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Iu A Revazova

Publications and source records attributed to Iu A Revazova.

At least 19 recordsLinked to original sources
PubMed2006

[A complex approach to evaluation of human genome instability].

In this study, evaluation of genome instability in individuals exposed to chemical compounds included detection of the genetic polymorphism of some xenobiotic metabolic enzymes (CYP1A1, CYP1E1, PON1, GSTM1, GSTT1), as well as measurement of oxidative state chemiluminescent variables and the level of cytogenetic damage. According to the study, the level of chromosomal aberrations in peripheral blood lymphocytes shows a strong correlation with PON54 left allele and GSTM1 null genotype, and can be described by the polynomial function of blood plasma luminol-dependent chemiluminescence. The frequencies of micronuclei in buccal epithelium displayed a weak association with GSTT1 null genotype.

Genome, Human↗
PubMed2006

[The congenital morphogenetic variants and genetic polymorphism of the system of xenobiotic detoxication in children living in dioxin-contaminated regions of South Vietnam].

The presence of dioxins and dioxin-containing ecotoxicants (DCE) in human environment leads to multi-level homeostasis disbalance and, eventually, dioxin pathology (DP) develops. This was demonstrated for the adult population of South Vietnam (1), who had suffered from the use of military chlorphenoxiherbicides to which dioxins had been added. The subjects of this study were children who belonged to the second generation of the inhabitants of DCE-polluted South Vietnam areas. In this sample, the frequency of malformations depending on two factors, genes GSTMI, GSTT1, CYP1A1 genotypes, and DCE exposure, were estimated. The study demonstrates that an increased level of congenital morphogenetic variants per one child is associated with the presence of DCE in the environment, as well as the fact that this parameter is influenced by genotypes of xenobiotic detoxication genes.

Alleles↗
PubMed2004

[Active oxygen forms and genome damage by environmental factors].

The role played by active oxygen forms in damaging the human genome by environmental factors as well as the application of antioxidants to keep the oncology statistics among the hazardous-area residents low are under discussion. According to the independent results, there are essential differences between mechanisms of inflicting a genetic lesion to healthy subjects and to individuals of the risk categories due to that the latter have contacts with high concentrations of chemical compounds and with clinical patients with developed free-radical pathologies.

Environmental Exposure↗
PubMed2001

[Genetic approaches to evaluating safety of human environmental factors].

Main achievements made in genetic toxicology in the past 40 years are analyzed. Guidelines for estimating gene toxic agents have been developed and a database pertaining to the mutagenicity of several thousands of chemicals has been set up. Experience in evaluating human mutagenic effects has been accumulated. It is necessary to develop a current toxicological and sanitary classification of mutagenic xenobiotics, to take a stock of environmental gene toxic agents by their activity and pollution in the areas of Russia, to work out criteria for the actual genetic hazards (a genetic risk) of mutagens entered the human habitat, to study a human individual response to mutagenes and to make "a genetic certification", and to analyze the feasibility of human genome protection from environmental mutagenic factors.

Environmental Exposure↗
PubMed1995

[Study of antimutagenic properties of the citrus drink Tampico].

Oral administration of the orange drink Tampico containing additionally vitamins A (12.7 mg/l), B (1.81 mg/l), C (86.9 mg/l) to FICBAxC57B1/6 mice has been shown to stabilize erythrocytic membranes and reduce chromosomal aberrations in the murine bone marrow cells induced by cyclophosphamide. There are experimental data on the effects of Tampico on the rate of reparative synthesis in peripheral lymphocytes and blood levels of malonic aldehyde in the mice exposed to model psychoemotional stress.

Animals↗
PubMed1993

[The pharmacokinetic modification of mutagenesis].

The mutagenic activity of some antitumor agents and the antituberculous agent isoniazid was studied by using some methods for estimating mutations on the Drosophila, mammals, and human cells. It was shown that mutagenic effects could be modified by adding various auxiliaries to the formulations of the drugs. The mechanisms of modifications were examined by pharmacokinetic techniques.

Animals↗
PubMed1993

[The antimutagenic action of a preparation of a species-nonspecific interferon].

