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J Albouy

Publications and source records attributed to J Albouy.

8 recordsLinked to original sources
PubMed1999

[Epidemiologic study of lead contamination of children of occupationally exposed parents].

BACKGROUND: As part of the screening of infantile saturnism in France, a cross sectional study has been conducted among 125 children with professionally lead exposed parents, working in two plants, both located at the edge of a rural village. The aim of this study was to seek a lead contamination of these children by their own parents, who may bring back home after work lead particles on their clothes, hair or skin. METHODS: These children were compared for blood lead levels by logistic regression to a reference group made of forty seven nursery school children, in these villages, without any lead exposed parents. Blood lead levels (< or > or = 70 micrograms/l) were performed for both children group and compared by logistic regression. Moreover parent's blood lead levels (< or = or > 400 micrograms/l) and lead air concentrations measured at their own working places (< or = or > 100 micrograms/m3, available from occupational health services) were also compared to their children blood lead levels. RESULTS: Blood lead levels in the "exposed group" were significantly higher than in the control group (OR = 9.9 [3.6-27.3]), all the more so as there were several exposed people in children home. In the exposed group, children blood lead levels were both correlated with parents blood lead levels (OR = 8.3 [2.9-24.0]) and lead air concentration measured at their working places (OR = 4.2 [1.6-10.9]). CONCLUSIONS: These results suggest a lead contamination of children by their exposed parents, which made it necessary to strengthen individual and collective lead prevention measures in both plants.

Adult↗
PubMed1998

Relationship of the pelargonium flower break carmovirus (PFBV) coat protein gene with that of other carmoviruses.

The 3'-terminal 1500 nucleotides of the genome of pelargonium flower break carmovirus (PFBV) were sequenced from RT-PCR amplification products. One large ORF was found, encoding a 345 amino acid protein of Mr 37 kDa, which corresponds to the coat protein, as confirmed by immunoprecipitation of products of in vitro transcription and translation. The sequence also included the putative promoter of the coat protein gene subgenomic RNA, as well as its 5' and 3' untranslated regions. The PFBV coat protein was more similar to that of saguaro cactus virus and carnation mottle virus than to that of other carmoviruses. Despite the lower level of similarity of CP gene sequences compared to the RNA dependent RNA polymerase (RdRp) gene sequences of small icosahedral viruses used in taxonomic studies, PFBV CP sequence comparisons and alignments confirmed that PFBV is related to carmoviruses, tombusviruses and a dianthovirus, as previously concluded from the analysis of a PFBV RdRp gene fragment.

Base Sequence↗
PubMed1992

Effects of antisense oligodeoxynucleotide hybridization on in vitro translation of potato virus Y RNA.

Potato virus Y (PVY), a potyvirus, has an RNA genome containing 9704 nucleotides of which 185 belong to the 5' nontranslated region (NTR). Contrary to most eukaryotic mRNAs that have a cap structure, the potyvirus RNA has a genome-linked protein (VPg). In order to understand the mechanisms of PVY RNA translation initiation, hybrid-arrest translation was used to localize sequences involved in binding of proteins and/or ribosomes. The 5' NTR was fused to the beta-glucuronidase (GUS) reporter gene. Six antisense oligodeoxynucleotides were used for hybridization, and the efficiency of the in vitro translation of the hybridized mRNA was modified to different levels depending upon the position of the oligodeoxynucleotide used. The highest inhibition was obtained with an oligodeoxynucleotide hybridized to the 5' end. In addition, translation of GUS mRNA containing the PVY 5' NTR was greatly enhanced when this mRNA was capped. These results differ from those obtained with the tobacco etch virus (TEV) and three picornaviruses, but are similar to those obtained with capped mRNA.

Animals↗
PubMed1991

Nucleotide sequence of the 3' terminal region of lettuce mosaic potyvirus RNA shows a Gln/Val dipeptide at the cleavage site between the polymerase and the coat protein.

DNA complementary to the 3' terminal 1651 nucleotides of the genome of the common strain of lettuce mosaic virus (LMV-O) has been cloned and sequenced. Microsequencing of the N-terminus enabled localization of the coat protein gene in this sequence. It showed also that the LMV coat protein coding region is at the 3' end of the genome, and that the coat protein is processed from a larger protein by cleavage at an unusual Q/V dipeptide between the polymerase and the coat protein. This is the first report of such a site for cleavage of a potyvirus polyprotein, where only Q/A, Q/S, and Q/G cleavage sites have been reported. The LMV coat protein gene encodes a 278 amino acid polypeptide with a calculated Mr of 31,171 and is flanked by a region which has a high degree of homology with the putative polymerase and a 3' untranslated region of 211 nucleotides in length. Percentage of homology with the coat protein of other potyviruses confirms that LMV is a distinct member of this group. Moreover, amino acid homologies noticed with the coat protein of potexvirus, bymovirus, and carlavirus elongated plant viruses suggest a functional significance for the conserved domains.

Amino Acid Sequence↗