PubMed Health⌕ Search

Biomedical subjects

J A Freeberg

Publications and source records attributed to J A Freeberg.

3 recordsLinked to original sources

Photosynthetic Rates of Sporophytes and Gametophytes of the Fern, Todea barbara.

The photosynthetic rates of intact sporophytes or gametophytes of the fern Todea barbara grown in sterile culture were measured using an infrared gas analyzer. Sporophytes consisted of single whole plants with roots and leaves grown in tubes of agar. Gametophytes were grown as several plants covering the surface of the agar. Sporophytes had photosynthetic rates at light saturation of 8.50 microliters CO(2) per hour per milligram dry weight and 1,300 microliters CO(2) per hour per milligram chlorophyll, whereas rates for gametophytes were lower, 2.36 microliters CO(2) per hour per milligram dry weight and 236 microliters CO(2) per hour per milligram chlorophyll.O(2) inhibited the photosynthetic rates of both plants to a degree typical of C-3 plants. The light-saturated photosynthetic rates of sporophytes increased 42% and gametophytes increased 27% when the O(2) concentration was changed from 21 to 1%.

Journal Article↗

Blue light-induced Absorbance Changes in Membrane Fractions from Corn and Neurospora.

Blue light-induced absorbance changes were measured from differentially centrifuged membrane fractions from dark-grown coleoptiles of Zea mays L., and mycelia from an albino mutant of Neurospora crassa. Actinic irradiation caused changes in absorbance consistent with a flavinmediated reduction of a b-type cytochrome. Both corn and Neurospora showed similar light-minus-dark difference spectra, dose response curves, and kinetics of dark recovery after irradiation. The photoreducible cytochrome system from Neurospora showed the same distribution as the activity of a sodium-stimulated adenosine triphosphatase, thought to be a plasma membrane marker, in differential centrifugation experiments. The fraction showing the absorbance change did not co-sediment with the mitochondria, nor with the endoplasmic reticulum. Comparison of absorption spectra of fully oxidized, partially reduced, and fully reduced preparations showed that approximately a 30% reduction of the cytochromes involved with the process was needed to obtain the light-induced absorbance changes.

Journal Article↗