[Determination of bacterial peroxidase activity by using methylpara-aminophenol sulfate].
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Biomedical subjects
Publications and source records attributed to Iu M Fel'dman.
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A rapid method for determination of microbial sensitivity to antibiotics and chemotherapeutic drugs with the use of the peroxidase test is described. The procedure takes 6 hours. Peroxidase is determined by a change in the color of the methyl-para-amino phenol sulfate solution added to the broth culture in 6 hours (simultaneously with hydrogen peroxide). The peroxidase test provides detection of the microbe multiplication even when no turbidity is observed.
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On the basis of analysis of literature and personal observations and studies the authors explain a number of epidemiological and clinical peculiarities of intestinal infections with various pathogenic doses of the causative agents; under the term pathogenic dose is understood the minimal amount of microbes necessary for the onset of the disease with the natural mechanism of transmission of the infection. Regularities of the effect of the pathogenic dose of the causative agent on the epidemic process common for intestinal infections were formulated.
It is recommended to use the capacity of pathogenic staphylococci to be lysed by polyvalent therapeutic staphylococcal bacteriophage in the capacity of an additional simple and accessible criterion of staphylococcus pathogenicity. Of 147 strains of the pathogenic plasmacoagulating staphylococci 101 were lysed by the phage and of 166 nonplasmocoagulating nonpathogenic strains--only 6. This test correlated with the other signs of staphylococcus (lecithinase and hemolytic activity). The simplicity and sufficient specificity of this test permits to use it in any practical laboratory. Polyvalent diagnostic phage can be used on the basis of therapeutic bacteriophage by its additional adaptation to the pathogenic strains of staphylococcus.
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A method for bacterial culture storage and transportation is suggested consisting in impregnation of filter paper disks with broth culture and drying. Bacterial cultures can be stored in this mode for at least a year at 4 degrees C.
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