The human gene-engineering gamma-interferon agent gammaferon (GF) is demonstrated to lower the level of chromosomal aberrations and release of cyclophosphamide-induced micronuclei in murine bone marrow cells. A model is suggested for regulating the ratio of single to multiple chromosomal aberrations induced by cyclophosphamide by altering the antioxidative status of the body. With this model, it was shown that the antimutagenic effects of GF were caused by two factors: the antioxidative effect of the corresponding placebo and the unknown (but none antioxidative) effect of gamma-interferon itself. Erythrocytic resistance to in vitro oxidative disturbance in the Fe2+/system was used as an integral measure of the body's antioxidative status.

Animals↗
PubMed1993

[Prolonged psychoemotional stress as an inducer of mutations in mammals and as a modifier of mutagenesis].

The present study was made to determine the genotoxicity of psychoemotional stress (PES) alone and its possible influence on chemical mutagenesis under combined treatment. Stress was registered by the classical parameters: 1) weights of the thymus and adrenal; 2) stomach and duodenal mucosa conditions. The unscheduled DNA synthesis (UDS) measurement in peripheral blood lymphocytes with simultaneous determination of chromosomal aberrations (CA) and micronuclei (MN) levels in bone marrow cells were applied for estimation the mutagenic activity. The results indicated that PES alone, as CP, induced UDS in peripheral blood lymphocytes. Intensity of UDS was dependent on the stress duration. The UDS kinetics in the lymphocytes from stressed mice and stressed mice treated with CP were different. PES and CP induced the similar amounts of CA in bone marrow cells. Value of CA had a comparable tendency with results of UDS. The combined effect of PES and CP in both tests was different from additive one. The effects in NM test were statistically significant only in case of combined PES and CP action. The mutagenic potency of PES and its role in the modification of environmental mutagenesis will be discussed.

Animals↗
PubMed1992

[The evaluation of the mutagenic activity of pertussis vaccinal preparations].

Two vaccine preparations obtained from Bordetella pertussis, whole-cell vaccine constituting one of the components of adsorbed DPT vaccine and acellular vaccine, were tested for mutagenicity. The doses of the preparations covered the range 1-100 ED50. Ames' test and the metaphase analysis of marrow cells of C57BL/6J mice were used. The acellular preparation was also tested on thymectomized mice, taking into consideration chromosomal aberrations in marrow metaphases. Whole-cell and acellular pertussis vaccines did not induce mutations in Salmonella typhimurium and chromosomal aberrations in mouse marrow cells.

Animals↗
PubMed1990

[Genetic determination of spontaneous and induced dominant lethality in Drosophila].

Estimation of heritability ha2 index in experiments on spontaneous and induced dominant lethality gives the possibility to evaluate with sufficient accuracy genetic determination of the effect and its dependence on casual reasons. Besides, the individual breeding method gave quite stable data both on late embryonic lethality index and on heritability value. Directed selection of different test-objects (species or stocks of Drosophila) should be carried out using heritability index, since selection efficiency mostly depends on this value degree. Making stocks resistant and sensitive to certain mutagen classes, their comparison from the point of view of their pharmacogenetic features, on the basis of the indices used, may be taken as the start of studies aimed at further identification of certain biochemical factors or system taking part in manifestation of mutagenic effect. The data obtained permit us to suggest an idea of advisability of studying the genetic control of mutagenesis in humans using the method described.

Animals↗
PubMed1983

[Experimental analysis and planning using the Salmonella/microsomes test].

A possible statistical treatment of the results obtained in Salmonella typhimurium TA1950, TA1535, TA1537, TA1538, TA98 and TA100 using the Salmonella/microsomes test was investigated. An analysis of independent repeated experiments pointed to the divergence of the data obtained. Consequently, it has been recommended to carry out the statistical treatment of experimental data within the limits of dispersion analysis with the subsequent use of the Scheffe's method for multiple comparisons. To stabilize the dispersion of experimental data, it is suggested to express the number of revertant colonies as lnX. A minimal volume of the data sample needed for resolving experimental tasks has been calculated. In standard experiments aimed at revealing the mutagenic activity of the compounds used, three Petri dishes should be used in each variant of experiments.

Microsomes↗
PubMed1982

[Indices for assessing genetic damage induced by chemical compounds. Assessment of the induction of sister chromatid exchange by chemical compounds].

Some preferences of using the new index "number of chromosomes involved in the exchange" in comparison with the standard index "number of SCE per cell" are discussed. The mean probability of the induced involving a chromosome into the exchange is estimated as (formula: see book) where pt and pc are the mean number of chromosomes involved in the exchange divided to the total number of chromosomes in the test and control groups, respectively.

Animals↗