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Iu A Popov

Publications and source records attributed to Iu A Popov.

At least 19 recordsLinked to original sources
PubMed2004

[Analysis of genomic polymorphism of typical and atypical strains of the plague pathogen using polymerase chain reaction with universal primers].

An analysis of genome polymorphism of the Y. pestis strains by using the method of polymerase chain reaction (PCR) for the tandem repeats of bacteriophage M13 DNA revealed a species similarity of both typical and atypical (according to diagnostic signs) plague-microbe strains. Strain Y. pestis A-1726 with the atypical differential-and-diagnostic properties, without the amplicon specified for Y. pestis and sized 1000 b.p., was identified among 27 analyzed Y. pestis strains. The amplicon profiles of the basic Y. pestis subtype were found to be different from such profiles of other Y. pestis subtypes.

Bacteriophage M13↗
PubMed1999

[Comparative study of the structure of some genetic probes used for typing Yersinia pestis strains].

A nucleotide sequence common for genetic probes used for detection and investigation of Y. pestis strains MK, IS100, and HRSIII was identified on the basis of restriction, hybridization, and computer analysis. This region of chromosomal DNA is a part of low-molecular BX-probe (about 170 bp) we have developed. The results of genomic fingerprinting of Y. pestis strains by the BX-probe are promising as regards its future utilization for typing the strains isolated in various endemic areas.

Base Sequence↗
PubMed1998

[Molecular genetic analysis of DNA structure of pFra plasmids in plague pathogen of varying biovar].

Data on comparative molecular genetic analysis of pFra plasmids from plague bacillus belonging to either two biovars (antiqua and orientalis) are presented. It was established that during evolution these replicons were rearranged, which resulted in the differences between pFra plasmids of plague bacillus of antiqua biovar (Yersinia pestis 231 and Y. pestis 358/12) and that of orientalis biovar (Y. pestis A1122 and Y. pestis EV). These included inversions of two plasmid DNA regions and size differences of 3 kb.

Evolution, Molecular↗
PubMed1996

[Genetic research on plague pathogen in plague control].

The results of molecular-genetic studies performed by Russian specialists in plague research are discussed. On their basis, new concepts concerning the factors determining virulence of the plague bacterium were formulated, and certain aspects of Yersinia taxonomy were clarified.

Genetic Engineering↗
PubMed1996

[Phenotypic expression of the Ca2+-dependence trait in recombinant cells of Yersinia pestis (Lehmann, Neumann)].

A conceptual model of the Yersinia pestis Ca(2+)-dependence mechanism is proposed. The model is based on data from analyses of peculiarities of recombinant cells of this plague-causing agent carrying the cloned first Bg/II fragment of the Ca(2+)-dependence plasmid (pCaD) and a combination of this fragment and other plasmid pCaD fragments. The data obtained also allowed a revision of the role of the lcr GVH locus of pCaD in this phenomenon.

Calcium↗
PubMed1994

[Improvement of a method for detecting of strains of the plague microbe using polymerase chain reaction].

Three pairs of oligonucleotide primers, complementary to nucleotide sequences of Yersinia pestis plasmids (pPst, 9.5 kb; pCad, 70 kb; pFra, 95 kb), were used in polymerase chain reaction for high-sensitive specific detection of plague pathogen. Primer pairs P1,P2,C1,C2, and F1,F2 were used to amplify fragments of pla gene (plasmid pPst), yop1 gene (plasmid pCad of plague microbe), and caf1 gene (plasmid pFra), respectively. The method developed enables specific detection of strains from all natural loci in Russia and contiguous states as well as strains of oceanic origin. Sensitivity of method is 50-100 CFU/ml. Primer sequences enable to amplify gene fragments, located in three own plasmides of plague microbe, in the same reaction mixture. The method offers identification of plague microbe and determination of its virulence and epidemic significance.

Base Sequence↗
PubMed1993

[Design of a chromosomal DNA probe species specific for Yersinia pestis].

A 14.8 kb DNA fragment from the chromosome of Yersinia pestis TWJ was cloned and the restriction map constructed. The fragment designated as T16 and its subfragments were tested in dot-hybridization with strains of Yersinia genus and other members of Enterobacteriaceae. A species-specific DNA probe (designated MK) was constructed on the basis of the T16 fragment. As judged from restriction analysis, blot-hybridization experiments and, partially, sequencing, significant homology exists between the MK DNA probe and this one developed by Bardarov et. al. (1990). A repeated sequence in two copies was discovered in the MK fragment.

Base Sequence↗
PubMed1991

[Design of a species-specific DNA probe based on the pFra plasmid of the plague pathogen].

The recombinant plasmid pBS1 carrying a 2 kb SalGI fragment of Yersinia pestis pFra plasmid was constructed by insertion of the fragment into a vector plasmid pBR327. SalGI-BspRI 400 bp subfragment was recloned into a pBR322 vector plasmid. Open reading frame was found in the fragment by DNA sequencing technique. The subfragment designated F1-probe permits one to identify specifically the Yersinia pestis strains harbouring pFra plasmid, thus, differing them from closely related Yersiniea and other representatives of Enterobacteriaceae family.

