Biomedical subjects

J A Bourne

Publications and source records attributed to J A Bourne.

7 recordsLinked to original sources

PubMed2004

Single-unit responses to kinetic stimuli in New World monkey area V2: physiological characteristics of cue-invariant neurones.

In order to investigate the neural processes underlying figure-ground segregation on the basis of motion, we studied the responses of neurones in the second visual area (V2) of marmoset monkeys to stimuli that moved against dynamic textured backgrounds. The stimuli were either "solid" bars, which were uniformly darker or lighter than the background's average, or kinetic ("camouflaged") bars, formed by textural elements that matched the spatial and temporal modulation of the background. Camouflaged bars were rendered visible only by the coherent motion of their textural elements. Using solid bars, we subdivided the population of marmoset V2 neurones into motion-selective (uni- and bi-directional units, 73.3% of the sample) and weakly-biased (26.7%) subpopulations. The motion selective subpopulation was further subdivided into cue-invariant neurones (units which demonstrated a similar selectivity for the direction of motion of the solid and camouflaged bars) and non-cue-invariant neurones (units which showed selectivity to the direction of motion of solid bars, but had weak or pandirectional responses to camouflaged bars). Cells with cue-invariant responses to these stimuli were as common in V2 as in the primary visual area (V1; approximately 40% of the population). In V2, neurones with cue-invariant and non-cue-invariant motion selectivity formed distinct populations in terms of classical response properties: cue-invariant neurones were characterized by a sharp axis of motion selectivity and extensive length summation, while the majority of non-cue-invariant neurones had broader motion selectivity and were end-stopped. In the light of previous studies, these different constellations of classical response properties suggest a correlation with more traditionally recognized categories of V2 units and modular compartments. The responses of V2 cells to kinetic stimuli were slightly delayed relative to their responses to luminance-defined stimuli.

Action Potentials↗

PubMed2004

First- and second-order stimulus length selectivity in New World monkey striate cortex.

Motion is a powerful cue for figure-ground segregation, allowing the recognition of shapes even if the luminance and texture characteristics of the stimulus and background are matched. In order to investigate the neural processes underlying early stages of the cue-invariant processing of form, we compared the responses of neurons in the striate cortex (V1) of anaesthetized marmosets to two types of moving stimuli: bars defined by differences in luminance, and bars defined solely by the coherent motion of random patterns that matched the texture and temporal modulation of the background. A population of form-cue-invariant (FCI) neurons was identified, which demonstrated similar tuning to the length of contours defined by first- and second-order cues. FCI neurons were relatively common in the supragranular layers (where they corresponded to 28% of the recorded units), but were absent from layer 4. Most had complex receptive fields, which were significantly larger than those of other V1 neurons. The majority of FCI neurons demonstrated end-inhibition in response to long first- and second-order bars, and were strongly direction selective. Thus, even at the level of V1 there are cells whose variations in response level appear to be determined by the shape and motion of the entire second-order object, rather than by its parts (i.e. the individual textural components). These results are compatible with the existence of an output channel from V1 to the ventral stream of extrastriate areas, which already encodes the basic building blocks of the image in an invariant manner.

Action Potentials↗

PubMed2001

Changes in striatal electroencephalography and neurochemistry induced by kainic acid seizures are modified by dopamine receptor antagonists.

We investigated the involvement of striatal dopamine release in electrographic and motor seizure activity evoked by kainic acid in the guinea pig. The involvement of the dopamine receptor subtypes was studied by systemic administration of the dopamine D(1) receptor antagonist, R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzazepine hydrochloride (SCH 23390; 0.5 mg kg(-1)), or the dopamine D(2) antagonist, (5-aminosulphonyl)-N-[(1-ethyl-2-pyrrolidinyl)-methyl]-2-methoxybenzamide (sulpiride, 30 mg kg(-1)). Microdialysis and high performance liquid chromatography were used to monitor changes in extracellular levels of striatal dopamine and its metabolites, glutamate, aspartate and gamma-amino-butyric acid (GABA). These data were correlated with changes in the striatal and cortical electroencephalographs and clinical signs. We found that, although neither dopamine receptor antagonist inhibited behavioural seizure activity, blockade of the dopamine D(1)-like receptor with SCH 23390 significantly reduced both the 'power' of the electrical seizure activity and the associated change in extracellular striatal concentration of glutamate, whilst increasing the extracellular striatal concentration of GABA. In contrast, blockade of the dopamine D(2)-like receptor with sulpiride significantly increased the extracellular, striatal content of glutamate and the dopamine metabolites. These results confirm previous evidence in other models of chemically-evoked seizures that antagonism of the dopamine D(1) receptor tends to reduce motor and electrographic seizure activity as well as excitatory amino-acid transmitter activity, while antagonism of the dopamine D(2) receptor has relatively less apparent effect.

