Biomedical subjects

J A Thibodeau

Publications and source records attributed to J A Thibodeau.

4 recordsLinked to original sources

PubMed1989

Nurse practitioners: factors affecting role performance.

This descriptive, correlational investigation was designed to examine the attitudes/values held by nurse practitioners concerning their roles, self-assessment of their skills/knowledge, and orientation toward a nursing or medical model to guide practice. The results indicate that the nurse practitioners in this sample have a high degree of confidence in their skills and knowledge and have a fairly strong nursing orientation in their practice. The great deal of variability in responses to items concerned with audit, quality assurance and financial aspects of the role have important implications for nurse practitioner education and practice.

Attitude of Health Personnel↗

PubMed1988

Developing an original tool for research.

An instrument must be tested for validity and reliability before it can be used to measure the variables of interest specified in the research design. If the instrument is not valid and reliable, then the results of a study have limited credibility or usefulness in practice. The process is meticulous, but the results are gratifying when new data on nurse practitioners can be generated.

Attitude of Health Personnel↗

PubMedDate not supplied

Degradation of adsorbed fibrinogen by surface-generated plasmin.

An investigation of the physical status of fibrinogen that has been adsorbed to glass and then eluted has been conducted. Exposure of Kabi fibrinogen to glass was carried out using a glass bead column experiment. The fibrinogen was eluted sequentially, first by 1 M Tris and then by SDS. The initially eluted fibrinogen showed considerable degradation (SDS polyacrylamide gel electrophoresis) while the later fractions were less degraded. Fibrinogen purified by chromatography on either DEAE-cellulose or Sepharose-lysine to remove plasminogen was less degraded. When purified plasminogen was added to fibrinogen, degradation of column eluates was very extensive in all eluted fractions. These results are interpreted in terms of a surface-mediated activation of plasminogen to plasmin followed by fibrinogenolysis. Although such an effect remains to be demonstrated as a general property of surfaces, it is speculated that surfaces may vary in their activation of fibrinolysis as well as their activation of clotting, and that maximization of fibrinolysis is a worthwhile goal in the development of blood-compatible surfaces.

Adsorption↗

PubMedDate not supplied

Identification of proteins adsorbed from human plasma to glass bead columns: plasmin-induced degradation of adsorbed fibrinogen.

In a bead column experiment, attempts have been made to identify the proteins adsorbed from plasma onto a glass surface. Proteins adsorbed after a 3-h contact time were eluted sequentially by 1 M tris buffer and SDS. Polyacrylamide gel electrophoresis of eluted proteins showed a multiplicity of components, and not all of these could be identified. Positive identifications were made by immunodiffusion against specific antibodies, band positions on electrophoresis gels, and location of radioactivity in gels when specific radiolabeled proteins were added to plasma. Proteins found were albumin, IgG, fibrinogen, plasminogen, and fibrinogen degradation products (FDP). A major component with an apparent molecular weight of 25,000 remains unidentified. It is unrelated to albumin, IgG, fibrinogen, factor XII, or plasminogen. Adsorbed fibrinogen was less degraded when experiments were performed with plasmas deficient in either plasminogen or factor XII. It is therefore concluded that FDP are formed by activation of adsorbed plasminogen, as was found previously for purified fibrinogen containing a trace of plasminogen. At least part of this activation is potentiated by the contact activation phase of plasma coagulation, in particular activated factor XII.

Adsorption↗