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Biomedical subjects

Iu L Kaminskiĭ

Publications and source records attributed to Iu L Kaminskiĭ.

14 recordsLinked to original sources
PubMed1989

[The relationship between the intracellular content of 1-methyl-3-isobutylxanthine and the permeability for water of the bladder wall in the frog].

The data obtained show a direct interrelationship between the concentration of MIBX in cells and an increase of osmotic permeability. The method of determination of intracellular concentration of the MIBX using [3H]-MIBX, was developed, and the way to obtain radioactive MIBX described. The used approach permits to take into consideration functional changes as related to the intracellular concentration of inhibiting agents.

1-Methyl-3-isobutylxanthine↗
PubMed1988

[Synthesis of nucleosides labeled with tritium at the 5'-carbon atom of the ribose residue].

Chemical and enzymatic syntheses of [5'-3H]adenosine, [5'-3H]guanosine, and [5'-3H]uridine have been developed. The reduction of beta-D-ribo-pentadialdo-1,4-furanosyl derivatives of corresponding bases is used in the chemical synthesis. The maximum molar activity of the labelled products was 220 TBk/mol in reactions with [3H]NaBH4 and 370-740 TBk/mol in reactions with gaseous tritium. The enzymatic synthesis was performed by the rebosylation of heterocyclic bases with nucleoside phosphorylase and [5'-3H]uridine as a ribosyl donor. Nucleoside phosphorylase is proposed to be used in the immobilized form to avoid the decrease of molar activity. Nucleosides labelled with tritium both in ribosyl and heterocyclic moieties were synthesised enzymatically.

Isotope Labeling↗
PubMed1979

[Fractionation of nucleosides and nucleoside-5'-mono-, di and triphosphates by thin layer chromatography in silufol].

New solvent systems--dioxane--isopropanol--water--ammonium (4:2:4:1) and dioxane--water--ammonium (6:4:1)--for rapid qualitative and quantitative estimation of nucleosides, nucleoside-5'-mono-, di- and triphosphates by thin layer chromatography is Silufol UV-254 are proposed. The Rf values for 40 ribo- and deoxyribonucleotides of these systems are given.

Chromatography, Thin Layer↗