3,4-Dihydroxyphenylacetic Acid↗

PubMed2001

SCH 23390 affords protection against soman-evoked seizures in the freely moving guinea-pig: a concomitant neurochemical, electrophysiological and behavioural study.

We studied the role of striatal dopamine (DA) release in seizure activity evoked by the subcutaneous administration of the cholinesterase inhibitor pinacolyl methylphosphonofluoridate (soman), in the guinea-pig. The involvement of the dopamine receptor subtypes was studied by systemic administration of the D(1)-like receptor antagonist SCH 23390 (0.5 mg kg(-1)) or the D(2)-like receptor antagonist sulpiride (30 mg kg(-1)). Microdialysis and HPLC with electrochemical detection were used to monitor changes in extracellular levels of striatal DA and its metabolites, acetylcholine and choline. These data were correlated with changes in the striatal and cortical electroencephalogram and observation of predefined clinical signs. We found that the blockade of the D(1) receptor with SCH 23390 can inhibit seizure activity, while blockade of the D(2) receptor with sulpiride can augment the evoked seizure activity. These results clarify the involvement of the dopaminergic system in soman-evoked seizures.

Animals↗

PubMed2001

SCH 23390: the first selective dopamine D1-like receptor antagonist.

SCH 23390, the halobenzazepine (R)-(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5- tetrahydro-1H-3-benzazepine, is a highly potent and selective dopamine D1-like receptor antagonist with a K(i) of 0.2 and 0.3 nM for the D1 and D5 dopamine receptor subtypes, respectively. In vitro, it also binds with high affinity to the 5-HT2 and 5-HT1C serotonin receptor subtypes. However, the doses required to induce a similar response in vivo are greater than 10-fold higher than those required to induce a D1-mediated response. Previous in vivo pharmacological studies with SCH 23390 have shown it to abolish generalized seizures evoked by the chemoconvulsants: pilocarpine and soman. These studies provide evidence of the potential importance of D1-like dopaminergic receptor mechanisms in facilitating the initiation and spread of seizures. The inference from a majority of studies is that the activation of dopamine D1 receptors facilitates seizure activity, whereas activation of D2 receptors may inhibit the development of seizures. SCH 23390 has also been used in studies of other neurological disorders in which the dopamine system has been implicated, such as psychosis and Parkinson's disease. Apart from the study of neurological disorders, SCH 23390 has been extensively used as a tool in the topographical determination of brain D1 receptors in rodents, nonhuman primates, and humans. In summary, SCH 23390 has been a major tool in gaining a better understanding of the role of the dopamine system, more specifically the D1 receptor, in neurological function and dysfunction.

Animals↗

PubMed2000

Novel method of monitoring electroencephalography at the site of microdialysis during chemically evoked seizures in a freely moving animal.

This paper covers the design, development and operation of a novel piece of equipment, based around the CMA/12 guide probe (Carnegie Medicin, Sweden), which offers a low cost alternative for monitoring EEG at the site of microdialysis in a freely moving animal. This equipment is entirely based on commercially available parts, and thus can be easily replicated. Moreover, it is less intrusive than earlier models, offering advantages for experiments in which behavioural testing or chronic monitoring is required. We illustrate its use in a study of changes in electrical seizure activity, in both cortex and basal nuclei, evoked by the administration of the chemoconvulsant soman. The inference from the many experimental paradigms looking at the mechanisms of chemoconvulsants is that paroxysmal discharges are a better correlate of seizure activity than behavioural signs. The correlation of the EEG with extracellular neurotransmitter data, over a period of hours post-injection of chemoconvulsant, allows the determination of whether extracellular neurotransmitter changes are a cause or consequence of the evoked electrical activity.

Animals↗

PubMed1977

Experience and latency to achieve stereopsis: a replication.

This study replicated and extended the findings of MacCracken and Hayes (1976). 10 students were presented the same complex stereogram for 5 trials daily over 2 nonconsecutive days, and latencies to achieve depth perception were recorded. Latencies decreased across 5 trials in the first session but were somewhat longer at the beginning of the second session than at the end of the first session.

Adolescent↗