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Iu M Azizov

Publications and source records attributed to Iu M Azizov.

13 recordsLinked to original sources
PubMed1991

[Acid-soluble fractions of the blood in patients with nonspecific diseases of the respiratory organs].

A method permitting the differentiation between the types of pulmonary inflammatory processes in patients with nonspecific diseases of the respiratory organs by the parameters of blood plasma acid-soluble fraction is suggested. If the inflammation is of an infectious origin (pneumonia, chronic bronchitis), the ratio of low-molecular to high-molecular fractions of the blood plasma acid-soluble fraction is 4.2 +/- 1.8. In allergic inflammations (bronchial asthma) this ratio is 9.0 +/- 5.8. The results of this test were in line with clinical laboratory and x-ray findings in 86.7% of the examinees. In complex with other methods of examination, the described technique may be used for the differential diagnosis of allergic and infectious inflammations in patients with respiratory diseases.

Acute Disease↗
PubMed1979

[Tissue specificity of the decrease of cattle lymphocyte DNA methylation during chronic lymphoid leukemia].

It has been found that the content of m5C in the DNA preparations tested have been revealed. The DNAs from normal and leukemic lymphocytes of blood, lymphonodi and spleen differ in ther acceptor ability in the reaction of heterologous methylation in vitro, induced by DNA-methylase from Enterobacter cloacea in the presence of [3H-methyl]S-adenosyl methionine: the ratio of radioactivities in methylated cytosine and adenine residues (m5C/m6A) in leukemic lymphocyte DNA is much lower than in healthy animals' lymphocytes. The decrease in the methylation of DNAs from various lymphoid organs of animals with chronic lymphoid leukemia is well correlated with the impairment. No significant changes of the m5C level and the acceptor ability of the in vitro reaction of heterologous methylation of cow lymph lymphocyte DNA have been observed. The data obtained may be interpreted in terms of tissue (cell) specificity or differences in the degree of DNA methylation under conditions of chronic lymphoid leukemia. It is assumed that the changes in DNA methylation may underlie the disturbances in the regulation of activity of the leukemic cell genetic mechanisms.

Animals↗
PubMed1979

[Ganglioside composition of normal and leukemic bovine lymphocytes].

The ganglioside composition of bovine peripheral lymphocytes was shown to change sharply under lymphoid leukemia. In normal lymph, lymph nodes, spleen and blood lymphocytes the major ganglioside is N-glycolylhematoside, whereas in calf thymus lymphocytes appreciable amounts of more polar components (GM1- and GD1a-like gangliosides) were found. In leukemic lymphocytes isolated from the same tissues the hematoside content is decreased, while the amount of more polar gangliosides is increased. Possible causes of the altered ganglioside pattern in leukemic lymphocytes are discussed.

Animals↗
PubMed1979

[Intragenome distribution of 5-methyl cytosine and reassociation kinetics of cow blood lymphocyte DNA under normal conditions and in chronic lymphoid leukemia].

The DNA from cow blood lymphocytes is methylated in a varying degree: the maximal content of 5-methyl cytosine (2,3 mol%) is found in the "instantly" renaturating sequences (Cot lett than 10(-4)), a relatively large amount (1,4 mol%)--in moderately repeated sequences (Cot = 10(-4)--400) and the minimal amount (0,9 mol%) in the unique sequences (Cot greater than 400). In their reassociation kinetics, GC-content and other physico-chemical properties the blood lymphocyte DNA of the controls and of animals with chronic lymphoid leukemia appear to be similar. Consequently, the genome organization of leukemic animals does not change significantly; a considerable decrease of 5-methyl cytosine of lymphocyte DNA in lymphoid leukemia parallels the decrease of genome methylation in the leukemic cells. This decrease does not affect the unique sequences, but involves all types of repeated sequences (moderately and frequently repeated ones and palindromes). It is assumed that the specific disturbances in genome methylation under lymphoid leukemia may be a cause of transcription deficiences and cell transformations.

Animals↗
PubMed1978

[Phospholipid composition of lymphocytes from normal and leukemic blood, lymph, lymph nodes and spleen of cattle].

