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J Adhikari

Publications and source records attributed to J Adhikari.

6 recordsLinked to original sources
PubMed2006

NAD+ mediated differential thermotolerance between chloroplastic and cytosolic L-myo-inositol-1-phosphate synthase from Diplopterygium glaucum (Thunb.) Nakai.

Relative thermotolerance of the enzyme, L-myo-inositol-1-phosphate synthase (MIPS; EC: 5.5.1.4), from the chloroplastic and cytosolic sources of Diplopterygium glaucum was studied. The purification involved streptomycin sulphate precipitation, ammonium sulphate fractionation, ion-exchange chromatography, and molecular sieve chromatography. After the final chromatography, 16.62% of chloroplastic and 13.47% of cytosolic MIPS could be recovered. Between 15 degrees C and 55 degrees C, the two forms of MIPS exhibited differential thermal stability, which is related to the presence of the MIPS co-factor, NAD+. Added NAD+ increased the lower thermotolerance of the chloroplastic MIPS and the removal of 'built-in' NAD+ decreased the higher thermal stability of the cytosolic MIPS.

Chloroplasts↗
PubMed1989

Phosphoinositides from human foetal brain during development.

An attempt has been made in the present study to look for the activity of the enzymes which catalyse the breakdown of phosphorylated metabolites both in acidic and alkaline pH from human foetal brain, in different gestational ages during development in 18 foetal samples. It was revealed that phosphatases were distributed throughout the brain and were operative from the onset of development. There was a tendency of increment of specific activity of enzymes with respect to the advancement of gestational period. This observation should be accounted as a prerequisite criteria for understanding phosphate meatabolism in human foetal organs primarily associated with glycolytic and gluconeogenic pathways.

Brain↗
PubMed1987

Chloroplast as a Locale of L-myo-Inositol-1-Phosphate Synthase.

Chloroplasts from 5 to 7 day old Vigna radiata seedling, grown under alternate light/dark conditions or from green Euglena gracilis Z. cells have been found to harbor L-myo-inositol-1-phosphate synthase (EC 5.5.1.4) activity. In contrast, dark-grown V. radiata seedlings, or streptomycin-bleached Euglena cells exhibit either reduced or no enzyme activity. An apparent enhancement of the chloroplastic inositol synthase by growth in presence of light is observed.

Journal Article↗
PubMed1983

Differences in thermal stability of the fetal and adult brain myoinositol-1-phosphate synthase. Probable involvement of NAD.

L-myo-inositol-1-phosphate synthase (EC 5.5.1.4) from mammalian fetal and adult brain differ considerably with respect to their stability towards different temperatures between 25-65 degrees C. This property has been found to be associated with the presence of the synthase co-factor, NAD, bound to the enzyme protein. The lower thermal stability of the fetal enzyme increases in presence of added NAD (0.8 mM) whereas the higher thermal stability of the adult brain enzyme declines when NAD is specifically removed from the enzyme.

Animals↗