Biomedical subjects

Iwona Wilk

Publications and source records attributed to Iwona Wilk.

6 recordsLinked to original sources

PubMed2006

[The influence of AMPs from HaCaT keratinocytes on some bacteria strains derived from skin changes].

The aim of this study was to evaluate antimicrobial activity of protein extracts from HaCaT cell line against bacterial strains, isolated from clinical materials, obtained from patients with clinical symptoms of acne (Propionibacterium acnes) and gas gangrene (Clostridium perfringens and Sterptococcus pyogenes). Reference strain of Staphylococcus aureus ATCC 25923 also was used. Protein extracts from cultured HaCaT cells were obtained by 3-fold freezing/defreezing cells in dry ice following by centrifugation and incubated with appropriate bacterial suspension (0.5 McFarland scale) during 6 and 24 hours. We observed time-depending and strain-depending activity of HaCaT--protein extract. Interestingly, high activity was demonstrated against strains of S. pyogenes and C. perfringens. Because of increasing bacterial resistance to antibiotics further studies in the field of antimicrobial peptides are required.

Acne Vulgaris↗

PubMed2005

Inhibition of cell differentiation by quinolones in micromass cultures of rat embryonic limb bud and midbrain cells.

Micromass cultures (MMC) of rat embryonic limb bud (LB) and midbrain (CNS) cells were applied to compare the developmental toxicity of three quinolone antimicrobials: norfloxacin (Nor), enrofloxacin (Enr) and ciprofloxacin (Cip). Cultures were exposed for 5 days to seven concentrations of drugs. Cytotoxicity was assessed by quantifying neutral red uptake; differentiation-by quantifying alcian blue uptake (LB) or by image analysis of Gill's haematoxylin stained foci (CNS). Both, LB and CNS cultures showed dose-dependent reduction in total cell number and differentiation. To distinguish specific effect on differentiation, IC(50) for proliferation (P) and differentiation (D) were calculated and P/D ratios were compared. In LB cultures all three drugs were cytotoxic (P/D ratios were 1). In CNS cultures P/D ratios were 1 (up to 2.7 for Nor, up to 4.4 for Enr and up to 16 for Cip) what can suggest specific action on differentiation. Ciprofloxacin was the most toxic and CNS cells were more sensitive than LB. The ranges of IC(50)-D values (microg/ml) were as follows: Nor (79-14), Enr (127-179), Cip (91-101) in LB cultures; Nor (22-52), Enr (38-91), Cip (3-17) in CNS cultures. With one exception (Cip in CNS culture) all drugs were classified as weak embryotoxic.

Animals↗

PubMed2005

Co-occurrence of urogenital mycoplasmas and group B streptococci with chlamydial cervicitis.

The aim of our study was to evaluate whether in women with chlamydial cervicitis urogenital mycoplasmas and group B streptococci (GBS) colonization is found more often than among women with non-chlamydial cervicitis. This study included 351 (mean age 31.7 +/- 6.82) not pregnant, menstruating, sexually active women. We confirmed a high frequency (49.3%) of C. trachomatis infection among women with cervicitis. Cervical ectopia was confirmed in 26.5% of examined women, in half of them ectopia was associated with chlamydial infection. We did not notice differences in frequency of colonization by urogenital mycoplasmas and GBS among women with chlamydial and non-chlamydial cervicitis.

Adult↗

PubMed2004

[Nanobacteria--microbiological characteristics].

We have reviewed recent publications regarding the microbiological characteristic and pathogenicity of a novel infectious agent, the mineral-forming, sterile-filterable, slow-growing Gram-negative Nanobacteria, detected in bovine/human blood, kidney cyst fluid, urine and kidney stones. According to their 16S rDNA structure, nanobacteria belong to the alpha-2 Proteobacteria, subgroup, which includes the Brucella and Bartonella species. Their cell diameter is 0.2-0.5 microm (the smallest known cell-walled bacteria). Their most remarkable characteristic is the formation of carbonate apatite crystals of neutral pH and at physiologic phosphate and calcium concentrations. The extracellular mineralization forms a hard protective shelter for these hardy microorganisms, and enables them to survive conditions of physical stress that would be lethal to most other bacterial species. The Olavi Kajander group (Finland) suggests that the apatite produced by nanobacteria may play a key role in the formation of all kidney stones, by providing a central calcium phosphate deposit around which other crystalline components can collect. Nanobacteria seems to be a causative agent of diseases related to biomineralization processes.

Animals↗

PubMed2002

[Incidence of selected bacterial pathogens of the respiratory tract in patients with bronchial asthma].

Chronic bacterial infections intensify the reactivity of bronchi and aggravate the course and the control of asthma. They cause the disorders of both function and the structure of respiratory epithelium. Not only structural elements of bacteria but also their toxins intensify the release of mediators of the inflammatory reaction (leucotriens, histamine, IL1, IL4, IL6, IL8, TNF alpha). The aim of our research is to determine the prevalence of microorganisms, which can have an influence on the course of asthma. Moraxella catarrhalis has been the most frequent isolated pathogen (23.7%) in patients with bronchial asthma. We have received only individual isolations of Streptococcus pneumoniae and Haemophilus influenzae strains. Bacterial flora of the upper respiratory tract in patients with bronchial asthma has been more diverse in comparison with microflora of airways in healthy subjects. The significant percentage of Candida isolation in asthmatics (over 30% in bronchial tree secretion) poses the high risk of incidence of mycotic complications of inhaled steroids. In patients with asthma bronchial tree secretion is more valuable diagnostic material than pharyngeal swab.

Asthma↗

PubMed2002

[Effect of health care reform on the nurses' opinion employed in different health care institutions].

In the new system of health care nurses are not only doctors' partners carrying out doing their orders in the therapeutic process, but can also take up professional actions providing independently, totally and permanently, nursing care for the family and its individual members in their own environment, in the situation of health, illness, disability as well as home hospitalisation and terminal care. The study whose aim was to determine the professional position of nurses resulting from the change of work from time-based to contract task-based in connection with health care system reform was performed from September to November 2001 among nurses living in seven country communes of Kozienice county rendering different ranges of services in basic health care and among patients under nursing care communes under study. To solve research problems of the study the method of diagnostic survey was used with the technique of questionnaire. The study comprising 95 nurses and 112 patients has shown that introduction of contracting nursing services, connected with greater professional independence, possibility of organising own working time and increase of responsibility for performed tasks has increased the number and quality of offered services and also that contracting nursing services has contributed to an increase of professional prestige and nurses' satisfaction.

Adult↗