulation of acridine organ, degranulation of neutrophils, decrease in adenylate cyclase and Na+, K+, Mg2(+)-dependent ATPase activities in the white blood cells of man and rat in vitro and in vivo. It was found that the cell's response to one-fold IHDI was more pronounced than to 5-fold IHDI. It was suggested that in certain conditions IHDI could lead to a decrease in reactivity of white blood cells.

Acridine Orange↗
PubMed1990

[Age-related immunodeficiencies of the neutrophilic granulocyte system].

Transitory age-associated deficiency of the granulocyte cationic proteins as a cause of a phagocytosis disturbance, loss of the phagocyte cell ability to kill phagocytosed bacteria is established. Quantitative indexes of myeloperoxidase content, elastase and catepsin G in the neutrophil granulocytes of newborns and laboratory animals are determined. Hypothesis is developed on the transitory age-associated immunodeficiencies of the neutrophil granulocyte system as one of the endogenic risk factors in the development of infectious diseases during early postnatal period.

Aging↗
PubMed1990

[Effect of defensin on the process of healing of aseptic skin wound and on the permeability of blood vessels].

It was shown, for the first time, that polypeptide of rabbit's neutrophils, defensin (D) has the ability to accelerate the reparation process (RP). D was infused intramuscular to rats (125 micrograms/kg) 2 days before the operation, then the skin on the back was dissected through all layers (the length of the wound was constant-10 mm). The rate of the RP was estimated by the changes of the wound length on 2, 5, 7, 13-15 days after the operation. The injection of D or physiological solution was continued during 2 weeks. It was shown, that beginning from the fifth postoperative day the RP in the experimental rats developed faster, than in control rats. In the experimental group the full reparation appeared earlier, than in the control group (on 21-22 and 24-26 days correspondingly. Qualitative (with trypan blue) and semi-quantitative (with colloid coal) methods have shown, that D augmented the permeability of blood vessels. It was proposed, that the RP acceleration was induced by D augmentation of the blood vessels permeability.

Animals↗
PubMed1989

[A method for evaluating a diagnostic inflammatory focus by using the "cold test"].

Clinico-morphological test for the quantitative evaluation of exudative and cell response in the diagnostical inflammatory focus in various forms of human pathology is developed. The values in practically healthy persons are established. The possibilities of the test use for the prognosis of disease and the development of postoperative complications in patients with cold trauma are shown.

Adult↗
PubMed1988

[Effect of UV radiation and the autotransfusion of UV-irradiated blood on the content of cationic proteins in the neutrophilic granulocytes of calves].

A study was made of the influence of UV-irradiation (254 nm) of blood in vitro, of the autotransfusion of UV-irradiated blood (AUVIB), and of the mixture of UV-irradiated and intact blood in vitro on the content of bactericidal cation proteins (CP) in blood neutrophil of calves suffered from dyspepsia and broncho-pneumonia. Age differences were noticed in CP contents and their decrease in neutrophils following AUVIB in vivo and administration of the mixture of blood in vitro. The decrease in cell CP contents is presumably due to neutrophil degranulation and CP release into the blood plasma. Since the initial mechanisms of neutrophil degranulation are located on the cell surface, the CP release is supposed to result from a membranotropic effect of UV-irradiated blood on the intact autologous blood. This effect may explain the increase in nonspecific resistance of organism after the AUVIB, being one of the main therapeutic phenomena of the AUVIB-therapy.

Animals↗
PubMed1988

[A method for assessing the lysosomal secretory activity of neutrophilic granulocytes using a diagnostic focus of inflammation].

Lysosomal secretion of neutrophilic granulocytes has been studied in normal subjects (healthy males and females aged 20-50), pregnant women and burnt patients. A significant rise in lysosomal secretory activity of neutrophilic granulocytes was recorded in the 3rd trimester of pregnancy. Low lysosomal secretion can serve an indicator of poor prognosis in patients with a severe burn trauma.

Adult↗
PubMed1987

[Express evaluation of the severity of the course of lactation mastitis].

The authors consider that quantitative and qualitative characteristics of phagocytosis can be given with the help of lysosomal-cationic test (LCT) at different periods of acute purulent lactation mastitis. LCT gives prognosis of the course of the disease and a reliable estimation of the treatment efficiency.

Blood Bactericidal Activity↗
PubMed1986

[Method of staining for lysosomal cationic proteins in histological paraffin sections of biopsies].

A method is suggested for a histochemical and immunofluorescent detection of lysosome cation proteins in the histologic paraffin sections of biopsies. The method is based on the staining of histologic sections obtained from the material fixed in glutaraldehyde buffered with an alcohol solution of a fast green (pH 8.1-8.2) and on the detection myeloperoxidase and lactoferrine by means immune serum by Coons's method. 0.25% solution of azur A (B, C) is used for staining the cell nuclei and viable bacteria.

Biopsy↗
PubMed1986

[Detection of cationic proteins and peroxidase in semithin sections].

A method of histochemical identification of cationic proteins, peroxidase, viable and killed bacteria in semithin sections of biopsy samples is suggested. The method is based on dying of araldite-embedded semithin sections with a buffered alcohol solution of fast green (pH 8.15) and on peroxidase identification with O-dianisidine. The method suggested can be used for distinguishing occult forms of infectious gastritis, evaluation of secretory and barrier function of gastric epithelium, selective dying of leucocytes in tissues, revealing of viable and non-viable bacteria in the infective foci.

Adult↗
PubMed1985

[Isolation and physico-chemical properties of lactoferrin from swine neutrophils].

Lactoferrin, a non-heme iron-binding protein was isolated from pig neutrophils. The purification procedure included initial extraction of the protein in the presence of cetyltrimethylammonium bromide followed by chromatography on carboxymethyl-cellulose and Sephadex G-100. The thus obtained protein was found to be homogeneous on polyacrylamide gel (PAAG) electrophoresis at acidic values of pH. PAAG electrophoresis in the presence of sodium dodecyl sulfate revealed a single component with a molecular weight of 75 000-80 000. The resulting protein is capable of binding two atoms of iron molecule. The absorbance spectra for the pig neutrophil lactoferrin are identical to those for cow milk lactoferrin in the visible region and have a maximum at 465 nm. The amino acid composition of pig lactoferrin was determined. Isoelectric focusing of the protein obtained in a PAAG stabilized pH gradient revealed a component with pI of about 6.8. A single precipitin line was observed with rabbit antipig lactoferrin when examined by immunodiffusion. No immunological cross-reactions were observed between pig lactoferrin and bovine lactoferrin.

Amino Acids↗