Biomedical subjects
Publications and source records attributed to J A Pouezat.
A rare family case of a healthy newborn and his mother with multiple natal teeth and oligodontia described. The baby had 12 teeth at birth, while dental records of his mother showed that she had 16 teeth at birth. Possible etiologies and previous reports about natal teeth are discussed.
The use of calcium phosphate biomaterials as a bone substitute necessitates the use of normative biocompatibility and biodegradation techniques which must be fast, simple and reproducible. In the present study, we have developed an in vitro model to study and to compare different calcium phosphate ceramics. After activation with 1,25-dihydroxy-vitamin D3 and phorbol 12,13-dibutyrate, the monoblastic U937 cells became multinucleated, expressed tartrate-resistant acid phosphatase and several markers of monocyte/macrophage differentiation. Activated U937 cells did not express the vitronectin receptor (VNR) (as revealed using monoclonal antibodies 23C6 or 13C2) but around 25% of the cells were strongly reactive with 211D, a novel monoclonal antibody that recognizes an osteoclast-specific membrane antigenic determinant. These cells remain active/viable with hydroxyapatite (HA) or beta-tricalcium phosphate (beta-TCP) ceramics. In conclusion, activated U937 cells are good candidates to use in a normative in vitro method to evaluate new biomaterials.
The role played by phosphophoryn, one of the major noncollagenous proteins of dentin extracellular matrix, in the mineralization process has not been fully characterized. The purpose of our work was to produce monoclonal antibodies (MAbs) against dentin phosphophoryn and to test their reactivity with primary culture of odontoblasts. Dentin phosphophoryn (DPP) was extracted after the mechanical dissociation of teeth and dialyzed against guanidine and EDTA solutions followed by CaC1(2) precipitation. These extracts were characterized by SDS-PAGE and staining with Coomassie blue and Stains-All. After immunization of mice with these extracts, we produced MAb 7G4, which reacted with dentin phosphophoryn as revealed by Western blot. MAb 7G4 reactivity was tested against a primary culture of pig odontoblasts, revealing filaments specifically stained by the anti-DPP antibody. This antibody will be of great interest to study the mineralization process and dental pulp reaction after capping with various calcium phosphate materials.
The aim of the study described in this article is to propose a suitable index for epidemiological surveys. The authors define the characteristics of a good index to evaluate a presumed pathology, dental fluorosis, whoever the examiner and whatever the circumstances. The earliest and most commonly used index is Dean's because it permits data comparison among studies. But it has shortcomings due to the low accuracy of its definitions. The TSIF proposed by Horowitz et al. does not bring any noteworthy improvement to the Dean index. The new TF index devised by Thylstrup & Fejerskov is a logical improvement of the Dean index and can reliably be compared with Dean's. The sensitivity of this index come from its 9 stages reflecting the histopathology and fluoride content in the enamel. At the moment it is the most outstanding index for evaluating the severity of fluorosis.
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