Structure of chromosome fibers and chromosomes.
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Cell Nucleus↗
Biomedical subjects
J A Huberman
Publications and source records attributed to J A Huberman.
Stimulation of T4 bacteriophage DNA polymerase by the protein product of T4 gene 32.
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Centrifugation, Density Gradient↗
Electron microscopy of DNA polymerase bound to DNA.
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Coliphages↗
Enzymatic synthesis of deoxyribonucleic acid. XXXV. A 3'-hydroxylribonucleotide binding site of Escherichia coli deoxyribonucleic acid polymerase.
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Arabinose↗
Enzymatic synthesis of deoxyribonucleic acid. XXX. Binding of triphosphates to deoxyribonucleic acid polymerase.
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Binding Sites↗
On the mechanism of DNA replication in mammalian chromosomes.
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Animals↗
Visualization of replicating mammalian and T4 bacteriophage DNA.
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Animals↗
Autoradiography of chromosomal DNA fibers from Chinese hamster cells.
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Animals↗
Isolation of metaphase chromosomes from HeLa cells.
The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes contain about 2.0 mg of acid-soluble protein, 2.7 mg of acid-insoluble protein and 0.66 mg of RNA for each milligram of DNA. The RNA bound to the isolated chromosomes consists mainly of ribosomal RNA, but there is also a significant amount of 45S RNA.
Cell Division↗
DNA topoisomerases in eukaryotes.
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DNA Topoisomerases, Type I↗