The phospholipid composition and cholesterol content of lymphocytes from bovine blood, lymph, lymph-nodes and spleen were studied. The cholesterol/phospholipid molar ratio in the normal lymphocytes of blood and spleen was found to be about two times higher than that in the normal lymphocytes from lymph and lymph-nodes. On lymphocytic leukemia the cholesterol/phospholipid molar ratio of the blood lymphocytes decreased sharply, whereas that in lymph and lymph-nodes lymphocytes was unaffected.

Animals↗
PubMed1978

[Changes in the specificity of DNA methylation in cattle blood lymphocytes under chronic lymphoid leukemia].

Under conditions of chronic (spontaneous) lympholeucosis the amount of 5-methylcytosine in cattle blood lymphocyte DNA is decreased approximately by 30%. No other changes in the DNA (e. g. GC-content, Tm, amount of pyrimidine sequences differing in their lengths and composition) were observed. Thus, the decrease in the amount of 5-methylcytosine in lymphocyte DNA is due to a decrease in DNA methylation. This decrease is non-random and involves mainly the Pu-m5C-Pu sequences without affecting the long pyrimidine blocks. In nuclear extracts from lymphocytes of healthy animals the DNA-methylase activity having an optimum at pH 6,0 was found; the DNA-methylase activities found in the nuclear extracts of leukaemic cow lymphocytes had their optima at pH 5,5 and 7,5. In vitro the DNA-methylase activities of leukaemic lymphocytes nuclei methylate the cytosine residues of DNA in other sequences than enzyme(s) of the extracts from normal lymphocyte nuclei. Changes in the pattern of DNA-methylase activities as well as the decrease and distortions in the character of DNA methylation may underlie the disturbances in the regulation of transcription of genome and cause the transformation of cells under conditions of lympholeucosis.

Animals↗
PubMedDate not supplied

[The protein components of the acid-soluble fraction of normal human blood plasma and in peritonitis patients].

Samples of acid-soluble blood plasma fractions from 10 healthy donors and 12 patients with peritonitis have been fractionated, using the techniques of vertical electrophoresis in polyacrylamide gel and high pressure liquid chromatography. Three basic protein-containing components with the molecular mass 43, 30 and 14 kD have been identified in samples of donor blood plasma. In peritonitis there is an increase in the level of some fractions present in acid-soluble fractions of donor blood plasma and the generation of new protein-containing acid-soluble components. A band corresponding to a component with a molecular mass 64 kD and a wide band in the range of 9-14 kD have been registered. The treatment of donor blood with trypsin generates some components characteristic of acid-soluble blood plasma fraction of patients with peritonitis. The authors believe that increased proteolytic activity is one of the factors responsible for qualitative and quantitative changes in acid-soluble blood plasma fraction in peritonitis.

Adult↗
PubMedDate not supplied

[Correction of disorders of tissue perfusion in surgical patients in critical conditions and their evaluation by changes in the indices of the acid-soluble fractions of blood plasma].

Rheopolyglucin infusion and vasodilation therapy with droperidol have been performed in 16 critically ill surgical patients with low O2 tissue utilization with the aim in view to assess tissue perfusion disturbances and normalize peripheral blood flow. Basic central hemodynamic parameters and oxygen transport system parameters have been determined. Using gel filtration, acid-soluble blood plasma fractions have been studied spectrophotometrically. Despite the fact that rheopolyglucin infusion caused an increase in oxygen flow index (OFI) at the expense of cardiac index (CI) growth, O2 extraction remained practically unchanged. Vasodilation therapy, with slight changes of CI and OFI, caused an increase in O2 consumption and arterio venous difference, as well as a reduction of mixed venous blood pO2, this accompanied by an increase in acid-soluble blood plasma fractions, especially second fraction at 255 nm. A direct correlation has been established between basic oxygen transport system parameters, characterizing O2 tissue utilization and changes of acid-soluble blood plasma fractions parameters. The changes in acid-soluble blood plasma fractions parameters may serve one of the criteria characterizing the efficacy of procedures aimed at the improvement of tissue perfusion.

Adult↗
PubMedDate not supplied

[Structural transitions in DNA, isolated from normal and neoplastic cells].

A decrease of temperature transition in spin-labeled DNA isolated from leucose cells compared with normal cells is found. It is shown by kinetic formaldehyde method that it may be the result of many defects in the secondary structure of macromolecule in DNA1. The spin label method is shown to be used for the analysis of DNA macromolecule damaged with small irradiation doses. Various radiosensitivity of DNA isolated from the blood of normal and leucose cells is observed.

Animals↗