Amino Acid Sequence↗
PubMed1991

[Constructing a test system for the identification of plague microbe based on genetic probes].

DNA probes for detection of the plague agent Yersinia pestis were made on a basis of its three typical extrachromosomal replicons. The recombinant plasmid pBS2 including pBR327 vector and SalGI-BspRI fragment of the plasmid pFra was constructed. The above fragment is connected with synthesis of Y. pestis capsular antigen and it is a 400 bp species-specific DNA probe called F1 which is suitable for identification of Y. pestis species that bears the 60 mdal plasmid. The DNA probes called P1 was made on a basis of the plasmid pPst; it is the 460 BglII-BamHI fragment of the fibrinolysin-coagulase gene suitable for species-specific detection of Y. pestis species that bears the 60 mdal plasmid. The P1 fragment was cloned into the pAT153 vector and the constructed recombinant plasmid was called pEK7. The recombinant plasmid pCL1, including the pBR325 vector and the 6th BamHI fragment of Y. pestis EV plasmid pCad was constructed. The above fragment includes the replication origin of the pCad and it is hybridized to the pCad-bearing strains of Y. pestis and Y. tuberculosis only. Thus, it may be a basis for a bi-species-specific DNA probe making. These three recombinant plasmids are considered as a test-system for detection of both typical and atypical strains of Y. pestis.

Bacteriological Techniques↗
PubMed1989

[The development of a method of nitrosoguanidine inactivation in the mutagenic treatment of bacteria].

A simple method for the inactivation of nitrosoguanidine, a mutagenic agent permitting the simplification and acceleration of the procedure used for obtaining bacterial mutants, is described. This method is based on the addition of unithiol to nitrosoguanidine solution to a final concentration of 0.25-0.5% and the subsequent normalization of the pH of the medium with 0.1 N NaOH solution.

Bacteria↗
PubMed1989

[Non-operative sanitation of suppurative-septic foci].

The authors made an analysis of their experience with treatment of 63 patients with abscessing pneumonia and gangrene of the lungs, 137 patients with peritonitis, 27 patients with gynecological sepsis, 80 patients with pyo-necrotic foci in lower extremity tissues. They used the method of regional (intraaortal, intraarterial) infusions of drugs which is thought to open new possible ways of nonoperative treatment of pyoseptic foci of various localizations.

Anti-Infective Agents↗
PubMed1988

[The functional properties of thrombocytes in patients with suppurative peritonitis].

An examination of 49 patients with local and diffuse purulent peritonitis at different stages of surgical treatment has shown that the pyodestructive process in the abdominal cavity develops against the background of thrombocytosis, thrombocyte destruction, their decreased energy resources and tendency to retarded and irreversible aggregation. To correct the disturbed aggregation properties of thrombocytes the HBO and intraaortal infusions of antiaggregants and vasoactive drugs may be used.

Adolescent↗
PubMed1986

[Hemodynamics in the region of the visceral branches of the abdominal aorta in diffuse suppurative peritonitis].

Hemodynamics of the region of visceral branches of the abdominal aorta was investigated in 75 patients (40 patients with diffuse peritonitis and 35 patients without any pathology of the abdominal organs as a control group). In patients with diffuse purulent peritonitis the volume blood flow in the celiac trunk, mesenterial and renal arteries was reliably reduced, the conditions for the development of acute hepato-renal insufficiency being made.

Adult↗
PubMed1983

[Detection and characterization of the plasmids of the plague microbe which determine the synthesis of pesticin I, fraction I antigen and "mouse" toxin exotoxin].

Plasmid DNA was isolated from Yersinia pestis strains containing pesticin I or fraction I antigen and "mouse" toxin determinants. Specificity of DNA preparations was studied by using them for transformation of plague agent strains carrying no plasmids. pPstI plasmid (molecular weight 7,0-7,8 MD) encoded pesticin I, fibrinolysin and plasmacoagulase synthesis. Fraction I antigen and "mouse" toxin production determinants were borne on pFraI/Tox plasmid (molecular weight about 50 MD). The observation that some Y. pestis cultures, having lost the ability to synthesize one of pFraI/Tox products, still retained this plasmid in their cells, is regarded as an evidence for a complicated regulation of pFraI/Tox function.

Antigens, Bacterial↗
PubMed1976

[Study of the membranes of pigment-free mutant of Staphylococcus aureus].

The membranes from pigmented strain of Staphyloccus aureus 209-P and its apigment mutant B-6 were isolated. The infrared spectra, the cytochrome content, and the activities of the dehydrogenases and oxidases of the membranes were studied. The apigment mutant membranes differed from those of the pigmented strain in all parameters studied. It is concluded that the carotenoid pigments affect the chemical composition and functional activity of the Staphylococcus membranes.

Carotenoids↗