Functional reconstruction of the shoulder with a latissimus dorsi pedicle flap and a hemiarthroplasty: a case report.
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Adult↗
Biomedical subjects
J A Ferrier
Publications and source records attributed to J A Ferrier.
Acute flexor superficialis avulsion.
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Acute Disease↗
Novel method of detecting single base substitutions in RNA molecules by differential melting behavior in solution.
We have developed a quick and reliable way of detecting point mutations in RNA molecules. This method involves melting RNA-RNA heteroduplexes of varying lengths in a series of tubes containing a stepwise salt or formamide gradient, followed by polyacrylamide gel electrophoresis to distinguish between single- and double-stranded RNA molecules. The manipulations required are technically simple, and the method is sensitive enough to detect destabilization of the highest melting domain of a dsRNA duplex by a single base mismatch. When this method is used in parallel with denaturing gradient gel electrophoresis, which detects point mutations in low-melting domains of duplexes, it should now be possible to rapidly screen for mutations located throughout the length of any RNA molecule whose wild-type sequence is known.
Base Composition↗